open access
Antieosinophil action of IL-12 in human polyp culture
open access
Abstract
Methods: Nasal polyps were obtained during routine surgery and were cultured in fragments of approximately 30 mg for 2, 6, and 15 days in RPMI 1640 in the absence or presence of IL-12. Afterwards a dose-dependency was tested at day 2 of culture. Polyp tissue from cultures was than processed to slides, stained with Giemsa and cells were counted in light microscopy (400x).
Results: Eosinophils represented 62,8 ± 21,3% of residing cells in nasal polyps at the day 0. IL-12 (1 μg/ml) caused a significant time-dependent decrease in the percentage of Eos after 2 and 6 days. The effect of IL-12 at day 2 was concentration-dependent: control, 28,2 ± 2,9; at 10 ng/ml, 13,9 ± 6,4 (n = 4, p < 0,05); at 100 ng/ml, 11,6 ± 2,1 (p < 0.01); at 1 μg/ml, 7,5 ± 1,5 (p < 0,005).
Conclusion: IL-12 acts as potent topical antieosinophilic agent. Its action can be seen in a cultured polyp environment. It is visible already after two days and is concentration-dependent. Further study is needed to elucidate tissue mechanisms of this action.
Abstract
Methods: Nasal polyps were obtained during routine surgery and were cultured in fragments of approximately 30 mg for 2, 6, and 15 days in RPMI 1640 in the absence or presence of IL-12. Afterwards a dose-dependency was tested at day 2 of culture. Polyp tissue from cultures was than processed to slides, stained with Giemsa and cells were counted in light microscopy (400x).
Results: Eosinophils represented 62,8 ± 21,3% of residing cells in nasal polyps at the day 0. IL-12 (1 μg/ml) caused a significant time-dependent decrease in the percentage of Eos after 2 and 6 days. The effect of IL-12 at day 2 was concentration-dependent: control, 28,2 ± 2,9; at 10 ng/ml, 13,9 ± 6,4 (n = 4, p < 0,05); at 100 ng/ml, 11,6 ± 2,1 (p < 0.01); at 1 μg/ml, 7,5 ± 1,5 (p < 0,005).
Conclusion: IL-12 acts as potent topical antieosinophilic agent. Its action can be seen in a cultured polyp environment. It is visible already after two days and is concentration-dependent. Further study is needed to elucidate tissue mechanisms of this action.
Keywords
eosinophil; interleukin 12; asthma; nasal polyps


Title
Antieosinophil action of IL-12 in human polyp culture
Journal
Advances in Respiratory Medicine
Issue
Pages
14-18
Published online
2008-02-18
Bibliographic record
Pneumonol Alergol Pol 2004;72(1-2):14-18.
Keywords
eosinophil
interleukin 12
asthma
nasal polyps
Authors
Andrzej M. Fal
Jerzy Rabczyński
Agnieszka Gerber
Wojciech Mikulewicz
Wojciech Barg
Andrzej Obojski