open access
Role of plasma 8-OXO-2’-deoxyguanosine in target organ damage in patients with hypertension and type 2 diabetes
Affiliations- Educational and Scientific Medical Institute of National Technical University “Kharkiv Polytechnic Institute”, Kharkiv, Ukraine
- Kharkiv National Medical University, Kharkiv, Ukraine
- Government Institution ‘L.T. Malaya Therapy National Institute of the National Academy of Medical Sciences of Ukraine’, Kharkiv, Ukraine
- National Aerospace University H.E. Zhukovsky “Kharkiv Aviation Institute”, Kharkiv, Ukraine
open access
Abstract
Background: The aim of the study was to determine the prognostic value of 8-oxo-2’-deoxyguanosine (8-OHdG) plasma levels for cardiovascular complications (CVC) development in hypertension (HTN) and type 2 diabetes mellitus (T2DM) comorbidity.
Material and methods: One hundred fifty-six patients (mean age 61.71 ± 0.87 years) with the combined course of HTN stage II and T2DM (group 1) and 100 non-diabetic patients with HTN stage II (mean age 60.59 ± 0.87 years, group 2) were examined. Lipid, carbohydrate metabolism, plasma insulin, plasma 8-OHdG (by ELISA), blood pressure levels were measured. Observation period was 12 months.
Results: Plasma levels of 8-OHdG in the patients’ groups were significantly higher than in the controls (p < 0.001), in the group 1 higher than in group 2 (15.37 ± 0.27 ng/L vs. 14.00 ± 0.29 ng/L, respectively, p = 0.002). Plasma levels of 8-OHdG in group-2 patients who developed cardiovascular complications during observation period were significantly higher than in those without cardiovascular complications (16.47 ± 0.62 ng/L vs. 15.11 ± 0.29 ng/L, respectively, p = 0.046). The ROC analysis made it possible to propose the 8-OHdG plasma level ≥ 15.68 ng/L as an identifier for the cardiovascular complications in patients with studied comorbidity. In group 1, in patients with 8-OHdG plasma level ≥ 15.68 ng/L such cardiovascular risk factors as total cholesterol (TC) (p = 0.034), low density lipoprotein cholesterol (LDL-C) (p = 0.035), systolic blood pressure (p = 0.022), HOMA-IR (p = 0.046) were significantly higher compared with those whose level of this indicator was < 15.68 ng/L.
Conclusion: Determination of the plasma 8-OHdG allows not only for assessment of the severity of oxidative stress, but also for determination of the course and prognosis in comorbidity of HTN and T2DM.
Abstract
Background: The aim of the study was to determine the prognostic value of 8-oxo-2’-deoxyguanosine (8-OHdG) plasma levels for cardiovascular complications (CVC) development in hypertension (HTN) and type 2 diabetes mellitus (T2DM) comorbidity.
Material and methods: One hundred fifty-six patients (mean age 61.71 ± 0.87 years) with the combined course of HTN stage II and T2DM (group 1) and 100 non-diabetic patients with HTN stage II (mean age 60.59 ± 0.87 years, group 2) were examined. Lipid, carbohydrate metabolism, plasma insulin, plasma 8-OHdG (by ELISA), blood pressure levels were measured. Observation period was 12 months.
Results: Plasma levels of 8-OHdG in the patients’ groups were significantly higher than in the controls (p < 0.001), in the group 1 higher than in group 2 (15.37 ± 0.27 ng/L vs. 14.00 ± 0.29 ng/L, respectively, p = 0.002). Plasma levels of 8-OHdG in group-2 patients who developed cardiovascular complications during observation period were significantly higher than in those without cardiovascular complications (16.47 ± 0.62 ng/L vs. 15.11 ± 0.29 ng/L, respectively, p = 0.046). The ROC analysis made it possible to propose the 8-OHdG plasma level ≥ 15.68 ng/L as an identifier for the cardiovascular complications in patients with studied comorbidity. In group 1, in patients with 8-OHdG plasma level ≥ 15.68 ng/L such cardiovascular risk factors as total cholesterol (TC) (p = 0.034), low density lipoprotein cholesterol (LDL-C) (p = 0.035), systolic blood pressure (p = 0.022), HOMA-IR (p = 0.046) were significantly higher compared with those whose level of this indicator was < 15.68 ng/L.
