open access

Vol 11, No 6 (2006)
Original papers
Published online: 2006-01-01
Submitted: 2006-10-01
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Long-term changes of prostacyclin secretion in radiation-induced myelopathy

Seied Rabie Mahdavi, Alireza Shirazi, Bagher Minaee, Alireza Nikoofar, Hamid Reza Mirzaee
DOI: 10.1016/S1507-1367(06)71072-0
·
Rep Pract Oncol Radiother 2006;11(6):273-279.

open access

Vol 11, No 6 (2006)
Original papers
Published online: 2006-01-01
Submitted: 2006-10-01

Abstract

Background

We have previously reported the short-term changes in prostacyclin profile after irradiation of rat cervical cord.

Aim

Present research investigated the long-term changes of prostacyclin content.

Materials/Methods

Wistar rats in groups of five were irradiated with doses of 2, 4, 6, 15, 25, 30 Gy and a single group of 25 with 35 Gy X-rays. After 26 and 39 weeks, prostacyclin content was quantified by 6-keto-prostaglandin-F1α (prostacyclin stabilized metabolite). Specimens were stained routinely for histological studies.

Results

The 50% latent period and effective dose were 14.86±1.16 weeks and 25.66±0.54 Gy (p<0.0001), respectively. Average ratios of 6-keto-PG-F1α for doses of 2–30 Gy were between 78.33–12.93% and 79.48–99.96% for 26 and 39 weeks, respectively. Prostacyclin level after 35 Gy shows approximately a 7:1 ratio in comparison to the control group (p<0.002). Histopathological changes in glial and vascular tissues were diagnosed and scored. Prostacyclin bimodal profile was observed.

Conclusions

Radiation can cause complex fluctuations of prostacyclin in association with marked histopathological changes.

Abstract

Background

We have previously reported the short-term changes in prostacyclin profile after irradiation of rat cervical cord.

Aim

Present research investigated the long-term changes of prostacyclin content.

Materials/Methods

Wistar rats in groups of five were irradiated with doses of 2, 4, 6, 15, 25, 30 Gy and a single group of 25 with 35 Gy X-rays. After 26 and 39 weeks, prostacyclin content was quantified by 6-keto-prostaglandin-F1α (prostacyclin stabilized metabolite). Specimens were stained routinely for histological studies.

Results

The 50% latent period and effective dose were 14.86±1.16 weeks and 25.66±0.54 Gy (p<0.0001), respectively. Average ratios of 6-keto-PG-F1α for doses of 2–30 Gy were between 78.33–12.93% and 79.48–99.96% for 26 and 39 weeks, respectively. Prostacyclin level after 35 Gy shows approximately a 7:1 ratio in comparison to the control group (p<0.002). Histopathological changes in glial and vascular tissues were diagnosed and scored. Prostacyclin bimodal profile was observed.

Conclusions

Radiation can cause complex fluctuations of prostacyclin in association with marked histopathological changes.

Get Citation

Keywords

spinal cord; radiation myelopathy; prostacyclin; histopathology; rat

About this article
Title

Long-term changes of prostacyclin secretion in radiation-induced myelopathy

Journal

Reports of Practical Oncology and Radiotherapy

Issue

Vol 11, No 6 (2006)

Pages

273-279

Published online

2006-01-01

DOI

10.1016/S1507-1367(06)71072-0

Bibliographic record

Rep Pract Oncol Radiother 2006;11(6):273-279.

Keywords

spinal cord
radiation myelopathy
prostacyclin
histopathology
rat

Authors

Seied Rabie Mahdavi
Alireza Shirazi
Bagher Minaee
Alireza Nikoofar
Hamid Reza Mirzaee

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