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Serum parathyroid hormone concentrations measured by chemiluminescence and electrochemiluminescence methods — are the results comparable in haemodialysis patients with chronic kidney disease?
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Abstract
Introduction: Secondary hyperparathyroidism (sHPT) is one of the most common abnormalities found in patients with chronic kidney disease (CKD). Measurement of serum PTH concentrations is crucial in diagnosis and treatment of sHPT. Different methods of serum PTH measurement may provide diverse results. This may have a significant impact on the therapeutic approach if under- or over-diagnosis of sHPT occurs. The aim of this study was to compare the results of serum PTH concentrations measured with two commonly used methods — chemiluminescence (CHL) and electrochemiluminescence (ECL).
Material and methods: Seventy-seven haemodialysis patients with CKD were enrolled into the study. Blood samples were collected before haemodialysis, in the middle of the week. In all patients, serum PTH concentrations were measured using two methods: CHL and ECL.
Results: Serum PTH concentration measured with CHL was significantly higher than that assessed with ECL: 455 pg/mL (352–559) pg/mL vs. 383 pg/mL (243–523) pg/mL; p < 0.0001. Six patients from the studied cohort were treated with cinacalcet. In these patients, the serum PTH concentration was also significantly higher when measured with CHL than with ECL: 755 pg/mL (294–1216) pg/mL and 607 pg/mL (199–1015 pg/mL); p = 0.027, respectively). In three cases serum PTH concentration assessed with CHL method exceeded 300 pg/mL, whereas when measured with ECL it was below 300 pg/mL. Lower serum PTH concentrations could give the rationale to lower cinacalcet dose or to stop such treatment.
Conclusions: 1. Serum PTH concentrations in haemodialysis patients with CKD measured by CHL and ECL methods differ significantly. 2. The choice of method for measurement of serum PTH concentration in these patients may have important clinical implications. (Endokrynol Pol 2015; 66 (3): 219–223)
Abstract
Introduction: Secondary hyperparathyroidism (sHPT) is one of the most common abnormalities found in patients with chronic kidney disease (CKD). Measurement of serum PTH concentrations is crucial in diagnosis and treatment of sHPT. Different methods of serum PTH measurement may provide diverse results. This may have a significant impact on the therapeutic approach if under- or over-diagnosis of sHPT occurs. The aim of this study was to compare the results of serum PTH concentrations measured with two commonly used methods — chemiluminescence (CHL) and electrochemiluminescence (ECL).
Material and methods: Seventy-seven haemodialysis patients with CKD were enrolled into the study. Blood samples were collected before haemodialysis, in the middle of the week. In all patients, serum PTH concentrations were measured using two methods: CHL and ECL.
Results: Serum PTH concentration measured with CHL was significantly higher than that assessed with ECL: 455 pg/mL (352–559) pg/mL vs. 383 pg/mL (243–523) pg/mL; p < 0.0001. Six patients from the studied cohort were treated with cinacalcet. In these patients, the serum PTH concentration was also significantly higher when measured with CHL than with ECL: 755 pg/mL (294–1216) pg/mL and 607 pg/mL (199–1015 pg/mL); p = 0.027, respectively). In three cases serum PTH concentration assessed with CHL method exceeded 300 pg/mL, whereas when measured with ECL it was below 300 pg/mL. Lower serum PTH concentrations could give the rationale to lower cinacalcet dose or to stop such treatment.
Conclusions: 1. Serum PTH concentrations in haemodialysis patients with CKD measured by CHL and ECL methods differ significantly. 2. The choice of method for measurement of serum PTH concentration in these patients may have important clinical implications. (Endokrynol Pol 2015; 66 (3): 219–223)
Keywords
parathyroid hormone; secondary hyperparathyroidism; haemodialysis; chemiluminescence; electrochemiluminescence; cinacalcet
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About this articleTitle
Serum parathyroid hormone concentrations measured by chemiluminescence and electrochemiluminescence methods — are the results comparable in haemodialysis patients with chronic kidney disease?
Journal
Issue
Article type
Original paper
Pages
219-223
Published online
2015-07-01
Page views
1542
Article views/downloads
2149
DOI
10.5603/EP.2015.0028
Pubmed
Bibliographic record
Endokrynol Pol 2015;66(3):219-223.
Keywords
parathyroid hormone
secondary hyperparathyroidism
haemodialysis
chemiluminescence
electrochemiluminescence
cinacalcet
Authors
Piotr Kuczera
Agata Maszczyk
Ewelina Machura
Ewa Kurzak
Marcin Adamczak
Andrzej Więcek
