Online first
Review paper
Published online: 2025-02-27
From potential to practice: Overcoming the immaturity of iPSC-derived cardiomyocytes for regenerative medicine
Pubmed: 40066964
Abstract
Not available
References
- GBD 2013 Mortality and Causes of Death Collaborators. Global, regional, and national age–sex specific all-cause and cause-specific mortality for 240 causes of death, 1990–2013: A systematic analysis for the Global Burden of Disease Study 2013. Lancet. 2015; 385(9963): 117–171.
- Laflamme MA, Murry CE. Regenerating the heart. Nat Biotechnol. 2005; 23(7): 845–856.
- Tomasek JJ, Gabbiani G, Hinz B, et al. Myofibroblasts and mechano-regulation of connective tissue remodelling. Nat Rev Mol Cell Biol. 2002; 3(5): 349–363.
- Gill JK, Rehsia SK, Verma E, et al. Stem cell therapy for cardiac regeneration: past, present, and future. Can J Physiol Pharmacol. 2024; 102(3): 161–179.
- Huang Y, Wang T, López ME, et al. Recent advancements of human iPSC derived cardiomyocytes in drug screening and tissue regeneration. Microphysiol Syst. 2020; 4: 2.
- Fuerstenau-Sharp M, Zimmermann ME, Stark K, et al. Generation of highly purified human cardiomyocytes from peripheral blood mononuclear cell-derived induced pluripotent stem cells. PLoS One. 2015; 10(5): e0126596.
- Yin X, Li Q, Shu Y, et al. Exploiting urine-derived induced pluripotent stem cells for advancing precision medicine in cell therapy, disease modeling, and drug testing. J Biomed Sci. 2024; 31(1): 47.
- Hsueh YC, Pratt RE, Dzau VJ, et al. Novel method of differentiating human induced pluripotent stem cells to mature cardiomyocytes via Sfrp2. Sci Rep. 2023; 13(1): 3920.
- Breckwoldt K, Letuffe-Brenière D, Mannhardt I, et al. Differentiation of cardiomyocytes and generation of human engineered heart tissue. Nat Protoc. 2017; 12(6): 1177–1197.
- Lian X, Zhang J, Azarin SM, et al. Directed cardiomyocyte differentiation from human pluripotent stem cells by modulating Wnt/β-catenin signaling under fully defined conditions. Nat Protoc. 2013; 8(1): 162–175.
- Lian X, Hsiao C, Wilson G, et al. Robust cardiomyocyte differentiation from human pluripotent stem cells via temporal modulation of canonical Wnt signaling. Proc Natl Acad Sci U S A. 2012; 109(27): E1848–E1857.
- Wu P, Deng G, Sai X, et al. Maturation strategies and limitations of induced pluripotent stem cell-derived cardiomyocytes. Biosci Rep. 2021; 41(6): BSR20200833.
- Denning C, Borgdorff V, Crutchley J, et al. Cardiomyocytes from human pluripotent stem cells: From laboratory curiosity to industrial biomedical platform. Biochim Biophys Acta. 2016; 1863(7 Pt B): 1728–1748.
- Yang X, Pabon L, Murry CE. Engineering adolescence: Maturation of human pluripotent stem cell-derived cardiomyocytes. Circ Res. 2014; 114(3): 511–523.
- Lopaschuk GD, Jaswal JS. Energy metabolic phenotype of the cardiomyocyte during development, differentiation, and postnatal maturation. J Cardiovasc Pharmacol. 2010; 56(2): 130–140.
- Garbern JC, Lee RT. Mitochondria and metabolic transitions in cardiomyocytes: Lessons from development for stem cell-derived cardiomyocytes. Stem Cell Res Ther. 2021; 12(1): 177.
- Hinata Y, Kagawa Y, Kubo H, et al. Importance of beating rate control for the analysis of drug effects on contractility in human induced pluripotent stem cell-derived cardiomyocytes. J Pharmacol Toxicol Methods. 2022; 118: 107228.
