open access

Vol 81, No 2 (2013)
ORIGINAL PAPERS
Published online: 2013-02-18
Submitted: 2013-02-22
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Analysis of the frequency of isolation and drug resistance of microorganisms isolated from the airways of adult CF patients treated in the Institute of Tuberculosis and Lung Disease during 2008–2011

Agnieszka Iwańska, Joanna Nowak, Wojciech Skorupa, Ewa Augustynowicz-Kopeć
Pneumonol Alergol Pol 2013;81(2):105-113.

open access

Vol 81, No 2 (2013)
ORIGINAL PAPERS
Published online: 2013-02-18
Submitted: 2013-02-22

Abstract

INTRODUCTION: Cystic fibrosis (CF) is the most common genetic autosomal recessive genetic disease. The most serious symptoms are observed in the lungs. Recurrent respiratory infections are the main causes of the hospitalizations and deaths of cystic fibrosis patients. Pathogens that commonly infect the airways of adult CF patients include Staphylococcus aureus and Pseudomonas aeruginosa. The aim of this study was to analyse the microorganisms cultured from the airways of adult CF patients and to test the antimicrobial resistance of the most frequently isolated bacteria.

MATERIAL AND METHODS: In this study, 1422 isolates of 89 CF patients were collected during a 4-year period. The microorganisms were cultured and identified according to standard microbiological procedures. Identification and drug susceptibility were performed in an automatic system Phoenix (BD), Vitek2Compact (bioMérieux), and disk-diffusion method by Kirby-Bauer.

RESULTS: Among the 1422 strains the most frequent pathogens were Pseudomonas aeruginosa (55.6%) and Staphylococcus aureus (37.8%). A total of 482 (61.0%) strains of 790 isolates of P. aeruginosa, were identified as P. aeruginosa mucoid phenotype. The isolates with mucoid phenotypes were more susceptible to antibiotics than non-mucoid. Eighty-six strains of S. aureus showed resistance to methicillin (MRSA), which accounted for 16.0% of all strains of S. aureus.

CONCLUSIONS: The analysis of microbiological materials from adult CF patients treated in IGiChP allowed the determination of the prevalence of potentially pathogenic microorganisms. The data obtained are consistent with the literature.

Abstract

INTRODUCTION: Cystic fibrosis (CF) is the most common genetic autosomal recessive genetic disease. The most serious symptoms are observed in the lungs. Recurrent respiratory infections are the main causes of the hospitalizations and deaths of cystic fibrosis patients. Pathogens that commonly infect the airways of adult CF patients include Staphylococcus aureus and Pseudomonas aeruginosa. The aim of this study was to analyse the microorganisms cultured from the airways of adult CF patients and to test the antimicrobial resistance of the most frequently isolated bacteria.

MATERIAL AND METHODS: In this study, 1422 isolates of 89 CF patients were collected during a 4-year period. The microorganisms were cultured and identified according to standard microbiological procedures. Identification and drug susceptibility were performed in an automatic system Phoenix (BD), Vitek2Compact (bioMérieux), and disk-diffusion method by Kirby-Bauer.

RESULTS: Among the 1422 strains the most frequent pathogens were Pseudomonas aeruginosa (55.6%) and Staphylococcus aureus (37.8%). A total of 482 (61.0%) strains of 790 isolates of P. aeruginosa, were identified as P. aeruginosa mucoid phenotype. The isolates with mucoid phenotypes were more susceptible to antibiotics than non-mucoid. Eighty-six strains of S. aureus showed resistance to methicillin (MRSA), which accounted for 16.0% of all strains of S. aureus.

CONCLUSIONS: The analysis of microbiological materials from adult CF patients treated in IGiChP allowed the determination of the prevalence of potentially pathogenic microorganisms. The data obtained are consistent with the literature.

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Keywords

antimicrobial resistance; cystic fibrosis; Pseudomonas aeruginosa; Staphylococcus aureus

About this article
Title

Analysis of the frequency of isolation and drug resistance of microorganisms isolated from the airways of adult CF patients treated in the Institute of Tuberculosis and Lung Disease during 2008–2011

Journal

Advances in Respiratory Medicine

Issue

Vol 81, No 2 (2013)

Pages

105-113

Published online

2013-02-18

Bibliographic record

Pneumonol Alergol Pol 2013;81(2):105-113.

Keywords

antimicrobial resistance
cystic fibrosis
Pseudomonas aeruginosa
Staphylococcus aureus

Authors

Agnieszka Iwańska
Joanna Nowak
Wojciech Skorupa
Ewa Augustynowicz-Kopeć

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