Vol 8, No 2 (2004)
Review paper
Published online: 2004-04-06
G protein β3 subunit gene polymorphism and arterial blood pressure, indices of arterial structure and function
Nadciśnienie tętnicze 2004;8(2):119-131.
Abstract
There is some evidence that GNB3 C825T may be associated with metabolic syndrome phenotypes including essential
hypertension. We investigated whether blood pressure or indices of arterial structure and function are associated
with GNB3 polymorphism in Polish population.
We studied 86 nuclear families - 142 parents (age 50.3 ± 5.0 years) and 184 offspring (age 24.0 ± 4.7 years) enrolled in the population-based study in Cracow. Each subject underwent the 24-hr ambulatory blood pressure monitoring (SpaceLabs 90207), carotid ultrasound and pulse wave velocity measurements. Genotypes were determined by allele-specific hybridization.
Genotype frequencies (42.3% for CC, 50.9% for CT and 6.8% for TT) did not deviate from Hardy-Weinberg equilibrium (p = 0.21). Among parents but not among offspring 24-hour, day-time and night-time systolic blood pressures were 5-7 mm Hg higher in TT homozygotes then in C allele carriers (p = 0.04). Similarly, in parents TT homozygotes compared to C-allele carriers had increased pulse wave velocity (11.9 ± 2.2 vs. 10.4 ± 1.5 m/s). No differences in features influenced by gentotype were found in the offspring. In our study we found that the influence of GNB3 C825T polymorphism on blood pressure shows different pattern in the two generations. It is associated with higher systolic blood pressure and higher pulse wave velocity only in parent generation. This observation supports the thesis about recessive model of phenotypic effect of GNB3 polymorphism, which can be detected only in older age-generation group.
We studied 86 nuclear families - 142 parents (age 50.3 ± 5.0 years) and 184 offspring (age 24.0 ± 4.7 years) enrolled in the population-based study in Cracow. Each subject underwent the 24-hr ambulatory blood pressure monitoring (SpaceLabs 90207), carotid ultrasound and pulse wave velocity measurements. Genotypes were determined by allele-specific hybridization.
Genotype frequencies (42.3% for CC, 50.9% for CT and 6.8% for TT) did not deviate from Hardy-Weinberg equilibrium (p = 0.21). Among parents but not among offspring 24-hour, day-time and night-time systolic blood pressures were 5-7 mm Hg higher in TT homozygotes then in C allele carriers (p = 0.04). Similarly, in parents TT homozygotes compared to C-allele carriers had increased pulse wave velocity (11.9 ± 2.2 vs. 10.4 ± 1.5 m/s). No differences in features influenced by gentotype were found in the offspring. In our study we found that the influence of GNB3 C825T polymorphism on blood pressure shows different pattern in the two generations. It is associated with higher systolic blood pressure and higher pulse wave velocity only in parent generation. This observation supports the thesis about recessive model of phenotypic effect of GNB3 polymorphism, which can be detected only in older age-generation group.
Keywords: hypertensiongeneticsGNB3arterial compliance