- Hoekstra M, Mummery CL, Wilde AAM, et al. Induced pluripotent stem cell derived cardiomyocytes as models for cardiac arrhythmias. Front Physiol. 2012; 3: 346.
- Ma J, Guo L, Fiene SJ, et al. High purity human-induced pluripotent stem cell-derived cardiomyocytes: electrophysiological properties of action potentials and ionic currents. Am J Physiol Heart Circ Physiol. 2011; 301(5): H2006–H2017.
- Grancharova T, Gerbin KA, Rosenberg AB, et al. A comprehensive analysis of gene expression changes in a high replicate and open-source dataset of differentiating hiPSC-derived cardiomyocytes. Sci Rep. 2021; 11(1): 15845.
- MacGrogan D, Münch J, de la Pompa JL. Notch and interacting signalling pathways in cardiac development, disease, and regeneration. Nat Rev Cardiol. 2018; 15(11): 685–704.
- Zhou Q, Li L, Zhao B, et al. The hippo pathway in heart development, regeneration, and diseases. Circ Res. 2015; 116(8): 1431–1447.
- Karbassi E, Fenix A, Marchiano S, et al. Cardiomyocyte maturation: Advances in knowledge and implications for regenerative medicine. Nat Rev Cardiol. 2020; 17(6): 341–359.
- Yang H, Yang Y, Kiskin F, et al. Recent advances in regulating the proliferation or maturation of human-induced pluripotent stem cell-derived cardiomyocytes. Stem Cell Res Ther. 2023; 14(1): 228.
- Qiu XX, Liu Y, Zhang YF, et al. Rapamycin and CHIR99021 coordinate robust cardiomyocyte differentiation from human pluripotent stem cells via reducing p53-dependent apoptosis. J Am Heart Assoc. 2017; 6(10): e005295.
- Fan C, Tang Y, Zhao M, et al. CHIR99021 and fibroblast growth factor 1 enhance the regenerative potency of human cardiac muscle patch after myocardial infarction in mice. J Mol Cell Cardiol. 2020; 141: 1–10.
- Wu KH, Wang SuY, Xiao QR, et al. Small-molecule-based generation of functional cardiomyocytes from human umbilical cord-derived induced pluripotent stem cells. J Cell Biochem. 2019; 120(2): 1318–1327.
- Yang X, Rodriguez ML, Leonard A, et al. Fatty acids enhance the maturation of cardiomyocytes derived from human pluripotent stem cells. Stem Cell Reports. 2019; 13(4): 657–668.
- Horikoshi Y, Yan Y, Terashvili M, et al. Fatty acid-treated induced pluripotent stem cell-derived human cardiomyocytes exhibit adult cardiomyocyte-like energy metabolism phenotypes. Cells. 2019; 8(9): 1095.
- Lin B, Lin X, Stachel M, et al. Culture in glucose-depleted medium supplemented with fatty acid and 3,3',5-triiodo-l-thyronine facilitates purification and maturation of human pluripotent stem cell-derived cardiomyocytes. Front Endocrinol (Lausanne). 2017; 8: 253.
- Shimoji K, Yuasa S, Onizuka T, et al. G-CSF promotes the proliferation of developing cardiomyocytes in vivo and in derivation from ESCs and iPSCs. Cell Stem Cell. 2010; 6(3): 227–237.
- Zwi-Dantsis L, Mizrahi I, Arbel G, et al. Scalable production of cardiomyocytes derived from c-Myc free induced pluripotent stem cells. Tissue Eng Part A. 2011; 17(7-8): 1027–1037.
- Wobus AM, Wallukat G, Hescheler J. Pluripotent mouse embryonic stem cells are able to differentiate into cardiomyocytes expressing chronotropic responses to adrenergic and cholinergic agents and Ca2+ channel blockers. Differentiation. 1991; 48(3): 173–182.
- Mauritz C, Schwanke K, Reppel M, et al. Generation of functional murine cardiac myocytes from induced pluripotent stem cells. Circulation. 2008; 118(5): 507–517.
