open access
Effect of depth on radiation-induced cell damage in a water phantom
open access
Abstract
Aim
The paper describes the effect of medium depth on radiation-induced genetic damage to SK-mel cultured cells placed inside a water phantom as measured by the number of cells with micronuclei formed and apoptoses seen in these cells.
Materials/Methods
The energy of photon radiation used was 6 MV; cells placed at various depths were irradiated with the same dose (5 Gy).
Results/Conclusions
Depending on medium depth, differences were noted in the number of damaged cells: ca.30% more affected cells were seen at a depth of 25 cm as compared to a depth of 5 cm. With increasing depth also the radiation energy spectrum changes. This is probably responsible for the observed effect. It should be taken into consideration in both clinical practice and in treatment planning.
Abstract
Aim
The paper describes the effect of medium depth on radiation-induced genetic damage to SK-mel cultured cells placed inside a water phantom as measured by the number of cells with micronuclei formed and apoptoses seen in these cells.
Materials/Methods
The energy of photon radiation used was 6 MV; cells placed at various depths were irradiated with the same dose (5 Gy).
Results/Conclusions
Depending on medium depth, differences were noted in the number of damaged cells: ca.30% more affected cells were seen at a depth of 25 cm as compared to a depth of 5 cm. With increasing depth also the radiation energy spectrum changes. This is probably responsible for the observed effect. It should be taken into consideration in both clinical practice and in treatment planning.
Keywords
scattered radiation; energy spectrum; cytogenetic damage
About this articleTitle
Effect of depth on radiation-induced cell damage in a water phantom
Journal
Reports of Practical Oncology and Radiotherapy
Issue
Pages
37-41
Published online
2005-01-01
DOI
10.1016/S1507-1367(05)71080-4
Bibliographic record
Rep Pract Oncol Radiother 2005;10(1):37-41.
Keywords
scattered radiation
energy spectrum
cytogenetic damage
Authors
Krzysztof Ślosarek
Maria Konopacka
Jacek Rogoliński
Małgorzata Latocha
Aleksander Sochanik