Vol 4 (2019): Continuous Publishing
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Published online: 2019-11-13

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The role of collagen in co-cultures of human normal corneal and conjunctival cells

Roman Paduch12, Anna Matysik-Woźniak2, Ryszard Maciejewski3, Beata Flis2, Anselm G. Jünemann4, Robert Rejdak25
Ophthalmol J 2019;4:77-85.


Background: Intermediate interactions between corneal and conjunctival epithelial cells play an important role
in the process of correct vision. The goal of this paper was to establish whether the presence or absence of collagen
type I changes paracrine interactions between corneal and conjunctival epithelial cells.

Material and methods: Cultures of human corneal and conjunctival epithelial cells were used in the study. The
ELISA quantitative analysis of interleukin 1β (IL-1β), interleukin 6 (IL-6), urokinase-type plasminogen activator
(uPA), and uPA receptor (uPAR), assessment of the type of interactions between cells, as well as correlations between
tested parameters were performed.

Results: The presence of collagen type I changed the quantitative production of IL-1β and IL-6 by the examined
cells in the co-culture system. It did not affect the level of released uPA and uPAR. The presence or absence of
collagen also changed the relationship between the cells, which were evaluated in relation to changes in the level of
released cytokines.

Conclusions: Different levels of collagen type I constituting a component of extracellular matrix proteins significantly
affect and regulate the indirect interactions between human corneal and conjunctival epithelial cells.

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