Vol 4 (2019): Continuous Publishing
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Published online: 2019-11-13

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The role of collagen in co-cultures of human normal corneal and conjunctival cells

Roman Paduch12, Anna Matysik-Woźniak2, Ryszard Maciejewski3, Beata Flis2, Anselm G. Jünemann4, Robert Rejdak25
Ophthalmol J 2019;4:77-85.

Abstract

Background: Intermediate interactions between corneal and conjunctival epithelial cells play an important role
in the process of correct vision. The goal of this paper was to establish whether the presence or absence of collagen
type I changes paracrine interactions between corneal and conjunctival epithelial cells.

Material and methods: Cultures of human corneal and conjunctival epithelial cells were used in the study. The
ELISA quantitative analysis of interleukin 1β (IL-1β), interleukin 6 (IL-6), urokinase-type plasminogen activator
(uPA), and uPA receptor (uPAR), assessment of the type of interactions between cells, as well as correlations between
tested parameters were performed.

Results: The presence of collagen type I changed the quantitative production of IL-1β and IL-6 by the examined
cells in the co-culture system. It did not affect the level of released uPA and uPAR. The presence or absence of
collagen also changed the relationship between the cells, which were evaluated in relation to changes in the level of
released cytokines.

Conclusions: Different levels of collagen type I constituting a component of extracellular matrix proteins significantly
affect and regulate the indirect interactions between human corneal and conjunctival epithelial cells.

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References

  1. Fukuda K, Nishida T. Reciprocal interaction of the conjunctiva and cornea in ocular allergy. J Allergy Clin Immunol. 2010; 125(2): 493–496.e2.
  2. Song Y, Kim JS, Choi EK, et al. TGF-β-independent CTGF induction regulates cell adhesion mediated drug resistance by increasing collagen I in HCC. Oncotarget. 2017; 8(13): 21650–21662.
  3. Sugioka K, Mishima H, Kodama A, et al. Regulatory Mechanism of Collagen Degradation by Keratocytes and Corneal Inflammation: The Role of Urokinase-Type Plasminogen Activator. Cornea. 2016; 35 Suppl 1: S59–S64.
  4. Lee JG, Heur M. Interleukin-1β enhances cell migration through AP-1 and NF-κB pathway-dependent FGF2 expression in human corneal endothelial cells. Biol Cell. 2013; 105(4): 175–189.
  5. Higuchi A, Kawakita T, Tsubota K. IL-6 induction in desiccated corneal epithelium in vitro and in vivo. Mol Vis. 2011; 17: 2400–2406.
  6. Wang Z, Sosne G, Kurpakus-Wheater M. Plasminogen activator inhibitor-1 (PAI-1) stimulates human corneal epithelial cell adhesion and migration in vitro. Exp Eye Res. 2005; 80(1): 1–8.
  7. Kerry DW, Hamilton-Miller JM, Brumfitt W. Trimethoprim and rifampicin: in vitro activities separately and in combination. J Antimicrob Chemother. 1975; 1(4): 417–427.
  8. Irkeç M, Bozkurt B. Epithelial cells in ocular allergy. Curr Allergy Asthma Rep. 2003; 3(4): 352–357.
  9. Dziasko MA, Daniels JT. Anatomical Features and Cell-Cell Interactions in the Human Limbal Epithelial Stem Cell Niche. Ocul Surf. 2016; 14(3): 322–330.
  10. Chang JH, Huang YH, Cunningham CM, et al. Matrix metalloproteinase 14 modulates signal transduction and angiogenesis in the cornea. Surv Ophthalmol. 2016; 61(4): 478–497.
  11. Park HY, Kim JH, Lee KM, et al. Effect of prostaglandin analogues on tear proteomics and expression of cytokines and matrix metalloproteinases in the conjunctiva and cornea. Exp Eye Res. 2012; 94(1): 13–21.
  12. Fukuda K, Fujitsu Y, Kumagai N, et al. Inhibition of matrix metalloproteinase-3 synthesis in human conjunctival fibroblasts by interleukin-4 or interleukin-13. Invest Ophthalmol Vis Sci. 2006; 47(7): 2857–2864.
  13. Bian F, Pelegrino FSA, Pflugfelder SC, et al. Desiccating Stress-Induced MMP Production and Activity Worsens Wound Healing in Alkali-Burned Corneas. Invest Ophthalmol Vis Sci. 2015; 56(8): 4908–4918.
  14. Heur M, Chaurasia SS, Wilson SE. Expression of interleukin-1 receptor antagonist in human cornea. Exp Eye Res. 2009; 88(5): 992–994.
  15. Stapleton WM, Chaurasia SS, Medeiros FW, et al. Topical interleukin-1 receptor antagonist inhibits inflammatory cell infiltration into the cornea. Exp Eye Res. 2008; 86(5): 753–757.
  16. Ebihara N, Matsuda A, Nakamura S, et al. Role of the IL-6 classic- and trans-signaling pathways in corneal sterile inflammation and wound healing. Invest Ophthalmol Vis Sci. 2011; 52(12): 8549–8557.
  17. Xi X, McMillan DH, Lehmann GM, et al. Ocular fibroblast diversity: implications for inflammation and ocular wound healing. Invest Ophthalmol Vis Sci. 2011; 52(7): 4859–4865.
  18. Sakimoto T, Sugaya S, Ishimori A, et al. Anti-inflammatory effect of IL-6 receptor blockade in corneal alkali burn. Exp Eye Res. 2012; 97(1): 98–104.
  19. Iwatake A, Murakami A, Ebihara N. The expression of matrix metalloproteinases and their inhibitors in corneal fibroblasts by alarmins from necrotic corneal epithelial cells. Jpn J Ophthalmol. 2018; 62(1): 92–100.
  20. Sugaya S, Sakimoto T, Shoji J, et al. Regulation of soluble interleukin-6 (IL-6) receptor release from corneal epithelial cells and its role in the ocular surface. Jpn J Ophthalmol. 2011; 55(3): 277–282.
  21. Xiang MH, Zhang XR, Zhang ZY, et al. Expressions of matrix metalloproteinases 1 and 3 and their tissue inhibitors in the conjunctival tissue and fibroblasts cultured from conjunctivochalasis. Int J Ophthalmol. 2017; 10(4): 555–559.
  22. Watanabe M, Yano W, Kondo S, et al. Up-regulation of urokinase-type plasminogen activator in corneal epithelial cells induced by wounding. Invest Ophthalmol Vis Sci. 2003; 44(8): 3332–3338.
  23. Sugioka K, Kodama A, Yoshida K, et al. The roles of urokinase-type plasminogen activator in leukocyte infiltration and inflammatory responses in mice corneas treated with lipopolysaccharide. Invest Ophthalmol Vis Sci. 2014; 55(8): 5338–5350.
  24. Swamynathan S, Swamynathan SK. SLURP-1 modulates corneal homeostasis by serving as a soluble scavenger of urokinase-type plasminogen activator. Invest Ophthalmol Vis Sci. 2014; 55(10): 6251–6261.