open access

Vol 19, No 1 (2014)
Supplement Papers
Published online: 2014-01-01
Submitted: 2013-08-29
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Investigation of the bystander effect in CHO-K1 cells

Urszula Kaźmierczak, Dariusz Banaś, Janusz Braziewicz, Iwona Buraczewska, Joanna Czub, Marian Jaskóła, Łukasz Kaźmierczak, Andrzej Korman, Marcin Kruszewski, Anna Lankoff, Halina Lisowska, Marta Nesteruk, Zygmunt Szefliński, Maria Wojewódzka
DOI: 10.1016/j.rpor.2014.04.015
·
Rep Pract Oncol Radiother 2014;19(1).

open access

Vol 19, No 1 (2014)
Supplement Papers
Published online: 2014-01-01
Submitted: 2013-08-29

Abstract

Aim

Investigation of the bystander effect in Chinese Hamster Ovary cells (CHO-K1) co-cultured with cells irradiated in the dose range of 0.1–4[[ce:hsp sp="0.25"/]]Gy of high LET 12C ions and X-rays.

Background

The radiobiological effects of charged heavy particles on a cellular or molecular level are of fundamental importance in the field of biomedical applications, especially in hadron therapy and space radiation biology.

Materials and methods

A heavy ion 12C beam from the Heavy Ion Laboratory of the University of Warsaw (HIL) was used to irradiate CHO-K1 cells. Cells were seeded in Petri dishes specially designed for irradiation purposes. Immediately after irradiation, cells were transferred into transwell culture insert dishes to enable co-culture of irradiated and non-irradiated cells. Cells from the membrane and well shared the medium but could not touch each other. To study bystander effects, a clonogenic survival assay was performed.

Results

The survival fraction of cells co-cultured with cells irradiated with 12C ions and X-rays was not reduced.

Conclusions

The bystander effect was not observed in these studies.

Abstract

Aim

Investigation of the bystander effect in Chinese Hamster Ovary cells (CHO-K1) co-cultured with cells irradiated in the dose range of 0.1–4[[ce:hsp sp="0.25"/]]Gy of high LET 12C ions and X-rays.

Background

The radiobiological effects of charged heavy particles on a cellular or molecular level are of fundamental importance in the field of biomedical applications, especially in hadron therapy and space radiation biology.

Materials and methods

A heavy ion 12C beam from the Heavy Ion Laboratory of the University of Warsaw (HIL) was used to irradiate CHO-K1 cells. Cells were seeded in Petri dishes specially designed for irradiation purposes. Immediately after irradiation, cells were transferred into transwell culture insert dishes to enable co-culture of irradiated and non-irradiated cells. Cells from the membrane and well shared the medium but could not touch each other. To study bystander effects, a clonogenic survival assay was performed.

Results

The survival fraction of cells co-cultured with cells irradiated with 12C ions and X-rays was not reduced.

Conclusions

The bystander effect was not observed in these studies.

Get Citation

Keywords

[[ce:sup loc=" pre" ]]12C-beam; Bystander effect; CHO-K1 cells; Clonogenic survival assay

About this article
Title

Investigation of the bystander effect in CHO-K1 cells

Journal

Reports of Practical Oncology and Radiotherapy

Issue

Vol 19, No 1 (2014)

Published online

2014-01-01

DOI

10.1016/j.rpor.2014.04.015

Bibliographic record

Rep Pract Oncol Radiother 2014;19(1).

Keywords

[[ce:sup loc="pre"]]12C-beam
Bystander effect
CHO-K1 cells
Clonogenic survival assay

Authors

Urszula Kaźmierczak
Dariusz Banaś
Janusz Braziewicz
Iwona Buraczewska
Joanna Czub
Marian Jaskóła
Łukasz Kaźmierczak
Andrzej Korman
Marcin Kruszewski
Anna Lankoff
Halina Lisowska
Marta Nesteruk
Zygmunt Szefliński
Maria Wojewódzka

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