open access

Vol 74, No 1 (2006)
ORIGINAL PAPERS
Published online: 2008-02-18
Submitted: 2013-02-22
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Identificatiomn of Mycobacteriaceae species based on the hsp-65 gene polymorphism analysis by PCR-RFLP

Adam Frangrat, Renata Walkiewicz, Aleksandra Safianowska, Hanna Grubek-Jaworska, Ryszarda Chazan
Pneumonol Alergol Pol 2006;74(1):95-100.

open access

Vol 74, No 1 (2006)
ORIGINAL PAPERS
Published online: 2008-02-18
Submitted: 2013-02-22

Abstract

The polymorphism of the short fragment of the heat shock protein 65 encoding gene was evaluated by the PCR - RFLP technique described by Telenti and further developed by Devallois for identification of mycobacterial species in routine laboratory work. We analysed 58 strains representing 25 different mycobacterial species (24 reference strains and 34 clinical isolates). The results obtained by PCR-RFLP and HPLC identification techniques were highly concordant The results were compatible for 87,5% (21 / 24) reference strains and for 97,1% (33/34) clinical isolates. The PCR - RFLP method allowed for accurate identification mycobacterials pecies, especially pathogenic strains. Restriction patterns obtained for 25 species of Mycobacteriaceae genus could help in constructing the data base and algorithms used in routine laboratory practice.

Abstract

The polymorphism of the short fragment of the heat shock protein 65 encoding gene was evaluated by the PCR - RFLP technique described by Telenti and further developed by Devallois for identification of mycobacterial species in routine laboratory work. We analysed 58 strains representing 25 different mycobacterial species (24 reference strains and 34 clinical isolates). The results obtained by PCR-RFLP and HPLC identification techniques were highly concordant The results were compatible for 87,5% (21 / 24) reference strains and for 97,1% (33/34) clinical isolates. The PCR - RFLP method allowed for accurate identification mycobacterials pecies, especially pathogenic strains. Restriction patterns obtained for 25 species of Mycobacteriaceae genus could help in constructing the data base and algorithms used in routine laboratory practice.
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Keywords

tuberculous mycobacteria; atypical mycobacteria; PCR - RFLP; HPLC

About this article
Title

Identificatiomn of Mycobacteriaceae species based on the hsp-65 gene polymorphism analysis by PCR-RFLP

Journal

Advances in Respiratory Medicine

Issue

Vol 74, No 1 (2006)

Pages

95-100

Published online

2008-02-18

Bibliographic record

Pneumonol Alergol Pol 2006;74(1):95-100.

Keywords

tuberculous mycobacteria
atypical mycobacteria
PCR - RFLP
HPLC

Authors

Adam Frangrat
Renata Walkiewicz
Aleksandra Safianowska
Hanna Grubek-Jaworska
Ryszarda Chazan

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