Analysis of plasma myeloperoxidase levels and functional gene –463G>A and –129G>A polymorphisms with early onset of coronary artery disease in South Indian population

Streszczenie

Introduction. The present investigation is pursued to study the possible association of –463G>A and –129G>A polymorphism in MPO gene and assessment of plasma MPO levels with the risk of developing coronary artery disease.
Material and methods. A total of 200 angiographically documented CAD patients and 200 age, gender ethnicity matched healthy controls were recruited for the study. Plasma MPO levels were assessed using enzyme-linked immunosorbent assay (ELISA) kit and genotypes were determined by PCR-RFLP technique.
Results. The MPO levels were found to be significantly increased in CAD patients when compared with controls (p < 0.04) but there were no significant effect of –463G>A gene polymorphism on MPO levels. A significant association of –463G>A polymorphism was observed with coronary artery disease. The frequency of recessive genotype “AA” at –463 promoter site was considerably lesser in patients (4%) relative to controls (11%) (odds ratio [OR] = 0.3371, 95% confidence interval [CI] 0.1463–0.7766, p = 0.012). However we did not find significant association of –129G>A polymorphism with CAD. Additionally, haplotype analysis revealed that single nucleotide polymorphisms (SNP) 1 of AA genotype and SNP 2 of GG genotype showed significant protective effect with disease (OR = 0.64; 95% CI [0.42–0.96], p = 0.032).
Conclusion. The results revealed that –463G>A polymorphism in the MPO gene lowers the CAD related condition in patients by down regulating serum MPO concentration, which is known to aggravate the atherosclerotic events observed in CAD.