open access

Vol 24, No 2 (2019)
Reviews
Published online: 2019-03-01
Submitted: 2018-06-15
Get Citation

Gene delivery methods and genome editing of human pluripotent stem cells

Patrycja Czerwińska, Sylwia Mazurek, Iga Kołodziejczak, Maciej Wiznerowicz
DOI: 10.1016/j.rpor.2019.01.007
·
Rep Pract Oncol Radiother 2019;24(2):180-187.

open access

Vol 24, No 2 (2019)
Reviews
Published online: 2019-03-01
Submitted: 2018-06-15

Abstract

Induced pluripotent stem cells derived from normal somatic cells could be utilized to study tumorigenesis through overexpression of specific oncogenes, downregulation of tumor suppressors and dysregulation of other factors thought to promote tumorigenesis. Therefore, effective approaches that provide direct modifications of induced pluripotent stem cell genome are extremely needed. Emerging strategies are expected to provide the ability to more effectively introduce diverse genetic alterations, from as small as single-nucleotide modifications to whole gene amplification or deletion, all with a high degree of target specificity. To date, several techniques have been applied in stem cell studies to directly edit cell genome (ZFNs, TALENs or CRISPR/Cas9). In this review, we summarize specific gene delivery strategies that were applied to stem cell studies together with genome editing techniques, which enable a direct modification of endogenous DNA sequences in the context of cancer studies.

Abstract

Induced pluripotent stem cells derived from normal somatic cells could be utilized to study tumorigenesis through overexpression of specific oncogenes, downregulation of tumor suppressors and dysregulation of other factors thought to promote tumorigenesis. Therefore, effective approaches that provide direct modifications of induced pluripotent stem cell genome are extremely needed. Emerging strategies are expected to provide the ability to more effectively introduce diverse genetic alterations, from as small as single-nucleotide modifications to whole gene amplification or deletion, all with a high degree of target specificity. To date, several techniques have been applied in stem cell studies to directly edit cell genome (ZFNs, TALENs or CRISPR/Cas9). In this review, we summarize specific gene delivery strategies that were applied to stem cell studies together with genome editing techniques, which enable a direct modification of endogenous DNA sequences in the context of cancer studies.

Get Citation

Keywords

Genome editing; Stem cells; Induced pluripotent stem cells; ZFN; TALEN; CRISPR/Cas9

About this article
Title

Gene delivery methods and genome editing of human pluripotent stem cells

Journal

Reports of Practical Oncology and Radiotherapy

Issue

Vol 24, No 2 (2019)

Pages

180-187

Published online

2019-03-01

DOI

10.1016/j.rpor.2019.01.007

Bibliographic record

Rep Pract Oncol Radiother 2019;24(2):180-187.

Keywords

Genome editing
Stem cells
Induced pluripotent stem cells
ZFN
TALEN
CRISPR/Cas9

Authors

Patrycja Czerwińska
Sylwia Mazurek
Iga Kołodziejczak
Maciej Wiznerowicz

Important: This website uses cookies. More >>

The cookies allow us to identify your computer and find out details about your last visit. They remembering whether you've visited the site before, so that you remain logged in - or to help us work out how many new website visitors we get each month. Most internet browsers accept cookies automatically, but you can change the settings of your browser to erase cookies or prevent automatic acceptance if you prefer.

By "Via Medica sp. z o.o." sp.k., ul. Świętokrzyska 73, 80–180 Gdańsk, Poland
tel.:+48 58 320 94 94, fax:+48 58 320 94 60, e-mail: journals@viamedica.pl