open access

Vol 22, No 4 (2017)
Original research articles
Published online: 2017-07-01
Submitted: 2016-06-29
Get Citation

Biodegradable seeds of holmium don’t change neurological function after implant in brain of rats

Mirla Fiuza Diniz, Diogo Milioli Ferreira, Wanderson Geraldo de Lima, Maria Lucia Pedrosa, Marcelo Eustáquio Silva, Stanley de Almeida Araujo, Kinulpe Honorato Sampaio, Tarcisio Passos de Campos, Savio Lana Siqueira
DOI: 10.1016/j.rpor.2017.03.003
·
Rep Pract Oncol Radiother 2017;22(4):319-326.

open access

Vol 22, No 4 (2017)
Original research articles
Published online: 2017-07-01
Submitted: 2016-06-29

Abstract

Aim

To evaluate the surgical procedure and parenchymal abnormalities related to implantation of ceramic seeds with holmium-165 in rats’ brain.

Background

An effective method of cancer treatment is brachytherapy in which radioactive seeds are implanted in the tumor, generating a high local dose of ionizing radiation that can eliminate tumor cells while protecting the surrounding healthy tissue. Biodegradable Ho166-ceramic-seeds have been addressed recently.

Methods and materials

The experiments in this study were approved by the Ethics Committee on Animal Use at the Federal University of Ouro Preto, protocol number 2012/034. Twenty-one adult Fischer rats were divided into Naive Group, Sham Group and Group for seed implants (ISH). Surgical procedures for implantation of biodegradable seeds were done and 30 days after the implant radiographic examination and biopsy of the brain were performed. Neurological assays were also accomplished to exclude any injury resulting from either surgery or implantation of the seeds.

Results

Radiographic examination confirmed the location of the seeds in the brain. Neurological assays showed animals with regular spontaneous activity. The histological analysis showed an increase of inflammatory cells in the brain of the ISH group. Electron microscopy evidenced cytoplasmic organelles to be unchanged. Biochemical analyzes indicate there was neither oxidative stress nor oxidative damage in the ISH brain. CAT activity showed no difference between the groups as well as lipid peroxidation measured by TBARS.

Conclusions

The analysis of the data pointed out that the performed procedure is safe as no animal showed alterations of the neurological parameters and the seeds did not promote histological architectural changes in the brain tissue.

Abstract

Aim

To evaluate the surgical procedure and parenchymal abnormalities related to implantation of ceramic seeds with holmium-165 in rats’ brain.

Background

An effective method of cancer treatment is brachytherapy in which radioactive seeds are implanted in the tumor, generating a high local dose of ionizing radiation that can eliminate tumor cells while protecting the surrounding healthy tissue. Biodegradable Ho166-ceramic-seeds have been addressed recently.

Methods and materials

The experiments in this study were approved by the Ethics Committee on Animal Use at the Federal University of Ouro Preto, protocol number 2012/034. Twenty-one adult Fischer rats were divided into Naive Group, Sham Group and Group for seed implants (ISH). Surgical procedures for implantation of biodegradable seeds were done and 30 days after the implant radiographic examination and biopsy of the brain were performed. Neurological assays were also accomplished to exclude any injury resulting from either surgery or implantation of the seeds.

Results

Radiographic examination confirmed the location of the seeds in the brain. Neurological assays showed animals with regular spontaneous activity. The histological analysis showed an increase of inflammatory cells in the brain of the ISH group. Electron microscopy evidenced cytoplasmic organelles to be unchanged. Biochemical analyzes indicate there was neither oxidative stress nor oxidative damage in the ISH brain. CAT activity showed no difference between the groups as well as lipid peroxidation measured by TBARS.

Conclusions

The analysis of the data pointed out that the performed procedure is safe as no animal showed alterations of the neurological parameters and the seeds did not promote histological architectural changes in the brain tissue.

Get Citation

Keywords

Brachytherapy; Tumor; Seeds; Holmium; Brain; Morphometry

About this article
Title

Biodegradable seeds of holmium don’t change neurological function after implant in brain of rats

Journal

Reports of Practical Oncology and Radiotherapy

Issue

Vol 22, No 4 (2017)

Pages

319-326

Published online

2017-07-01

DOI

10.1016/j.rpor.2017.03.003

Bibliographic record

Rep Pract Oncol Radiother 2017;22(4):319-326.

Keywords

Brachytherapy
Tumor
Seeds
Holmium
Brain
Morphometry

Authors

Mirla Fiuza Diniz
Diogo Milioli Ferreira
Wanderson Geraldo de Lima
Maria Lucia Pedrosa
Marcelo Eustáquio Silva
Stanley de Almeida Araujo
Kinulpe Honorato Sampaio
Tarcisio Passos de Campos
Savio Lana Siqueira

Important: This website uses cookies. More >>

The cookies allow us to identify your computer and find out details about your last visit. They remembering whether you've visited the site before, so that you remain logged in - or to help us work out how many new website visitors we get each month. Most internet browsers accept cookies automatically, but you can change the settings of your browser to erase cookies or prevent automatic acceptance if you prefer.

By "Via Medica sp. z o.o." sp.k., ul. Świętokrzyska 73, 80–180 Gdańsk, Poland
tel.:+48 58 320 94 94, fax:+48 58 320 94 60, e-mail: journals@viamedica.pl