- Bufi S, Santoro R. Three-dimensional iPSC-based in vitro cardiac models for biomedical and pharmaceutical research applications. Int J Mol Sci. 2024; 25(19): 10690.
- Sacchetto C, Vitiello L, de Windt LJ, et al. Modeling cardiovascular diseases with hiPSC-derived cardiomyocytes in 2D and 3D cultures. Int J Mol Sci. 2020; 21(9): 3404.
- Ronaldson-Bouchard K, Yeager K, Teles D, et al. Engineering of human cardiac muscle electromechanically matured to an adult-like phenotype. Nat Protoc. 2019; 14(10): 2781–2817.
- Scuderi GJ, Butcher J. Naturally engineered maturation of cardiomyocytes. Front Cell Dev Biol. 2017; 5: 50.
- Leucker TM, Bienengraeber M, Muravyeva M, et al. Endothelial-cardiomyocyte crosstalk enhances pharmacological cardioprotection. J Mol Cell Cardiol. 2011; 51(5): 803–811.
- Yoshida S, Miyagawa S, Fukushima S, et al. Maturation of human induced pluripotent stem cell-derived cardiomyocytes by soluble factors from human mesenchymal stem cells. Mol Ther. 2018; 26(11): 2681–2695.
- Kistamás K, Lamberto F, Vaiciuleviciute R, et al. The current state of realistic heart models for disease modelling and cardiotoxicity. Int J Mol Sci. 2024; 25(17): 9186.
- Abulaiti M, Yalikun Y, Murata K, et al. Establishment of a heart-on-a-chip microdevice based on human iPS cells for the evaluation of human heart tissue function. Sci Rep. 2020; 10(1): 19201.
- Tan Y, Coyle RC, Barrs RW, et al. Nanowired human cardiac organoid transplantation enables highly efficient and effective recovery of infarcted hearts. Sci Adv. 2023; 9(31): eadf2898.
- Varzideh F, Mone P, Santulli G. Bioengineering strategies to create 3D cardiac constructs from human induced pluripotent stem cells. Bioengineering (Basel). 2022; 9(4): 168.
- Kitsuka T, Itoh M, Amamoto S, et al. 2-Cl-C.OXT-A stimulates contraction through the suppression of phosphodiesterase activity in human induced pluripotent stem cell-derived cardiac organoids. PLoS One. 2019; 14(7): e0213114.
- Veldhuizen J, Chavan R, Moghadas B, et al. Cardiac ischemia on-a-chip to investigate cellular and molecular response of myocardial tissue under hypoxia. Biomaterials. 2022; 281: 121336.
- Lai BF, Lu RX, Davenport Huyer L, et al. A well plate-based multiplexed platform for incorporation of organoids into an organ-on-a-chip system with a perfusable vasculature. Nat Protoc. 2021; 16(4): 2158–2189.
- Zhao Y, Rafatian N, Wang EY, et al. Engineering microenvironment for human cardiac tissue assembly in heart-on-a-chip platform. Matrix Biol. 2020; 85-86: 189–204.
- Zhao Y, Rafatian N, Feric NT, et al. A platform for generation of chamber-specific cardiac tissues and disease modeling. Cell. 2019; 176(4): 913–927.e18.
- Amano Y, Nishiguchi A, Matsusaki M, et al. Development of vascularized iPSC derived 3D-cardiomyocyte tissues by filtration Layer-by-Layer technique and their application for pharmaceutical assays. Acta Biomater. 2016; 33: 110–121.
- Tadano K, Miyagawa S, Takeda M, et al. Cardiotoxicity assessment using 3D vascularized cardiac tissue consisting of human iPSC-derived cardiomyocytes and fibroblasts. Mol Ther Methods Clin Dev. 2021; 22: 338–349.
- Schaefer JA, Guzman PA, Riemenschneider SB, et al. A cardiac patch from aligned microvessel and cardiomyocyte patches. J Tissue Eng Regen Med. 2018; 12(2): 546–556.
