open access

Vol 57, Supp. A (2006)
Original papers
Published online: 2006-09-25
Submitted: 2013-02-15
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Analysis of expression of LGALS3BP gene in thyroid tissues and peripheral blood lymphocytes in patients with papillary thyroid cancer

Krzysztof Kaliszewski, Tadeusz Łukieńczuk, Tadeusz Dobosz, Marta Rzeszutko, Anna Sadakierska-Chudy

open access

Vol 57, Supp. A (2006)
Original papers
Published online: 2006-09-25
Submitted: 2013-02-15

Abstract

Introduction: Galectin-3 (Gal-3), coding by LGALS3BP gene, is a protein of the lectin family that has been associated with neoplastic processes and seems to play an important role in a variety of cell biological processes. In the thyroid gland, the high expression of this protein has been described in differentiated carcinomas, especially in papillary thyroid cancer (PTC).
Aim: Analysis of Gal-3 protein expression in PTC and nodular goiters; investigation of Gal-3 mRNA expression in peripheral blood lymphocytes (PBL) in patients with PTC and nodular goiter; analysis of correlation between Gal-3 protein expression in PTC and Gal-3 mRNA in PBL of the same patient.
Material and methods: Gal-3 protein was evaluated by immunohistochemisty in benign (27 multinodular goiters) and malignant (30 papillary carcinomas) thyroid tissues and galectin-3 mRNA expression by real-time PCR in peripheral blood lymphocytes (PBL) from 90 patients with multinodular goiter (n = 27), papillary carcinoma (n = 30) and healthy controls (n = 33).
Results: In PTC we observed increased expression of Gal-3 protein (all 30 cases) in cytoplasm, nucleus and cell membranes of cancer cells. 23 of 27 benign thyroid nodular goiters were negative for Gal-3 expression. In all examined blood samples we observed higher LGALS3BP gene expression than GAPDH (house keeping gene) with no difference between both groups, without relation to the Gal-3 expression in PTC.
Conclusions: There is no difference in Gal-3 expression in peripheral blood lymphocytes in patients with papillary thyroid cancer in relation to nodular goiter.

Abstract

Introduction: Galectin-3 (Gal-3), coding by LGALS3BP gene, is a protein of the lectin family that has been associated with neoplastic processes and seems to play an important role in a variety of cell biological processes. In the thyroid gland, the high expression of this protein has been described in differentiated carcinomas, especially in papillary thyroid cancer (PTC).
Aim: Analysis of Gal-3 protein expression in PTC and nodular goiters; investigation of Gal-3 mRNA expression in peripheral blood lymphocytes (PBL) in patients with PTC and nodular goiter; analysis of correlation between Gal-3 protein expression in PTC and Gal-3 mRNA in PBL of the same patient.
Material and methods: Gal-3 protein was evaluated by immunohistochemisty in benign (27 multinodular goiters) and malignant (30 papillary carcinomas) thyroid tissues and galectin-3 mRNA expression by real-time PCR in peripheral blood lymphocytes (PBL) from 90 patients with multinodular goiter (n = 27), papillary carcinoma (n = 30) and healthy controls (n = 33).
Results: In PTC we observed increased expression of Gal-3 protein (all 30 cases) in cytoplasm, nucleus and cell membranes of cancer cells. 23 of 27 benign thyroid nodular goiters were negative for Gal-3 expression. In all examined blood samples we observed higher LGALS3BP gene expression than GAPDH (house keeping gene) with no difference between both groups, without relation to the Gal-3 expression in PTC.
Conclusions: There is no difference in Gal-3 expression in peripheral blood lymphocytes in patients with papillary thyroid cancer in relation to nodular goiter.
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Keywords

papillary thyroid cancer; immunological system; lymphocytes; galectin-3; LGALS3BP gene; real time polymerase chain reaction

About this article
Title

Analysis of expression of LGALS3BP gene in thyroid tissues and peripheral blood lymphocytes in patients with papillary thyroid cancer

Journal

Endokrynologia Polska

Issue

Vol 57, Supp. A (2006)

Pages

38-44

Published online

2006-09-25

Keywords

papillary thyroid cancer
immunological system
lymphocytes
galectin-3
LGALS3BP gene
real time polymerase chain reaction

Authors

Krzysztof Kaliszewski
Tadeusz Łukieńczuk
Tadeusz Dobosz
Marta Rzeszutko
Anna Sadakierska-Chudy

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