open access

Vol 21, No 2 (2016)
Special Issue Papers
Published online: 2016-03-01
Submitted: 2014-04-22
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Experimental set up for the irradiation of biological samples and nuclear track detectors with UV C

Agustina Mariana Portu, Andrés Eugenio Rossini, Mario Alberto Gadan, Omar Alberto Bernaola, Silvia Inés Thorp, Paula Curotto, Emiliano César Pozzi, Rómulo Luis Cabrini, Gisela Saint Martin
DOI: 10.1016/j.rpor.2014.10.003
·
Rep Pract Oncol Radiother 2016;21(2):129-134.

open access

Vol 21, No 2 (2016)
Special Issue Papers
Published online: 2016-03-01
Submitted: 2014-04-22

Abstract

Aim

In this work we present a methodology to produce an “imprint” of cells cultivated on a polycarbonate detector by exposure of the detector to UV C radiation.

Background

The distribution and concentration of 10B atoms in tissue samples coming from BNCT (Boron Neutron Capture Therapy) protocols can be determined through the quantification and analysis of the tracks forming its autoradiography image on a nuclear track detector. The location of boron atoms in the cell structure could be known more accurately by the simultaneous observation of the nuclear tracks and the sample image on the detector.

Materials and Methods

A UV C irradiator was constructed. The irradiance was measured along the lamp direction and at different distances. Melanoma cells were cultured on polycarbonate foils, incubated with borophenylalanine, irradiated with thermal neutrons and exposed to UV C radiation. The samples were chemically attacked with a KOH solution.

Results

A uniform irradiation field was established to expose the detector foils to UV C light. Cells could be seeded on the polycarbonate surface. Both imprints from cells and nuclear tracks were obtained after chemical etching.

Conclusions

It is possible to yield cellular imprints in polycarbonate. The nuclear tracks were mostly present inside the cells, indicating a preferential boron uptake.

Abstract

Aim

In this work we present a methodology to produce an “imprint” of cells cultivated on a polycarbonate detector by exposure of the detector to UV C radiation.

Background

The distribution and concentration of 10B atoms in tissue samples coming from BNCT (Boron Neutron Capture Therapy) protocols can be determined through the quantification and analysis of the tracks forming its autoradiography image on a nuclear track detector. The location of boron atoms in the cell structure could be known more accurately by the simultaneous observation of the nuclear tracks and the sample image on the detector.

Materials and Methods

A UV C irradiator was constructed. The irradiance was measured along the lamp direction and at different distances. Melanoma cells were cultured on polycarbonate foils, incubated with borophenylalanine, irradiated with thermal neutrons and exposed to UV C radiation. The samples were chemically attacked with a KOH solution.

Results

A uniform irradiation field was established to expose the detector foils to UV C light. Cells could be seeded on the polycarbonate surface. Both imprints from cells and nuclear tracks were obtained after chemical etching.

Conclusions

It is possible to yield cellular imprints in polycarbonate. The nuclear tracks were mostly present inside the cells, indicating a preferential boron uptake.

Get Citation

Keywords

BNCT; UV-C; Polycarbonate detectors; Cell imprints

About this article
Title

Experimental set up for the irradiation of biological samples and nuclear track detectors with UV C

Journal

Reports of Practical Oncology and Radiotherapy

Issue

Vol 21, No 2 (2016)

Pages

129-134

Published online

2016-03-01

DOI

10.1016/j.rpor.2014.10.003

Bibliographic record

Rep Pract Oncol Radiother 2016;21(2):129-134.

Keywords

BNCT
UV-C
Polycarbonate detectors
Cell imprints

Authors

Agustina Mariana Portu
Andrés Eugenio Rossini
Mario Alberto Gadan
Omar Alberto Bernaola
Silvia Inés Thorp
Paula Curotto
Emiliano César Pozzi
Rómulo Luis Cabrini
Gisela Saint Martin

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