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Long-term changes of prostacyclin secretion in radiation-induced myelopathy
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Abstract
Background
We have previously reported the short-term changes in prostacyclin profile after irradiation of rat cervical cord.
Aim
Present research investigated the long-term changes of prostacyclin content.
Materials/Methods
Wistar rats in groups of five were irradiated with doses of 2, 4, 6, 15, 25, 30 Gy and a single group of 25 with 35 Gy X-rays. After 26 and 39 weeks, prostacyclin content was quantified by 6-keto-prostaglandin-F1α (prostacyclin stabilized metabolite). Specimens were stained routinely for histological studies.
Results
The 50% latent period and effective dose were 14.86±1.16 weeks and 25.66±0.54 Gy (p<0.0001), respectively. Average ratios of 6-keto-PG-F1α for doses of 2–30 Gy were between 78.33–12.93% and 79.48–99.96% for 26 and 39 weeks, respectively. Prostacyclin level after 35 Gy shows approximately a 7:1 ratio in comparison to the control group (p<0.002). Histopathological changes in glial and vascular tissues were diagnosed and scored. Prostacyclin bimodal profile was observed.
Conclusions
Radiation can cause complex fluctuations of prostacyclin in association with marked histopathological changes.
Abstract
Background
We have previously reported the short-term changes in prostacyclin profile after irradiation of rat cervical cord.
Aim
Present research investigated the long-term changes of prostacyclin content.
Materials/Methods
Wistar rats in groups of five were irradiated with doses of 2, 4, 6, 15, 25, 30 Gy and a single group of 25 with 35 Gy X-rays. After 26 and 39 weeks, prostacyclin content was quantified by 6-keto-prostaglandin-F1α (prostacyclin stabilized metabolite). Specimens were stained routinely for histological studies.
Results
The 50% latent period and effective dose were 14.86±1.16 weeks and 25.66±0.54 Gy (p<0.0001), respectively. Average ratios of 6-keto-PG-F1α for doses of 2–30 Gy were between 78.33–12.93% and 79.48–99.96% for 26 and 39 weeks, respectively. Prostacyclin level after 35 Gy shows approximately a 7:1 ratio in comparison to the control group (p<0.002). Histopathological changes in glial and vascular tissues were diagnosed and scored. Prostacyclin bimodal profile was observed.
Conclusions
Radiation can cause complex fluctuations of prostacyclin in association with marked histopathological changes.
Keywords
spinal cord; radiation myelopathy; prostacyclin; histopathology; rat
About this articleTitle
Long-term changes of prostacyclin secretion in radiation-induced myelopathy
Journal
Reports of Practical Oncology and Radiotherapy
Issue
Pages
273-279
Published online
2006-01-01
DOI
10.1016/S1507-1367(06)71072-0
Bibliographic record
Rep Pract Oncol Radiother 2006;11(6):273-279.
Keywords
spinal cord
radiation myelopathy
prostacyclin
histopathology
rat
Authors
Seied Rabie Mahdavi
Alireza Shirazi
Bagher Minaee
Alireza Nikoofar
Hamid Reza Mirzaee