open access

Vol 14, No 3 (2009)
Untitled
Published online: 2009-05-01
Submitted: 2009-05-20
Get Citation

Influence of 6 MV and 20 MV X-radiation dose rate on in vitro survive of the K-562 cell line

Mariusz GRUDA, Grażyna KOSICKA, Ewelina KONSTANTY, Dariusz KOWALCZYK
DOI: 10.1016/S1507-1367(10)60099-5
·
Rep Pract Oncol Radiother 2009;14(3):85-88.

open access

Vol 14, No 3 (2009)
Untitled
Published online: 2009-05-01
Submitted: 2009-05-20

Abstract

Background

Analysis of the survival rate of cells after irradiation with a specified dose of X-radiation might be one of the basic foundations for assessment of biological implications of ionizing radiation. Investigation of the influence of X-radiation dose rate on cells was carried out in vitro using the SF2 test.

Aim

The aim of this study was to investigate the influence of X-radiation dose rate on the surviving fraction of the K-562 cell line for two photon energies of 6 MV and 20 MV.

Materials/Methods

To measure the cells' reaction to X-radiation of variable dose rate human leukaemic K-562 cells were used. In order to fulfil the main aim of the study, the cell line was subjected to irradiation at two different dose rates. Total dose applied at once was 2 Gy. A quantitative evaluation of cell survival rate was carried out at every step of the experiment using a clonogenic assay.

Results

High dose rate at the energy of 6 MV decreased the percentage of surviving cells to 23%, while lower dose rate decreased it only to 36%. A similar effect is observed at the energy of 20MV-namely at the higher dose rate the percentage of surviving cells is 18%, whereas at the lower one it is only 34%.

Conclusions

The experiment has shown that when using a lower dose rate, the biological effect of ionizing radiation is less pronounced. However, at a higher dose rate higher radiosensitivity of cells is observed.

Abstract

Background

Analysis of the survival rate of cells after irradiation with a specified dose of X-radiation might be one of the basic foundations for assessment of biological implications of ionizing radiation. Investigation of the influence of X-radiation dose rate on cells was carried out in vitro using the SF2 test.

Aim

The aim of this study was to investigate the influence of X-radiation dose rate on the surviving fraction of the K-562 cell line for two photon energies of 6 MV and 20 MV.

Materials/Methods

To measure the cells' reaction to X-radiation of variable dose rate human leukaemic K-562 cells were used. In order to fulfil the main aim of the study, the cell line was subjected to irradiation at two different dose rates. Total dose applied at once was 2 Gy. A quantitative evaluation of cell survival rate was carried out at every step of the experiment using a clonogenic assay.

Results

High dose rate at the energy of 6 MV decreased the percentage of surviving cells to 23%, while lower dose rate decreased it only to 36%. A similar effect is observed at the energy of 20MV-namely at the higher dose rate the percentage of surviving cells is 18%, whereas at the lower one it is only 34%.

Conclusions

The experiment has shown that when using a lower dose rate, the biological effect of ionizing radiation is less pronounced. However, at a higher dose rate higher radiosensitivity of cells is observed.

Get Citation

Keywords

K-562 cells; SF2 test; radiosensitivity of cells; clonogenic assay

About this article
Title

Influence of 6 MV and 20 MV X-radiation dose rate on in vitro survive of the K-562 cell line

Journal

Reports of Practical Oncology and Radiotherapy

Issue

Vol 14, No 3 (2009)

Pages

85-88

Published online

2009-05-01

DOI

10.1016/S1507-1367(10)60099-5

Bibliographic record

Rep Pract Oncol Radiother 2009;14(3):85-88.

Keywords

K-562 cells
SF2 test
radiosensitivity of cells
clonogenic assay

Authors

Mariusz GRUDA
Grażyna KOSICKA
Ewelina KONSTANTY
Dariusz KOWALCZYK

Important: This website uses cookies. More >>

The cookies allow us to identify your computer and find out details about your last visit. They remembering whether you've visited the site before, so that you remain logged in - or to help us work out how many new website visitors we get each month. Most internet browsers accept cookies automatically, but you can change the settings of your browser to erase cookies or prevent automatic acceptance if you prefer.

By "Via Medica sp. z o.o." sp.k., ul. Świętokrzyska 73, 80–180 Gdańsk, Poland
tel.:+48 58 320 94 94, fax:+48 58 320 94 60, e-mail: journals@viamedica.pl