open access

Vol 5, No 3 (2000)
Original papers
Published online: 2000-11-01
Submitted: 1999-11-23
Get Citation

DNA Ploidy, Bromodeoxyuridine labelling index, S-phase fraction and AgNOR counts in brain tumours

Gasińska Anna, Niemiec Joanna, Adamczyk Agnieszka
DOI: 10.1016/S1507-1367(00)70364-6
·
Rep Pract Oncol Radiother 2000;5(3):75-81.

open access

Vol 5, No 3 (2000)
Original papers
Published online: 2000-11-01
Submitted: 1999-11-23

Abstract

DNA ploidy and the proliferative potential in 88 brain tumours were investigated using the bromodeoxyuridine labelling index (BrdUrd LI), S-phase fraction (SPF) and an argyrophilic nucleolar organizer regions (AgNOR) technique. The study included 65 highly malignant (AIII–AIV), and 23 low-grade (AI–AII) gliomas. One fragment of the tumour was fixed in Carnoy's solution for AgNOR test, while the other fragments were used for flow cytometric determination of the labelling index, SPF and DNA ploidy. For the BrdUrdLl, tumour samples from each patient were incubated in vitro for one hour at 37°C with BrdUrd using a high pressure oxygen method. After fixation and staining, the percentages of BrdUrd-labelied cells (BrdUrdLI) and unlabelled S-phase cells (SPF) were evaluated. The tumours showed variability in the BrdUrdLI values, SPF and AgNOR counts/cell nucleus. However, grade dependent differences in the proliferating rate were only found to exist on the basis of BrdUrdLI and AgNOR counts. The same percentage of DNA aneuploidy (56 %) was found in high-grade as well as in low-grade gliomas. A linear – regression analysis showed a significant correlation between the results of three applied methods: BrdUrdLl, SPF and AgNOR counts.

Abstract

DNA ploidy and the proliferative potential in 88 brain tumours were investigated using the bromodeoxyuridine labelling index (BrdUrd LI), S-phase fraction (SPF) and an argyrophilic nucleolar organizer regions (AgNOR) technique. The study included 65 highly malignant (AIII–AIV), and 23 low-grade (AI–AII) gliomas. One fragment of the tumour was fixed in Carnoy's solution for AgNOR test, while the other fragments were used for flow cytometric determination of the labelling index, SPF and DNA ploidy. For the BrdUrdLl, tumour samples from each patient were incubated in vitro for one hour at 37°C with BrdUrd using a high pressure oxygen method. After fixation and staining, the percentages of BrdUrd-labelied cells (BrdUrdLI) and unlabelled S-phase cells (SPF) were evaluated. The tumours showed variability in the BrdUrdLI values, SPF and AgNOR counts/cell nucleus. However, grade dependent differences in the proliferating rate were only found to exist on the basis of BrdUrdLI and AgNOR counts. The same percentage of DNA aneuploidy (56 %) was found in high-grade as well as in low-grade gliomas. A linear – regression analysis showed a significant correlation between the results of three applied methods: BrdUrdLl, SPF and AgNOR counts.

Get Citation

Keywords

Bromodeoxyuridine labelling index; SPF; Nucleolar organizer regions (AgNOR); Proliferation rate; DNA ploidy; Brain tumours

About this article
Title

DNA Ploidy, Bromodeoxyuridine labelling index, S-phase fraction and AgNOR counts in brain tumours

Journal

Reports of Practical Oncology and Radiotherapy

Issue

Vol 5, No 3 (2000)

Pages

75-81

Published online

2000-11-01

DOI

10.1016/S1507-1367(00)70364-6

Bibliographic record

Rep Pract Oncol Radiother 2000;5(3):75-81.

Keywords

Bromodeoxyuridine labelling index
SPF
Nucleolar organizer regions (AgNOR)
Proliferation rate
DNA ploidy
Brain tumours

Authors

Gasińska Anna
Niemiec Joanna
Adamczyk Agnieszka

Important: This website uses cookies. More >>

The cookies allow us to identify your computer and find out details about your last visit. They remembering whether you've visited the site before, so that you remain logged in - or to help us work out how many new website visitors we get each month. Most internet browsers accept cookies automatically, but you can change the settings of your browser to erase cookies or prevent automatic acceptance if you prefer.

By "Via Medica sp. z o.o." sp.k., ul. Świętokrzyska 73, 80–180 Gdańsk, Poland
tel.:+48 58 320 94 94, fax:+48 58 320 94 60, e-mail: journals@viamedica.pl