- Iwoń Z, Krogulec E, Kierlańczyk A, et al. Hypoxia and re-oxygenation effects on human cardiomyocytes cultured on polycaprolactone and polyurethane nanofibrous mats. J Biol Eng. 2024; 18(1): 37.
- Iwoń Z, Krogulec E, Kierlańczyk A, et al. Improving rodents and humans cardiac cell maturitythrough polycaprolactone and polyurethane nanofibers. Biomed Mater. 2024; 19(2).
- Nikolova MP, Chavali MS. Recent advances in biomaterials for 3D scaffolds: A review. Bioact Mater. 2019; 4: 271–292.
- Iwoń Z, Krogulec E, Tarnowska I, et al. Maturation of human cardiomyocytes derived from induced pluripotent stem cells (iPSC-CMs) on polycaprolactone and polyurethane nanofibrous mats. Sci Rep. 2024; 14(1): 12975.
- Zhang M, Xu Y, Chen Y, et al. Three-dimensional poly-(ε-caprolactone) nanofibrous scaffolds promote the maturation of human pluripotent stem cells-induced cardiomyocytes. Front Cell Dev Biol. 2022; 10: 875278.
- Silbernagel N, Körner A, Balitzki J, et al. Shaping the heart: Structural and functional maturation of iPSC-cardiomyocytes in 3D-micro-scaffolds. Biomaterials. 2020; 227: 119551.
- LaBarge W, Mattappally S, Kannappan R, et al. Maturation of three-dimensional, hiPSC-derived cardiomyocyte spheroids utilizing cyclic, uniaxial stretch and electrical stimulation. PLoS One. 2019; 14(7): e0219442.
- Ruan JL, Tulloch NL, Saiget M, et al. Mechanical stress promotes maturation of human myocardium from pluripotent stem cell-derived progenitors. Stem Cells. 2015; 33(7): 2148–2157.
- Zhang X, Ye L, Xu H, et al. NRF2 is required for structural and metabolic maturation of human induced pluripotent stem cell-derived ardiomyocytes. Stem Cell Res Ther. 2021; 12(1): 208.
- Mihic A, Li J, Miyagi Y, et al. The effect of cyclic stretch on maturation and 3D tissue formation of human embryonic stem cell-derived cardiomyocytes. Biomaterials. 2014; 35(9): 2798–2808.
- Tulloch NL, Muskheli V, Razumova MV, et al. Growth of engineered human myocardium with mechanical loading and vascular coculture. Circ Res. 2011; 109(1): 47–59.
- Hernández D, Millard R, Sivakumaran P, et al. Electrical stimulation promotes cardiac differentiation of human induced pluripotent stem cells. Stem Cells Int. 2016; 2016: 1718041.
- Maihemuti W, Murata K, Abulaiti M, et al. Simultaneous electro-dynamic stimulation accelerates maturation of engineered cardiac tissues generated by human iPS cells. Biochem Biophys Res Commun. 2024; 733: 150605.
- Masuda A, Kurashina Y, Tani H, et al. Maturation of human iPSC-derived cardiac microfiber with electrical stimulation device. Adv Healthc Mater. 2024; 13(27): e2303477.
- Kroll K, Chabria M, Wang K, et al. Electro-mechanical conditioning of human iPSC-derived cardiomyocytes for translational research. Prog Biophys Mol Biol. 2017; 130(Pt B): 212–222.
- Zhao M, Nakada Y, Wei Y, et al. Cyclin D2 overexpression enhances the efficacy of human induced pluripotent stem cell-derived cardiomyocytes for myocardial repair in a swine model of myocardial infarction. Circulation. 2021; 144(3): 210–228.
- Zhu W, Zhao M, Mattapally S, et al. CCND2 overexpression enhances the regenerative potency of human induced pluripotent stem cell-derived cardiomyocytes: remuscularization of injured ventricle. Circ Res. 2018; 122(1): 88–96.
- Lou X, Zhao M, Fan C, et al. N-cadherin overexpression enhances the reparative potency of human-induced pluripotent stem cell-derived cardiac myocytes in infarcted mouse hearts. Cardiovasc Res. 2020; 116(3): 671–685.
