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The role of collagen in co-cultures of human normal corneal and conjunctival cells
, 2, 3, 2, 4, 25
Affiliations- Department of Virology and Immunology, Institute of Microbiology and Biotechnology, Maria Curie-Skłodowska University, Lublin, Poland
- Department of General Ophthalmology, Medical University, Lublin, Poland
- Department of Human Anatomy, Medical University, Lublin, Poland
- Department of Ophthalmology, Rostock University Medical Centre, Rostock, Germany
- Medical Research Centre, Polish Academy of Science, Warsaw, Poland
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Abstract
Background: Intermediate interactions between corneal and conjunctival epithelial cells play an important role
in the process of correct vision. The goal of this paper was to establish whether the presence or absence of collagen
type I changes paracrine interactions between corneal and conjunctival epithelial cells.
Material and methods: Cultures of human corneal and conjunctival epithelial cells were used in the study. The
ELISA quantitative analysis of interleukin 1β (IL-1β), interleukin 6 (IL-6), urokinase-type plasminogen activator
(uPA), and uPA receptor (uPAR), assessment of the type of interactions between cells, as well as correlations between
tested parameters were performed.
Results: The presence of collagen type I changed the quantitative production of IL-1β and IL-6 by the examined
cells in the co-culture system. It did not affect the level of released uPA and uPAR. The presence or absence of
collagen also changed the relationship between the cells, which were evaluated in relation to changes in the level of
released cytokines.
Conclusions: Different levels of collagen type I constituting a component of extracellular matrix proteins significantly
affect and regulate the indirect interactions between human corneal and conjunctival epithelial cells.
Abstract
Background: Intermediate interactions between corneal and conjunctival epithelial cells play an important role
in the process of correct vision. The goal of this paper was to establish whether the presence or absence of collagen
type I changes paracrine interactions between corneal and conjunctival epithelial cells.
Material and methods: Cultures of human corneal and conjunctival epithelial cells were used in the study. The
ELISA quantitative analysis of interleukin 1β (IL-1β), interleukin 6 (IL-6), urokinase-type plasminogen activator
(uPA), and uPA receptor (uPAR), assessment of the type of interactions between cells, as well as correlations between
tested parameters were performed.
Results: The presence of collagen type I changed the quantitative production of IL-1β and IL-6 by the examined
cells in the co-culture system. It did not affect the level of released uPA and uPAR. The presence or absence of
collagen also changed the relationship between the cells, which were evaluated in relation to changes in the level of
released cytokines.
Conclusions: Different levels of collagen type I constituting a component of extracellular matrix proteins significantly
affect and regulate the indirect interactions between human corneal and conjunctival epithelial cells.
Keywords
corneal epithelial cells; conjunctival epithelial cells; co-culture; cytokines; serine protease uPA; uPAR; collagen
About this articleTitle
The role of collagen in co-cultures of human normal corneal and conjunctival cells
Journal
Issue
Vol 4 (2019): Continuous Publishing
Article type
Original paper
Pages
77-85
Published online
2019-11-13
Page views
606
Article views/downloads
654
DOI
Bibliographic record
Ophthalmol J 2019;4:77-85.
Keywords
corneal epithelial cells
conjunctival epithelial cells
co-culture
cytokines
serine protease uPA
uPAR
collagen
Authors
Roman Paduch
Anna Matysik-Woźniak
Ryszard Maciejewski
Beata Flis
Anselm G. Jünemann
Robert Rejdak
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