Vol 53, No 3 (2019)
Research paper
Published online: 2019-05-30
Submitted: 2019-01-29
Accepted: 2019-05-06
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Association between polymorphisms of a folate – homocysteine – methionine – SAM metabolising enzyme gene and multiple sclerosis in a Polish population

Monika Chorąży, Natalia Wawrusiewicz-Kurylonek, Joanna Gościk, Renata Posmyk, Agata Czarnowska, Marta Więsik, Katarzyna Kapica-Topczewska, Adam Jacek Krętowski, Jan Kochanowicz, Alina Kułakowska
DOI: 10.5603/PJNNS.a2019.0019
·
Pubmed: 31145465
·
Neurol Neurochir Pol 2019;53(3):194-198.

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Vol 53, No 3 (2019)
Research paper
Published online: 2019-05-30
Submitted: 2019-01-29
Accepted: 2019-05-06

Abstract

Background and Objectives. Multiple sclerosis (MS) is a chronic inflammatory, autoimmune disease with a still unknown aetiology. The main initial mechanism of demyelination and injury to the central nervous system (CNS) appears to be inflammation. Neurotoxicity induced by homocysteine (Hcy) may be a factor affecting this process. 5,10-methylenetetrahydrofolate reductase (MTHFR) is an essential enzyme involved in Hcy metabolism. It leads to Hcy remethylation to methionine. In the present study, we aimed to investigate a possible association between two variants of MTHFR gene in patients with MS in Poland and healthy individuals.

Methods. In this study, we genotyped 174 relapsing-remitting MS patients and 186 healthy controls using the TaqMan technique.

Results and Conclusions. It was found that, regardless of the presence of a specific allele, the gender of MS patients affects age at the time of the clinical onset of the disease: in rs1801133 for the C allele and T, the average age was 35 years for women and 29 for men (p = 0.0004; p = 0.034 respectively). Similarly for the second polymorphism rs1801131 for the A allele and C, the average age was 35 years for women and 29 for men (p = 0.001; p = 0.01 respectively). No significant allelic / genotypic frequency differences have been observed between the studied groups (c.677C > T, CT/TT p = 0.719, p = 0.262; c.1298A > C, AC/CC of p = 0.686; p = 0.66). We found no association between polymorphisms of a folate-homocysteine-methionine-SAM metabolising gene enzyme and multiple sclerosis in a Polish population.

Abstract

Background and Objectives. Multiple sclerosis (MS) is a chronic inflammatory, autoimmune disease with a still unknown aetiology. The main initial mechanism of demyelination and injury to the central nervous system (CNS) appears to be inflammation. Neurotoxicity induced by homocysteine (Hcy) may be a factor affecting this process. 5,10-methylenetetrahydrofolate reductase (MTHFR) is an essential enzyme involved in Hcy metabolism. It leads to Hcy remethylation to methionine. In the present study, we aimed to investigate a possible association between two variants of MTHFR gene in patients with MS in Poland and healthy individuals.

Methods. In this study, we genotyped 174 relapsing-remitting MS patients and 186 healthy controls using the TaqMan technique.

Results and Conclusions. It was found that, regardless of the presence of a specific allele, the gender of MS patients affects age at the time of the clinical onset of the disease: in rs1801133 for the C allele and T, the average age was 35 years for women and 29 for men (p = 0.0004; p = 0.034 respectively). Similarly for the second polymorphism rs1801131 for the A allele and C, the average age was 35 years for women and 29 for men (p = 0.001; p = 0.01 respectively). No significant allelic / genotypic frequency differences have been observed between the studied groups (c.677C > T, CT/TT p = 0.719, p = 0.262; c.1298A > C, AC/CC of p = 0.686; p = 0.66). We found no association between polymorphisms of a folate-homocysteine-methionine-SAM metabolising gene enzyme and multiple sclerosis in a Polish population.

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Keywords

multiple sclerosis, polymorphism, MTHFR gene, folate

About this article
Title

Association between polymorphisms of a folate – homocysteine – methionine – SAM metabolising enzyme gene and multiple sclerosis in a Polish population

Journal

Neurologia i Neurochirurgia Polska

Issue

Vol 53, No 3 (2019)

Pages

194-198

Published online

2019-05-30

DOI

10.5603/PJNNS.a2019.0019

Pubmed

31145465

Bibliographic record

Neurol Neurochir Pol 2019;53(3):194-198.

Keywords

multiple sclerosis
polymorphism
MTHFR gene
folate

Authors

Monika Chorąży
Natalia Wawrusiewicz-Kurylonek
Joanna Gościk
Renata Posmyk
Agata Czarnowska
Marta Więsik
Katarzyna Kapica-Topczewska
Adam Jacek Krętowski
Jan Kochanowicz
Alina Kułakowska

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