open access

Vol 16, No 4 (2011)
Special Issue Papers
Published online: 2011-07-01
Submitted: 2011-03-29
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Biodosimetry and assessment of radiation dose

Rafael Herranz Crespo, Mercedes Moreno Domene, María Jesús Rodríguez
DOI: 10.1016/j.rpor.2011.06.003
·
Rep Pract Oncol Radiother 2011;16(4):131-137.

open access

Vol 16, No 4 (2011)
Special Issue Papers
Published online: 2011-07-01
Submitted: 2011-03-29

Abstract

Aim

When investigating radiation accidents, it is very important to determine the exposition dose to the individuals. In the case of exposures over 1[[ce:hsp sp="0.25"/]]Gy, clinicians may expect deterministic effects arising the following weeks and months, in these cases dose estimation will help physicians in the planning of therapy. Nevertheless, for doses below 1[[ce:hsp sp="0.25"/]]Gy, biodosimetry data are important due to the risk of developing late stochastic effects. Finally, some accidental overexposures are lack of physical measurements and the only way of quantifying dose is by biological dosimetry.

Background

The analysis of chromosomal aberrations by different techniques is the most developed method of quantifying dose to individuals exposed to ionising radiations.[[ce:cross-refs refid="bib0005 bib0010"]]1,2 Furthermore, the analysis of dicentric chromosomes observed in metaphases from peripheral lymphocytes is the routine technique used in case of acute exposures to assess radiation doses.

Materials and methods

Solid stain of chromosomes is used to determine dicentric yields for dose estimation. Fluorescence in situ hybridization (FISH) for translocations analysis is used when delayed sampling or suspected chronically irradiation dose assessment. Recommendations in technical considerations are based mainly in the IAEA Technical Report No. 405.[[ce:cross-ref refid="bib0010"]]2

Results

Experience in biological dosimetry at Gregorio Marañón General Hospital is described, including own calibration curves used for dose estimation, background studies and real cases of overexposition.

Conclusion

Dose assessment by biological dosimeters requires a large previous standardization work and a continuous update. Individual dose assessment involves high qualification professionals and its long time consuming, therefore requires specific Centres. For large mass casualties cooperation among specialized Institutions is needed.

Abstract

Aim

When investigating radiation accidents, it is very important to determine the exposition dose to the individuals. In the case of exposures over 1[[ce:hsp sp="0.25"/]]Gy, clinicians may expect deterministic effects arising the following weeks and months, in these cases dose estimation will help physicians in the planning of therapy. Nevertheless, for doses below 1[[ce:hsp sp="0.25"/]]Gy, biodosimetry data are important due to the risk of developing late stochastic effects. Finally, some accidental overexposures are lack of physical measurements and the only way of quantifying dose is by biological dosimetry.

Background

The analysis of chromosomal aberrations by different techniques is the most developed method of quantifying dose to individuals exposed to ionising radiations.[[ce:cross-refs refid="bib0005 bib0010"]]1,2 Furthermore, the analysis of dicentric chromosomes observed in metaphases from peripheral lymphocytes is the routine technique used in case of acute exposures to assess radiation doses.

Materials and methods

Solid stain of chromosomes is used to determine dicentric yields for dose estimation. Fluorescence in situ hybridization (FISH) for translocations analysis is used when delayed sampling or suspected chronically irradiation dose assessment. Recommendations in technical considerations are based mainly in the IAEA Technical Report No. 405.[[ce:cross-ref refid="bib0010"]]2

Results

Experience in biological dosimetry at Gregorio Marañón General Hospital is described, including own calibration curves used for dose estimation, background studies and real cases of overexposition.

Conclusion

Dose assessment by biological dosimeters requires a large previous standardization work and a continuous update. Individual dose assessment involves high qualification professionals and its long time consuming, therefore requires specific Centres. For large mass casualties cooperation among specialized Institutions is needed.

Get Citation

Keywords

Biodosimetry; Dicentrics; Translocations; FISH

About this article
Title

Biodosimetry and assessment of radiation dose

Journal

Reports of Practical Oncology and Radiotherapy

Issue

Vol 16, No 4 (2011)

Pages

131-137

Published online

2011-07-01

DOI

10.1016/j.rpor.2011.06.003

Bibliographic record

Rep Pract Oncol Radiother 2011;16(4):131-137.

Keywords

Biodosimetry
Dicentrics
Translocations
FISH

Authors

Rafael Herranz Crespo
Mercedes Moreno Domene
María Jesús Rodríguez

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