open access

Vol 50, No 3 (2016)
Original research articles
Submitted: 2015-12-03
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The impact of multiple sclerosis relapse treatment on migration of effector T cells – Preliminary study

Izabela Jatczak-Pawlik, Dominika Książek-Winiarek, Dagmara Wojkowska, Krzysztof Jóźwiak, Karol Jastrzębski, Mirosława Pietruczuk, Andrzej Głąbiński
DOI: 10.1016/j.pjnns.2016.02.003
·
Neurol Neurochir Pol 2016;50(3):155-162.

open access

Vol 50, No 3 (2016)
Original research articles
Submitted: 2015-12-03

Abstract

Migration of inflammatory cells from the blood to the central nervous system (CNS) is crucial for development of multiple sclerosis (MS). Inhibition of this process would allow to control disease activity. The first step confirming this approach would be the analysis of the impact of effective MS relapse therapy on migration of effector T cells. The aim of the study was to analyze the influence of methylprednisolone (MP) on the migratory activity of effector CD4+ T cells from MS patients. Moreover, to study the potential mechanism of this process we studied expression of chemokine receptors on migrating cells.

Material and methods

Peripheral blood samples were obtained from relapsing-remitting MS (RR-MS) patients during relapse (n=23) and from control group (n=23). After isolation CD4+ T cells were incubated with various concentrations of MP. Then they were stimulated in chemotaxis assay with chemokines CCL3 or CXCL10 or were used to CCR1 and CXCR3 expression analysis.

Results

CXCL10- and CCL3-stimulated migration of CD4+ T cells was significantly increased in MS. MP was able to reduce in vitro migration of effector T cells induced by CXCL10, but not by CCL3. Inhibition by MP was dose-dependent. Expression of analyzed chemokine receptors was unaltered after MP incubation.

Conclusions

MP reduced CD4+ T cells migration induced by CXCL10 without affecting CXCR3 expression. These observations demonstrate one of the potential mechanisms of MP action in MS, distinct from inducing cell apoptosis, and suggests the new targets for development of more effective MS treatments.

Abstract

Migration of inflammatory cells from the blood to the central nervous system (CNS) is crucial for development of multiple sclerosis (MS). Inhibition of this process would allow to control disease activity. The first step confirming this approach would be the analysis of the impact of effective MS relapse therapy on migration of effector T cells. The aim of the study was to analyze the influence of methylprednisolone (MP) on the migratory activity of effector CD4+ T cells from MS patients. Moreover, to study the potential mechanism of this process we studied expression of chemokine receptors on migrating cells.

Material and methods

Peripheral blood samples were obtained from relapsing-remitting MS (RR-MS) patients during relapse (n=23) and from control group (n=23). After isolation CD4+ T cells were incubated with various concentrations of MP. Then they were stimulated in chemotaxis assay with chemokines CCL3 or CXCL10 or were used to CCR1 and CXCR3 expression analysis.

Results

CXCL10- and CCL3-stimulated migration of CD4+ T cells was significantly increased in MS. MP was able to reduce in vitro migration of effector T cells induced by CXCL10, but not by CCL3. Inhibition by MP was dose-dependent. Expression of analyzed chemokine receptors was unaltered after MP incubation.

Conclusions

MP reduced CD4+ T cells migration induced by CXCL10 without affecting CXCR3 expression. These observations demonstrate one of the potential mechanisms of MP action in MS, distinct from inducing cell apoptosis, and suggests the new targets for development of more effective MS treatments.

Get Citation

Keywords

Methylprednisolone, Multiple sclerosis, Migration, Chemokines, Chemokine receptors

About this article
Title

The impact of multiple sclerosis relapse treatment on migration of effector T cells – Preliminary study

Journal

Neurologia i Neurochirurgia Polska

Issue

Vol 50, No 3 (2016)

Pages

155-162

DOI

10.1016/j.pjnns.2016.02.003

Bibliographic record

Neurol Neurochir Pol 2016;50(3):155-162.

Keywords

Methylprednisolone
Multiple sclerosis
Migration
Chemokines
Chemokine receptors

Authors

Izabela Jatczak-Pawlik
Dominika Książek-Winiarek
Dagmara Wojkowska
Krzysztof Jóźwiak
Karol Jastrzębski
Mirosława Pietruczuk
Andrzej Głąbiński

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