open access

Vol 47, No 2 (2013)
ARTYKUŁ ORYGINALNY
Submitted: 2012-01-05
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The effects of fetal allogeneic umbilical cord tissue transplant following experimental spinal cord injury on urinary bladder morphology

Bulent Erdogan, Ozgur Yaycioglu, Iffet Feride Sahin, Fazilet Kayaselcuk, Berker Cemil, Emre Cemal Gokce, Murad Bavbek
DOI: 10.5114/ninp.2013.33824
·
Neurol Neurochir Pol 2013;47(2):138-144.

open access

Vol 47, No 2 (2013)
ARTYKUŁ ORYGINALNY
Submitted: 2012-01-05

Abstract

Background and purpose

In continuation of our previous experimental study on spinal cord injury (SCI) using fetal stem cells, we investigated here the effects of fetal allogeneic umbilical cord tissue transplant on the urinary bladder morphology in a rat SCI model.

Material and methods

Five pregnant albino Wistar rats at 12 days of gestation were used to obtain the umbilical cord cell graft. In Group 1 (n = 5), Th8-Th9 laminectomy was performed. Group 2 (n = 5) received spinal cord injury. In Group 3 (n = 5), the cultured fetal umbilical cord cells coated with alginate gel were placed into the lesion cavity. In Group 4 (n = 5), only alginate sponges without umbilical cord cells were placed into the injury cavity. The bladders of animals were analyzed pathologically at 21 days after surgery.

Results

The thickness of the epithelium and the lamina propria did not differ among studied groups (p > 0.05). The lamina muscularis thickness was significantly higher in Group 2 and Group 4 than the others (p < 0.05). The bladder weight was similar among Groups 1, 2, and 3 (p > 0.05). Fibrosis was significantly increased in Group 2 (p < 0.05); it was greater in Group 2 than in Group 3 (p < 0.05) but did not differ between Groups 1 and 3 (p > 0.05).

Conclusions

This study suggests that allogeneic umbilical cord tissue transplantation after SCI may prevent bladder wall hypertrophy and fibrosis in the rat SCI model.

Abstract

Background and purpose

In continuation of our previous experimental study on spinal cord injury (SCI) using fetal stem cells, we investigated here the effects of fetal allogeneic umbilical cord tissue transplant on the urinary bladder morphology in a rat SCI model.

Material and methods

Five pregnant albino Wistar rats at 12 days of gestation were used to obtain the umbilical cord cell graft. In Group 1 (n = 5), Th8-Th9 laminectomy was performed. Group 2 (n = 5) received spinal cord injury. In Group 3 (n = 5), the cultured fetal umbilical cord cells coated with alginate gel were placed into the lesion cavity. In Group 4 (n = 5), only alginate sponges without umbilical cord cells were placed into the injury cavity. The bladders of animals were analyzed pathologically at 21 days after surgery.

Results

The thickness of the epithelium and the lamina propria did not differ among studied groups (p > 0.05). The lamina muscularis thickness was significantly higher in Group 2 and Group 4 than the others (p < 0.05). The bladder weight was similar among Groups 1, 2, and 3 (p > 0.05). Fibrosis was significantly increased in Group 2 (p < 0.05); it was greater in Group 2 than in Group 3 (p < 0.05) but did not differ between Groups 1 and 3 (p > 0.05).

Conclusions

This study suggests that allogeneic umbilical cord tissue transplantation after SCI may prevent bladder wall hypertrophy and fibrosis in the rat SCI model.

Get Citation

Keywords

neurogenic, spinal cord injuries, stem cell transplantation, umbilical, urinary bladder

About this article
Title

The effects of fetal allogeneic umbilical cord tissue transplant following experimental spinal cord injury on urinary bladder morphology

Journal

Neurologia i Neurochirurgia Polska

Issue

Vol 47, No 2 (2013)

Pages

138-144

DOI

10.5114/ninp.2013.33824

Bibliographic record

Neurol Neurochir Pol 2013;47(2):138-144.

Keywords

neurogenic
spinal cord injuries
stem cell transplantation
umbilical
urinary bladder

Authors

Bulent Erdogan
Ozgur Yaycioglu
Iffet Feride Sahin
Fazilet Kayaselcuk
Berker Cemil
Emre Cemal Gokce
Murad Bavbek

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