open access

Vol 75, No 3 (2007)
ORIGINAL PAPERS
Published online: 2007-07-30
Submitted: 2013-02-22
Get Citation

Posttranslational p53 phosphorylation in non small cell lung cancer cells after radio/chemotherapy

Adam Hołownia, Robert Marek Mróz, Mirosław Kozłowski, Elżbieta Chyczewska, Jerzy Laudański, Lech Chyczewski, Jan Józef Braszko
Pneumonol Alergol Pol 2007;75(3):241-250.

open access

Vol 75, No 3 (2007)
ORIGINAL PAPERS
Published online: 2007-07-30
Submitted: 2013-02-22

Abstract

Introduction: p53 protein is a critical regulator of cell cycle and apoptosis. Many stimuli change its expression and modify its functions. The aim of our work was to determine stability and chosen posttranslational modification of p53 protein during the treatment of lung cancer.
Material and methods: We investigated levels of poly-ADP-ribose- a marker of cellular DNA damage, DNA ploidy, Ki-67 expression, wild type and mutated p53 protein expression and intensity of phosphorylation of chosen p53 serine sites: C-terminal Ser392, and N-terminal Ser15, and Ser20 in fiberoptic bronchoscopy biopsy samples taken from patients suffering from recurrent squamous cell lung cancer before and after radio/chemotherapy. Analysis was based on results obtained from 23 patients after surgery in I-IIIA clinical stage of the disease.
Results: Therapy lowered the number of G2/M cells, but increased S fraction cell population in about 50%. Therapy increased p53 expression and p53 phosphorylation at Ser392 and Ser20, and these changes correlated with poly-ADP-ribose levels and Ki-67 expression.
Conclusions: Our results indicate that apart from changes in p53 quantity, p53 posttranslational phosphorylation play a role in regulation of neoplastic cells proliferation in response to drugs.

Abstract

Introduction: p53 protein is a critical regulator of cell cycle and apoptosis. Many stimuli change its expression and modify its functions. The aim of our work was to determine stability and chosen posttranslational modification of p53 protein during the treatment of lung cancer.
Material and methods: We investigated levels of poly-ADP-ribose- a marker of cellular DNA damage, DNA ploidy, Ki-67 expression, wild type and mutated p53 protein expression and intensity of phosphorylation of chosen p53 serine sites: C-terminal Ser392, and N-terminal Ser15, and Ser20 in fiberoptic bronchoscopy biopsy samples taken from patients suffering from recurrent squamous cell lung cancer before and after radio/chemotherapy. Analysis was based on results obtained from 23 patients after surgery in I-IIIA clinical stage of the disease.
Results: Therapy lowered the number of G2/M cells, but increased S fraction cell population in about 50%. Therapy increased p53 expression and p53 phosphorylation at Ser392 and Ser20, and these changes correlated with poly-ADP-ribose levels and Ki-67 expression.
Conclusions: Our results indicate that apart from changes in p53 quantity, p53 posttranslational phosphorylation play a role in regulation of neoplastic cells proliferation in response to drugs.
Get Citation

Keywords

p53; DNA ploidy; non-small cell lung cancer; cisplatin; vinorelbine

About this article
Title

Posttranslational p53 phosphorylation in non small cell lung cancer cells after radio/chemotherapy

Journal

Advances in Respiratory Medicine

Issue

Vol 75, No 3 (2007)

Pages

241-250

Published online

2007-07-30

Bibliographic record

Pneumonol Alergol Pol 2007;75(3):241-250.

Keywords

p53
DNA ploidy
non-small cell lung cancer
cisplatin
vinorelbine

Authors

Adam Hołownia
Robert Marek Mróz
Mirosław Kozłowski
Elżbieta Chyczewska
Jerzy Laudański
Lech Chyczewski
Jan Józef Braszko

Important: This website uses cookies. More >>

The cookies allow us to identify your computer and find out details about your last visit. They remembering whether you've visited the site before, so that you remain logged in - or to help us work out how many new website visitors we get each month. Most internet browsers accept cookies automatically, but you can change the settings of your browser to erase cookies or prevent automatic acceptance if you prefer.

Czasopismo Pneumonologia i Alergologia Polska dostęne jest również w Ikamed - księgarnia medyczna

Wydawcą serwisu jest "Via Medica sp. z o.o." sp.k., ul. Świętokrzyska 73, 80–180 Gdańsk

tel.:+48 58 320 94 94, faks:+48 58 320 94 60, e-mail: viamedica@viamedica.pl