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Vol 77, No 1 (2009)
ORIGINAL PAPERS
Published online: 2008-12-19
Submitted: 2013-02-22
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Association of A/T polymorphism of the CHRM2 gene with bronchodilator response to ipratropium bromide in asthmatic children

Aleksandra Szczepankiewicz, Anna Bręborowicz, Paulina Sobkowiak, Lucyna Kramer, Anna Popiel
Pneumonol Alergol Pol 2009;77(1):5-10.

open access

Vol 77, No 1 (2009)
ORIGINAL PAPERS
Published online: 2008-12-19
Submitted: 2013-02-22

Abstract


Introduction: The aim of this study was to analyze the possible association of A/T polymorphism of the CHRM2 gene with asthma, and pharmacogenetic analysis of the polymorphism with bronchodilator response to ipratropium bromide, an anticholinergic drug used in asthma.
Material and methods: Analysis was performed in a group of 113 children diagnosed with bronchial asthma, and in a group of 123 healthy children from a control group. Moreover, in the group of 32 asthmatic children without concurrent treatment with long-acting β2-agonists, bronchodilator response to ipratropium bromide was evaluated by the spirometric lung function test. Genetic analysis was performed for A/T polymorphism (rs6962027) of the CHRM2 gene. Genotyping was done with the PCR-RFLP method. Statistical analysis was performed using Statistica v.7.1 software.
Results: No association of A/T polymorphism was found with asthma (p = 0.865 for genotypes and p = 0.782 for alleles). In the pharmacogenetic analysis, it was observed that patients carrying TT genotype of CHRM2 gene polymorphism demonstrated significantly poorer response to anticholinergic drug as compared to the patients with other genotypes for this polymorphism (p = 0.035).
Conclusions: We found that TT genotype in the CHRM2 gene was associated with poor bronchodilator response in asthmatic patients. The results should be analyzed carefully considering the small sample size and should be confirmed by other research groups.

Abstract


Introduction: The aim of this study was to analyze the possible association of A/T polymorphism of the CHRM2 gene with asthma, and pharmacogenetic analysis of the polymorphism with bronchodilator response to ipratropium bromide, an anticholinergic drug used in asthma.
Material and methods: Analysis was performed in a group of 113 children diagnosed with bronchial asthma, and in a group of 123 healthy children from a control group. Moreover, in the group of 32 asthmatic children without concurrent treatment with long-acting β2-agonists, bronchodilator response to ipratropium bromide was evaluated by the spirometric lung function test. Genetic analysis was performed for A/T polymorphism (rs6962027) of the CHRM2 gene. Genotyping was done with the PCR-RFLP method. Statistical analysis was performed using Statistica v.7.1 software.
Results: No association of A/T polymorphism was found with asthma (p = 0.865 for genotypes and p = 0.782 for alleles). In the pharmacogenetic analysis, it was observed that patients carrying TT genotype of CHRM2 gene polymorphism demonstrated significantly poorer response to anticholinergic drug as compared to the patients with other genotypes for this polymorphism (p = 0.035).
Conclusions: We found that TT genotype in the CHRM2 gene was associated with poor bronchodilator response in asthmatic patients. The results should be analyzed carefully considering the small sample size and should be confirmed by other research groups.
Citation Formats

Keywords

ipratropium bromide; asthma; muscarinic type 2 receptor gene (CHRM2); polymorphism; bronchodilator response

About this article
Title

Association of A/T polymorphism of the CHRM2 gene with bronchodilator response to ipratropium bromide in asthmatic children

Journal

Advances in Respiratory Medicine

Issue

Vol 77, No 1 (2009)

Pages

5-10

Published online

2008-12-19

Bibliographic record

Pneumonol Alergol Pol 2009;77(1):5-10.

Keywords

ipratropium bromide
asthma
muscarinic type 2 receptor gene (CHRM2)
polymorphism
bronchodilator response

Authors

Aleksandra Szczepankiewicz
Anna Bręborowicz
Paulina Sobkowiak
Lucyna Kramer
Anna Popiel

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