- Kumar A, He S, Mali P. Systematic discovery of transcription factors that improve hPSC-derived cardiomyocyte maturation via temporal analysis of bioengineered cardiac tissues. APL Bioeng. 2023; 7(2): 026109.
- Zhou J, Cui B, Wang X, et al. Overexpression of KCNJ2 enhances maturation of human-induced pluripotent stem cell-derived cardiomyocytes. Stem Cell Res Ther. 2023; 14(1): 92.
- Lieu DK, Fu JD, Chiamvimonvat N, et al. Mechanism-based facilitated maturation of human pluripotent stem cell-derived cardiomyocytes. Circ Arrhythm Electrophysiol. 2013; 6(1): 191–201.
- Kim YJ, Tamadon A, Kim YY, et al. Epigenetic regulation of cardiomyocyte differentiation from embryonic and induced pluripotent stem cells. Int J Mol Sci. 2021; 22(16): 8599.
- Scesa G, Adami R, Bottai D. iPSC preparation and epigenetic memory: Does the tissue origin matter? Cells. 2021; 10(6): 1470.
- Kim K, Zhao R, Doi A, et al. Epigenetic memory in induced pluripotent stem cells. Nature. 2010; 467(7313): 285–290.
- Diez-Cuñado M, Wei Ke, Bushway PJ, et al. miRNAs that induce human cardiomyocyte proliferation converge on the hippo pathway. Cell Rep. 2018; 23(7): 2168–2174.
- Bian W, Chen W, Nguyen T, et al. miR-199a overexpression enhances the potency of human induced-pluripotent stem-cell-derived cardiomyocytes for myocardial repair. Front Pharmacol. 2021; 12: 673621.
- Xu F, Yang J, Shang J, et al. MicroRNA-302d promotes the proliferation of human pluripotent stem cell-derived cardiomyocytes by inhibiting in the Hippo pathway. Clin Sci (Lond). 2019; 133(13): 1387–1399.
- Miklas JW, Clark E, Levy S, et al. TFPa/HADHA is required for fatty acid beta-oxidation and cardiolipin re-modeling in human cardiomyocytes. Nat Commun. 2019; 10(1): 4671.
- Kuppusamy KT, Jones DC, Sperber H, et al. Let-7 family of microRNA is required for maturation and adult-like metabolism in stem cell-derived cardiomyocytes. Proc Natl Acad Sci U S A. 2015; 112(21): E2785–E2794.
- Fu JD, Rushing SN, Lieu DK, et al. Distinct roles of microRNA-1 and -499 in ventricular specification and functional maturation of human embryonic stem cell-derived cardiomyocytes. PLoS One. 2011; 6(11): e27417.
- Wang Z, Cui M, Shah AM, et al. Mechanistic basis of neonatal heart regeneration revealed by transcriptome and histone modification profiling. Proc Natl Acad Sci U S A. 2019; 116(37): 18455–18465.
- Gilsbach R, Schwaderer M, Preissl S, et al. Distinct epigenetic programs regulate cardiac myocyte development and disease in the human heart in vivo. Nat Commun. 2018; 9(1): 391.
- Biermann M, Cai W, Lang Di, et al. Epigenetic priming of human pluripotent stem cell-derived cardiac progenitor cells accelerates cardiomyocyte maturation. Stem Cells. 2019; 37(7): 910–923.
- Deogharia M, Venegas-Zamora L, Agrawal A, et al. Histone demethylase KDM5 regulates cardiomyocyte maturation by promoting fatty acid oxidation, oxidative phosphorylation, and myofibrillar organization. Cardiovasc Res. 2024; 120(6): 630–643.
- Li C, Zhang Y, Shen J, et al. Cfp1 controls cardiomyocyte maturation by modifying histone H3K4me3 of structural, metabolic, and contractile related genes. Adv Sci (Weinh). 2024; 11(11): e2305992.
- Han Z, Wang X, Xu Z, et al. ALKBH5 regulates cardiomyocyte proliferation and heart regeneration by demethylating the mRNA of YTHDF1. Theranostics. 2021; 11(6): 3000–3016.
