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Frequency of Gly972Arg polymorphism of the insulin receptor substrate 1 (IRS-1) gene in essential hypertension
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Abstract
Material and methods The study group comprised 115 nonobese (BMI 24.72 ± 1.93 kg/m2) patients, aged 27.48 ± 5.15 years, with essential and uncomplicated arterial hypertension, while 46 healthy persons aged 28.0 ± 4.39 years (BMI 22.6 ± 2.16 kg/m2) formed the control group. There were no cases of diabetes or dyslipidemia in the groups. A single sample of 5 ml venous blood was drawn and genomic DNA was isolated for genotyping with the polymerase chain reaction as described by Yamade et al.
Results The GA genotype was disclosed in 16 (13.9%) patients and in just 2 controls (4.3%), one of them (2.2%) being AA homozygote. The χ2 test did not disclose significant differences between both groups in the distribution of genotypes.
Conclusion The distribution of Gly972Arg polymorphism of the IRS-1 gene appears to be similar in hypertensives and normotensives suggesting that this polymorphism is not directly involved in the pathogenesis of arterial hypertension.
Abstract
Material and methods The study group comprised 115 nonobese (BMI 24.72 ± 1.93 kg/m2) patients, aged 27.48 ± 5.15 years, with essential and uncomplicated arterial hypertension, while 46 healthy persons aged 28.0 ± 4.39 years (BMI 22.6 ± 2.16 kg/m2) formed the control group. There were no cases of diabetes or dyslipidemia in the groups. A single sample of 5 ml venous blood was drawn and genomic DNA was isolated for genotyping with the polymerase chain reaction as described by Yamade et al.
Results The GA genotype was disclosed in 16 (13.9%) patients and in just 2 controls (4.3%), one of them (2.2%) being AA homozygote. The χ2 test did not disclose significant differences between both groups in the distribution of genotypes.
Conclusion The distribution of Gly972Arg polymorphism of the IRS-1 gene appears to be similar in hypertensives and normotensives suggesting that this polymorphism is not directly involved in the pathogenesis of arterial hypertension.
Keywords
arterial hypertension; insulin resistance; Gly972Arg polymorphism of the insulin receptor substrate-1 gene


Title
Frequency of Gly972Arg polymorphism of the insulin receptor substrate 1 (IRS-1) gene in essential hypertension
Journal
Issue
Article type
Original paper
Pages
174-179
Published online
2006-06-05
Page views
561
Article views/downloads
1206
Bibliographic record
Nadciśnienie tętnicze 2006;10(3):174-179.
Keywords
arterial hypertension
insulin resistance
Gly972Arg polymorphism of the insulin receptor substrate-1 gene
Authors
Joanna Dziwura
Agnieszka Bińczak-Kuleta
Krystyna Widecka