open access

Vol 25, No 5 (2020)
Original research articles
Published online: 2020-09-01
Submitted: 2020-01-09
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Apoptosis related microRNAs and MGMT in glioblastoma cell lines submitted to treatments with ionizing radiation and temozolomide

Felipe Amstalden Trevisan, Andressa Romualdo Rodrigues, Fermino Sanches Lizarte Neto, Fernanda Maris Peria, Múcio Luiz Cirino, Daniela Pretti Tirapelli, Carlos Gilberto Carlotti Júnior
DOI: 10.1016/j.rpor.2020.06.007
·
Rep Pract Oncol Radiother 2020;25(5):714-719.

open access

Vol 25, No 5 (2020)
Original research articles
Published online: 2020-09-01
Submitted: 2020-01-09

Abstract

Aim

To evaluate the effect of radiotherapy and temozolomide on the expression of miRNAs apoptotic (miRNAs-21, -221, -222 (anti-apoptotic) and miRNAs-15a, -16 (pro-apoptotic)) and the gene MGMT in glioblastoma cell lines.

Background

The limited knowledge of the molecular biology of malignant gliomas may hinder the development of therapeutic modalities. In this scenario, one of the greatest advances of recent years was the identification of microRNAs. These molecules have an important role in biological processes involving cancer, including glioblastoma.

Materials and methods

Trypan blue was used to verify the cell viability, and real time PCR to quantify the expression of microRNAs and gene 24, 48 and 120 h after exposure to treatments.

Results

There was a statistically significant decrease of expression of miR-15a between 48 and 120 h in line T98 G treated with radiation, increased expression of miR-15a between 24 and 120 h in line U251 treated with radiation and temozolomide, and increased expression of miR-16 between 24 and 120 h in line U251 treated with radiation alone and when combined with temozolomide. There was a decrease in MGMT gene expression, between 24 and 48 h in U343 cells treated with temozolomide.

Conclusions

Ionizing radiation and temozolomide modified the expression of miRNAs studied and MGMT.

Abstract

Aim

To evaluate the effect of radiotherapy and temozolomide on the expression of miRNAs apoptotic (miRNAs-21, -221, -222 (anti-apoptotic) and miRNAs-15a, -16 (pro-apoptotic)) and the gene MGMT in glioblastoma cell lines.

Background

The limited knowledge of the molecular biology of malignant gliomas may hinder the development of therapeutic modalities. In this scenario, one of the greatest advances of recent years was the identification of microRNAs. These molecules have an important role in biological processes involving cancer, including glioblastoma.

Materials and methods

Trypan blue was used to verify the cell viability, and real time PCR to quantify the expression of microRNAs and gene 24, 48 and 120 h after exposure to treatments.

Results

There was a statistically significant decrease of expression of miR-15a between 48 and 120 h in line T98 G treated with radiation, increased expression of miR-15a between 24 and 120 h in line U251 treated with radiation and temozolomide, and increased expression of miR-16 between 24 and 120 h in line U251 treated with radiation alone and when combined with temozolomide. There was a decrease in MGMT gene expression, between 24 and 48 h in U343 cells treated with temozolomide.

Conclusions

Ionizing radiation and temozolomide modified the expression of miRNAs studied and MGMT.

Get Citation

Keywords

Glioblastoma; microRNAs; Ionizing radiation; Apoptosis

About this article
Title

Apoptosis related microRNAs and MGMT in glioblastoma cell lines submitted to treatments with ionizing radiation and temozolomide

Journal

Reports of Practical Oncology and Radiotherapy

Issue

Vol 25, No 5 (2020)

Pages

714-719

Published online

2020-09-01

DOI

10.1016/j.rpor.2020.06.007

Bibliographic record

Rep Pract Oncol Radiother 2020;25(5):714-719.

Keywords

Glioblastoma
microRNAs
Ionizing radiation
Apoptosis

Authors

Felipe Amstalden Trevisan
Andressa Romualdo Rodrigues
Fermino Sanches Lizarte Neto
Fernanda Maris Peria
Múcio Luiz Cirino
Daniela Pretti Tirapelli
Carlos Gilberto Carlotti Júnior

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