- Dias TP, Pinto SN, Santos JI, et al. Biophysical study of human induced Pluripotent Stem Cell-Derived cardiomyocyte structural maturation during long-term culture. Biochem Biophys Res Commun. 2018; 499(3): 611–617.
- Lundy SD, Zhu WZ, Regnier M, et al. Structural and functional maturation of cardiomyocytes derived from human pluripotent stem cells. Stem Cells Dev. 2013; 22(14): 1991–2002.
- Ebert A, Joshi AU, Andorf S, et al. Proteasome-Dependent regulation of distinct metabolic states during long-term culture of human iPSC-derived cardiomyocytes. Circ Res. 2019; 125(1): 90–103.
- Lewandowski J, Rozwadowska N, Kolanowski TJ, et al. The impact of in vitro cell culture duration on the maturation of human cardiomyocytes derived from induced pluripotent stem cells of myogenic origin. Cell Transplant. 2018; 27(7): 1047–1067.
- Kamakura T, Makiyama T, Sasaki K, et al. Ultrastructural maturation of human-induced pluripotent stem cell-derived cardiomyocytes in a long-term culture. Circ J. 2013; 77(5): 1307–1314.
- Kumar N, Dougherty JA, Manring HR, et al. Assessment of temporal functional changes and miRNA profiling of human iPSC-derived cardiomyocytes. Sci Rep. 2019; 9(1): 13188.
- Fukushima H, Yoshioka M, Kawatou M, et al. Specific induction and long-term maintenance of high purity ventricular cardiomyocytes from human induced pluripotent stem cells. PLoS One. 2020; 15(11): e0241287.
- Seibertz F, Sutanto H, Dülk R, et al. Electrophysiological and calcium-handling development during long-term culture of human-induced pluripotent stem cell-derived cardiomyocytes. Basic Res Cardiol. 2023; 118(1): 14.
- Li Q, Wang J, Wu Q, et al. Perspective on human pluripotent stem cell-derived cardiomyocytes in heart disease modeling and repair. Stem Cells Transl Med. 2020; 9(10): 1121–1128.
- Campuzano O, Beltrán-Alvarez P, Iglesias A, et al. Genetics and cardiac channelopathies. Genet Med. 2010; 12(5): 260–267.
- Brandão KO, van den Brink L, Miller DC, et al. Isogenic sets of hiPSC-CMs harboring distinct KCNH2 mutations differ functionally and in susceptibility to drug-induced arrhythmias. Stem Cell Reports. 2020; 15(5): 1127–1139.
- Takaki T, Inagaki A, Chonabayashi K, et al. Optical recording of action potentials in human induced pluripotent stem cell-derived cardiac single cells and monolayers generated from long QT syndrome type 1 patients. Stem Cells International. 2019; 2019: 7532657.
- Crotti L, Neves R, Dagradi F, et al. From patient-specific induced pluripotent stem cells to clinical translation in long QT syndrome type 2. Eur Heart J. 2019; 40(23): 1832–1836.
- Mehta A, Ramachandra CJA, Singh P, et al. Identification of a targeted and testable antiarrhythmic therapy for long-QT syndrome type 2 using a patient-specific cellular model. Eur Heart J. 2018; 39(16): 1446–1455.
- Ma D, Wei H, Lu J, et al. Characterization of a novel KCNQ1 mutation for type 1 long QT syndrome and assessment of the therapeutic potential of a novel IKs activator using patient-specific induced pluripotent stem cell-derived cardiomyocytes. Stem Cell Res Ther. 2015; 6(1): 39.
- Wang Y, Liang P, Lan F, et al. Genome editing of isogenic human induced pluripotent stem cells recapitulates long QT phenotype for drug testing. J Am Coll Cardiol. 2014; 64(5): 451–459.
- Itzhaki I, Maizels L, Huber I, et al. Modelling the long QT syndrome with induced pluripotent stem cells. Nature. 2011; 471(7337): 225–229.
