open access

Vol 11, No 4 (2016)
Original Papers
Published online: 2016-09-13
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Analysis of plasma myeloperoxidase levels and functional gene –463G > A and –129G > A polymorphisms with early onset of coronary artery disease in South Indian population

Sailaja Maddhuri, Priyanka Pallapollu, Srinivas Bandaru, Gudlla Suresh, Amaresh Rao Mallempati, Akka Jyothy, Hema Prasad Mundluru
DOI: 10.5603/FC.2016.0047
·
Folia Cardiologica 2016;11(4):272-278.

open access

Vol 11, No 4 (2016)
Original Papers
Published online: 2016-09-13

Abstract

Introduction. The present investigation is pursued to study the possible association of –463G>A and –129G>A polymorphism in MPO gene and assessment of plasma MPO levels with the risk of developing coronary artery disease.
Material and methods. A total of 200 angiographically documented CAD patients and 200 age, gender ethnicity matched healthy controls were recruited for the study. Plasma MPO levels were assessed using enzyme-linked immunosorbent assay (ELISA) kit and genotypes were determined by PCR-RFLP technique.
Results. The MPO levels were found to be significantly increased in CAD patients when compared with controls (p < 0.04) but there were no significant effect of –463G>A gene polymorphism on MPO levels. A significant association of –463G>A polymorphism was observed with coronary artery disease. The frequency of recessive genotype “AA” at –463 promoter site was considerably lesser in patients (4%) relative to controls (11%) (odds ratio [OR] = 0.3371, 95% confidence interval [CI] 0.1463–0.7766, p = 0.012). However we did not find significant association of –129G>A polymorphism with CAD. Additionally, haplotype analysis revealed that single nucleotide polymorphisms (SNP) 1 of AA genotype and SNP 2 of GG genotype showed significant protective effect with disease (OR = 0.64; 95% CI [0.42–0.96], p = 0.032).
Conclusion. The results revealed that –463G>A polymorphism in the MPO gene lowers the CAD related condition in patients by down regulating serum MPO concentration, which is known to aggravate the atherosclerotic events observed in CAD.

Abstract

Introduction. The present investigation is pursued to study the possible association of –463G>A and –129G>A polymorphism in MPO gene and assessment of plasma MPO levels with the risk of developing coronary artery disease.
Material and methods. A total of 200 angiographically documented CAD patients and 200 age, gender ethnicity matched healthy controls were recruited for the study. Plasma MPO levels were assessed using enzyme-linked immunosorbent assay (ELISA) kit and genotypes were determined by PCR-RFLP technique.
Results. The MPO levels were found to be significantly increased in CAD patients when compared with controls (p < 0.04) but there were no significant effect of –463G>A gene polymorphism on MPO levels. A significant association of –463G>A polymorphism was observed with coronary artery disease. The frequency of recessive genotype “AA” at –463 promoter site was considerably lesser in patients (4%) relative to controls (11%) (odds ratio [OR] = 0.3371, 95% confidence interval [CI] 0.1463–0.7766, p = 0.012). However we did not find significant association of –129G>A polymorphism with CAD. Additionally, haplotype analysis revealed that single nucleotide polymorphisms (SNP) 1 of AA genotype and SNP 2 of GG genotype showed significant protective effect with disease (OR = 0.64; 95% CI [0.42–0.96], p = 0.032).
Conclusion. The results revealed that –463G>A polymorphism in the MPO gene lowers the CAD related condition in patients by down regulating serum MPO concentration, which is known to aggravate the atherosclerotic events observed in CAD.

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Keywords

early onset of CAD, myeloperoxidase, gene polymorphism, Atheros

About this article
Title

Analysis of plasma myeloperoxidase levels and functional gene –463G>A and –129G>A polymorphisms with early onset of coronary artery disease in South Indian population

Journal

Folia Cardiologica

Issue

Vol 11, No 4 (2016)

Pages

272-278

Published online

2016-09-13

DOI

10.5603/FC.2016.0047

Bibliographic record

Folia Cardiologica 2016;11(4):272-278.

Keywords

early onset of CAD
myeloperoxidase
gene polymorphism
Atheros

Authors

Sailaja Maddhuri
Priyanka Pallapollu
Srinivas Bandaru
Gudlla Suresh
Amaresh Rao Mallempati
Akka Jyothy
Hema Prasad Mundluru

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