Conclusion: Determination of the plasma 8-OHdG allows not only for assessment of the severity of oxidative stress, but also for determination of the course and prognosis in comorbidity of HTN and T2DM.
Keywords
arterial hypertension; type 2 diabetes mellitus; plasma 8-oxo-2’-deoxyguanosine; cardiovascular complications
About this articleTitle
Role of plasma 8-OXO-2’-deoxyguanosine in target organ damage in patients with hypertension and type 2 diabetes
Journal
Issue
Article type
Original paper
Pages
78-83
Published online
2022-04-06
Page views
2116
Article views/downloads
244
DOI
10.5603/AH.a2022.0006
Bibliographic record
Arterial Hypertension 2022;26(2):78-83.
Keywords
arterial hypertension
type 2 diabetes mellitus
plasma 8-oxo-2’-deoxyguanosine
cardiovascular complications
Authors
Valeriya Nemtsova
Anna Shalimova
Olena Kolesnikova
Olena Vysotska
Vira Zlatkina
Natalia Zhelezniakova
References (25)- Forouzanfar MH, Alexander L, Anderson HR, et al. GBD 2013 Risk Factors Collaborators. Global, regional, and national comparative risk assessment of 79 behavioural, environmental and occupational, and metabolic risks or clusters of risks in 188 countries, 1990-2013: a systematic analysis for the Global Burden of Disease Study 2013. Lancet. 2015; 386(10010): 2287–2323.
- Illiash MH, Bazyka OIe, Dovhanych NV, et al. [Hypertension and diabetes mellitus: modern aspects of treatment]. Practitioner. 2016; 5(2): 5–9.
- Münzel T, Camici GG, Maack C, et al. Impact of Oxidative Stress on the Heart and Vasculature: Part 2 of a 3-Part Series. J Am Coll Cardiol. 2017; 70(2): 212–229.
- Kalinina EV, Chernov NN, Novichkova MD. Role of glutathione, glutathione transferase, and glutaredoxin in regulation of redox-dependent processes. Biochemistry (Mosc). 2014; 79(13): 1562–1583.
- Urbaniak SK, Boguszewska K, Szewczuk M, et al. 8-Oxo-7,8-Dihydro-2'-Deoxyguanosine (8-oxodG) and 8-Hydroxy-2'-Deoxyguanosine (8-OHdG) as a Potential Biomarker for Gestational Diabetes Mellitus (GDM) Development. Molecules. 2020; 25(1).
- Marmiy NV, Esipov DS. The biological role of 8-oxo-2'-deoxyguanosine. Moscow University Bulletin Series 16 Biology. 2015; 4: 19–23.
- Al-Aubaidy HA, Jelinek HF. Oxidative DNA damage and obesity in type 2 diabetes mellitus. Eur J Endocrinol. 2011; 164(6): 899–904.
- Di Minno A, Turnu L, Porro B, et al. 8-Hydroxy-2-Deoxyguanosine Levels and Cardiovascular Disease: A Systematic Review and Meta-Analysis of the Literature. Antioxid Redox Signal. 2016; 24(10): 548–555.
- Kant M, Akış M, Çalan M, et al. Elevated urinary levels of 8-oxo-2'-deoxyguanosine, (5'R)- and (5'S)-8,5'-cyclo-2'-deoxyadenosines, and 8-iso-prostaglandin F as potential biomarkers of oxidative stress in patients with prediabetes. DNA Repair (Amst). 2016; 48: 1–7.