- Moretti A, Bellin M, Welling A, et al. Patient-specific induced pluripotent stem-cell models for long-QT syndrome. N Engl J Med. 2010; 363(15): 1397–1409.
- Stutzman MJ, Kim CS, Tester DJ, et al. Characterization of N-terminal RYR2 variants outside CPVT1 hotspot regions using patient iPSCs reveal pathogenesis and therapeutic potential. Stem Cell Reports. 2022; 17(9): 2023–2036.
- Acimovic I, Refaat MM, Moreau A, et al. Post-translational modifications and diastolic calcium leak associated to the novel RyR2-D3638A mutation lead to CPVT in patient-specific hiPSC-derived cardiomyocytes. J Clin Med. 2018; 7(11): 423.
- Fatima A, Xu G, Shao K, et al. In vitro modeling of ryanodine receptor 2 dysfunction using human induced pluripotent stem cells. Cell Physiol Biochem. 2011; 28(4): 579–592.
- Zhong R, Schimanski T, Zhang F, et al. A preclinical study on Brugada syndrome with a CACNB2 variant using human cardiomyocytes from induced pluripotent stem cells. Int J Mol Sci. 2022; 23(15).
- Zhu Y, Wang L, Cui C, et al. Pathogenesis and drug response of iPSC-derived cardiomyocytes from two Brugada syndrome patients with different Nav1.5-subunit mutations. J Biomed Res. 2021; 35(5): 395–407.
- Li W, Stauske M, Luo X, et al. Disease phenotypes and mechanisms of iPSC-derived cardiomyocytes from brugada syndrome patients with a loss-of-function SCN5A mutation. Front Cell Dev Biol. 2020; 8: 592893.
- Wexler RK, Elton T, Pleister A, et al. Cardiomyopathy: An overview. Am Fam Physician. 2009; 79(9): 778–784.
- Mori H, Xu D, Shimoda Y, et al. Metabolic remodeling and calcium handling abnormality in induced pluripotent stem cell-derived cardiomyocytes in dilated phase of hypertrophic cardiomyopathy with MYBPC3 frameshift mutation. Sci Rep. 2024; 14(1): 15422.
- Escribá R, Larrañaga-Moreira JM, Richaud-Patin Y, et al. iPSC-based modeling of variable clinical presentation in hypertrophic cardiomyopathy. Circ Res. 2023; 133(2): 108–119.
- Korover N, Etzion S, Cherniak A, et al. Functional defects in hiPSCs-derived cardiomyocytes from patients with a PLEKHM2-mutation associated with dilated cardiomyopathy and left ventricular non-compaction. Biol Res. 2023; 56(1): 34.
- Dai Y, Amenov A, Ignatyeva N, et al. Troponin destabilization impairs sarcomere-cytoskeleton interactions in iPSC-derived cardiomyocytes from dilated cardiomyopathy patients. Sci Rep. 2020; 10(1): 209.
- Caspi O, Huber I, Gepstein A, et al. Modeling of arrhythmogenic right ventricular cardiomyopathy with human induced pluripotent stem cells. Circ Cardiovasc Genet. 2013; 6(6): 557–568.
- Prondzynski M, Bortolin RH, Berkson P, et al. Efficient and reproducible generation of human iPSC-derived cardiomyocytes using a stirred bioreactor. bioRxiv. 2024.
- Lin Y, Sato N, Hong S, et al. Long-term engraftment and maturation of autologous iPSC-derived cardiomyocytes in two rhesus macaques. Cell Stem Cell. 2024; 31(7): 974–988.e5.
- Andrysiak K, Stępniewski J, Dulak J. Human-induced pluripotent stem cell-derived cardiomyocytes, 3D cardiac structures, and heart-on-a-chip as tools for drug research. Pflugers Arch. 2021; 473(7): 1061–1085.
- Yadid M, Oved H, Silberman E, et al. Bioengineering approaches to treat the failing heart: From cell biology to 3D printing. Nat Rev Cardiol. 2022; 19(2): 83–99.
