open access

Vol 10, No 2 (2006)
Prace oryginalne
Published online: 2006-04-18
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Inflammatory state, blood pressure and progression of vascular wall injury in experimental chronic kidney disease

Andrzej Brymora, Mariusz Flisiński, Grzegorz Grześk, Leszek Szadujkis-Szadurski, Jacek Manitius
Nadciśnienie tętnicze 2006;10(2):111-120.

open access

Vol 10, No 2 (2006)
Prace oryginalne
Published online: 2006-04-18

Abstract

Background The inflammation is a well-documented factor influencing the development of cardiovascular complications in chronic kidney disease (CKD) and hypertension. The aim of this study was to examine systemic inflammatory state defined by level of serum haptoglobin, local inflammation defined by monocyte chemotactic protein (MCP-1) level, blood pressure and arterial response to phenylephrine (PHE) and angiotensin II (ANG II) in a different stages of renal failure.
Material and methods Experiments were performed on male Wistar rats, weighing 290-380 g. The rats were divided into four groups: I (control) - shame-operated (n = 12), II - 1/2 nephrectomy (n = 12), III - 3/4 nephrectomy (n = 8), IV - 5/6 nephrectomy (n = 12). After 4 weeks blood pressure (BP) in carotid artery was measured and the blood was collected for BUN, creatinine, albumin, haptoglobin and MCP-1. Then we compared the smooth muscle contractility of rat tail artery after stimulation with PHE and ANG II in all groups. The constriction of artery was measured as an increase in perfusion pressure at a constant flow of the perfusion fluid. Cumulative response curves (CRCs) were obtained using van Rossum method.
Results We observed the increased arterial reaction to agonists in the first stages of renal failure (PHE: group II, III; ANG II: group II) and diminished vasoconstruction reaction in 5/6 nephrectomy. Systemic inflammation defined by haptoglobin level occured in 1/2 nephrectomy group and did not increase in more advanced stages of renal disease. The local inflammation (MCP-1-level) increased in paralel to the renal failure progression.
Conclusions The inflammatory state plays a key role in determining of vascular contraction and resistant artery tone.

Abstract

Background The inflammation is a well-documented factor influencing the development of cardiovascular complications in chronic kidney disease (CKD) and hypertension. The aim of this study was to examine systemic inflammatory state defined by level of serum haptoglobin, local inflammation defined by monocyte chemotactic protein (MCP-1) level, blood pressure and arterial response to phenylephrine (PHE) and angiotensin II (ANG II) in a different stages of renal failure.
Material and methods Experiments were performed on male Wistar rats, weighing 290-380 g. The rats were divided into four groups: I (control) - shame-operated (n = 12), II - 1/2 nephrectomy (n = 12), III - 3/4 nephrectomy (n = 8), IV - 5/6 nephrectomy (n = 12). After 4 weeks blood pressure (BP) in carotid artery was measured and the blood was collected for BUN, creatinine, albumin, haptoglobin and MCP-1. Then we compared the smooth muscle contractility of rat tail artery after stimulation with PHE and ANG II in all groups. The constriction of artery was measured as an increase in perfusion pressure at a constant flow of the perfusion fluid. Cumulative response curves (CRCs) were obtained using van Rossum method.
Results We observed the increased arterial reaction to agonists in the first stages of renal failure (PHE: group II, III; ANG II: group II) and diminished vasoconstruction reaction in 5/6 nephrectomy. Systemic inflammation defined by haptoglobin level occured in 1/2 nephrectomy group and did not increase in more advanced stages of renal disease. The local inflammation (MCP-1-level) increased in paralel to the renal failure progression.
Conclusions The inflammatory state plays a key role in determining of vascular contraction and resistant artery tone.
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Keywords

inflammtory state; arterial reaction; chronic renal disease

About this article
Title

Inflammatory state, blood pressure and progression of vascular wall injury in experimental chronic kidney disease

Journal

Arterial Hypertension

Issue

Vol 10, No 2 (2006)

Pages

111-120

Published online

2006-04-18

Bibliographic record

Nadciśnienie tętnicze 2006;10(2):111-120.

Keywords

inflammtory state
arterial reaction
chronic renal disease

Authors

Andrzej Brymora
Mariusz Flisiński
Grzegorz Grześk
Leszek Szadujkis-Szadurski
Jacek Manitius

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