- Williams B, Mancia G, Spiering W, et al. ESC Scientific Document Group . 2018 ESC/ESH Guidelines for the management of arterial hypertension. Eur Heart J. 2018; 39(33): 3021–3104.
- Buse JB, Wexler DJ, Tsapas A, et al. 2019 update to: Management of hyperglycaemia in type 2 diabetes, 2018. A consensus report by the American Diabetes Association (ADA) and the European Association for the Study of Diabetes (EASD). Diabetologia. 2020; 63(2): 221–228.
- Unifikovanyi klinichnyi protokol pervynnoi ta vtorynnoi (spetsializovanoi) medychnoi dopomohy: tsukrovyi diabet 2 typu (nakaz MOZ 1118 vid 21.12.2012 r. [In Ukrainian].
- Arutyunov A, Dubinina EE, Zyibina NN. [Methods for evaluation of free radical oxidation and antioxidant system of the body: methodical recommendations] . Foliant. 2004: 104c.
- Liu A, Wu Q, Guo J, et al. Statins: Adverse reactions, oxidative stress and metabolic interactions. Pharmacol Ther. 2019; 195: 54–84.
- Zeng H, Liu Z. Atorvastatin Induces Hepatotoxicity in Diabetic Rats via Oxidative Stress, Inflammation, and Anti-Apoptotic Pathway. Med Sci Monit. 2019; 25: 6165–6173.
- Nemtsova VD. The effect of statin therapy on oxidative stress indices in patients with arterial hypertension and type 2 diabetes mellitus. Zaporozhye Med J. 2018; 0(1).
- Lorza-Gil E, de Souza JC, García-Arévalo M, et al. Coenzyme Q protects against β-cell toxicity induced by pravastatin treatment of hypercholesterolemia. J Cell Physiol. 2019; 234(7): 11047–11059.
- Thomas MC, Woodward M, Li Q, et al. ADVANCE Collaborative Group. Relationship Between Plasma 8-OH-Deoxyguanosine and Cardiovascular Disease and Survival in Type 2 Diabetes Mellitus: Results From the ADVANCE Trial. J Am Heart Assoc. 2018; 7(13).
- Yıldırım E, İpek E, Bavunoğlu I, et al. The impact of protein oxidation on sustained and white coat hypertension. Anatol J Cardiol. 2017; 17(3): 210–216.
- Di Minno A, Turnu L, Porro B, et al. 8-Hydroxy-2-Deoxyguanosine Levels and Cardiovascular Disease: A Systematic Review and Meta-Analysis of the Literature. Antioxid Redox Signal. 2016; 24(10): 548–555.
- Al-Aubaidy HA, Jelinek HF. Oxidative DNA damage and obesity in type 2 diabetes mellitus. Eur J Endocrinol. 2011; 164(6): 899–904.
- Urbaniak SK, Boguszewska K, Szewczuk M, et al. 8-Oxo-7,8-Dihydro-2'-Deoxyguanosine (8-oxodG) and 8-Hydroxy-2'-Deoxyguanosine (8-OHdG) as a Potential Biomarker for Gestational Diabetes Mellitus (GDM) Development. Molecules. 2020; 25(1).
- Nemtsova V, Bilovol O, Shalimova A. Vascular endothelial growth factor as a marker of endothelial dysfunction in poly- and comorbidity: focus on hypertension, type 2 diabetes mellitus and subclinical hypothyroidism. Arterial Hypertension. 2019; 23(2): 98–104.
- D'Agostino RB, Vasan RS, Pencina MJ, et al. General cardiovascular risk profile for use in primary care: the Framingham Heart Study. Circulation. 2008; 117(6): 743–753.
- Verweij L, Peters RJG, Scholte Op Reimer WJM, et al. Validation of the Systematic COronary Risk Evaluation — Older Persons (SCORE-OP) in the EPIC-Norfolk prospective population study. Int J Cardiol. 2019; 293: 226–230.