open access

Vol 78, No 6 (2010)
ORIGINAL PAPERS
Published online: 2010-10-29
Submitted: 2013-02-22
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The results of molecular epidemiological investigations in patients infected with strains of the genus Acinetobacter

Joanna Nowak, Agnieszka Pacholczyk, Violetta Petroniec, Beata Dziedzicka, Zofia Zwolska
Pneumonol Alergol Pol 2010;78(6):386-391.

open access

Vol 78, No 6 (2010)
ORIGINAL PAPERS
Published online: 2010-10-29
Submitted: 2013-02-22

Abstract


Introduction: Acinetobacter spp. is an important opportunistic pathogen responsible for increasing number of nosocomial infections. The majority of infection are of epidemic origin, and treatment has become difficult because many strains are resistant to a wide range of antibiotics. The aim of this study was to investigate the local infections caused by various species of the genus Acinetobacter, occurring in the hospital wards IGiChP in periods of increased prevalence: August 2007 and February and March 2008.
Material and methods: Twenty three strains of Acinetobacter spp. were isolated from 19 patients residing in the same period and the same hospital ward (2007 - 13 strains from 11 patients, 2008 - 10 strains from 8 patients). Acinetobacter isolates obtained from these patients were characterized by phenotypically methods and genotypically by arbitrarily primed PCR (AP-PCR).
Results: All strains of Acinetobacter (n = 23) were multi-drug resistant. Used AP-PCR method showed 10 genotypes among the all strains. Acinetobacter spp. strains cultivated in 2007 and 2008 belonged to one genotype, came from patients hospitalized on the same wards, which confirms the transmission of infection in the patient’s residence.
Conclusions: The same genotype Acinetobacter baumannii strains isolated from two patients in 2007, and two patients in 2008, showed the presence of bacteria in the hospital environment. In the present study, we also established the usefulness of AP-PCR in molecular epidemiological investigations.
Pneumonol. Alergol. Pol. 2010; 78, 6: 386-391

Abstract


Introduction: Acinetobacter spp. is an important opportunistic pathogen responsible for increasing number of nosocomial infections. The majority of infection are of epidemic origin, and treatment has become difficult because many strains are resistant to a wide range of antibiotics. The aim of this study was to investigate the local infections caused by various species of the genus Acinetobacter, occurring in the hospital wards IGiChP in periods of increased prevalence: August 2007 and February and March 2008.
Material and methods: Twenty three strains of Acinetobacter spp. were isolated from 19 patients residing in the same period and the same hospital ward (2007 - 13 strains from 11 patients, 2008 - 10 strains from 8 patients). Acinetobacter isolates obtained from these patients were characterized by phenotypically methods and genotypically by arbitrarily primed PCR (AP-PCR).
Results: All strains of Acinetobacter (n = 23) were multi-drug resistant. Used AP-PCR method showed 10 genotypes among the all strains. Acinetobacter spp. strains cultivated in 2007 and 2008 belonged to one genotype, came from patients hospitalized on the same wards, which confirms the transmission of infection in the patient’s residence.
Conclusions: The same genotype Acinetobacter baumannii strains isolated from two patients in 2007, and two patients in 2008, showed the presence of bacteria in the hospital environment. In the present study, we also established the usefulness of AP-PCR in molecular epidemiological investigations.
Pneumonol. Alergol. Pol. 2010; 78, 6: 386-391
Get Citation

Keywords

Acinetobacter spp.; nosocomial infections; epidemiological investigations; AP-PCR

About this article
Title

The results of molecular epidemiological investigations in patients infected with strains of the genus Acinetobacter

Journal

Advances in Respiratory Medicine

Issue

Vol 78, No 6 (2010)

Pages

386-391

Published online

2010-10-29

Bibliographic record

Pneumonol Alergol Pol 2010;78(6):386-391.

Keywords

Acinetobacter spp.
nosocomial infections
epidemiological investigations
AP-PCR

Authors

Joanna Nowak
Agnieszka Pacholczyk
Violetta Petroniec
Beata Dziedzicka
Zofia Zwolska

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