open access

Vol 46, No 1 (2012)
ARTYKUŁ ORYGINALNY
Submitted: 2011-01-19
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Evaluation of the relationship between leptin, resistin, adiponectin and natural regulatory T cells in relapsing-remitting multiple sclerosis

Łukasz Kraszula, Anna Jasińska, Makandjou-Ola Eusebio, Piotr Kuna, Andrzej Głąbiński, Mirosława Pietruczuk
DOI: 10.5114/ninp.2012.27211
·
Neurol Neurochir Pol 2012;46(1):22-28.

open access

Vol 46, No 1 (2012)
ARTYKUŁ ORYGINALNY
Submitted: 2011-01-19

Abstract

Background and purpose

Data suggest that adipocytokines and natural regulatory T (nTreg) cells play a pivotal role in the immunopathogenesis of multiple sclerosis and the associated inflammation. The purpose of this study was to evaluate selected adipocytokines and nTreg cells and to assess their relationship with relapsing-remitting multiple sclerosis (RRMS).

Material and methods

The study was conducted among 25 patients with RRMS and 25 healthy individuals. Blood samples were collected within two weeks after the beginning of acute relapse of RRMS. The body mass index (BMI) of each patient was calculated. Serum adipocytokine concentrations were determined by ELISA and nTreg cells were evaluated using multicolour flow cytometry.

Results

Patients and controls had similar BMI, regardless of gender. Significantly higher leptin and resistin levels and significantly lower adiponectin levels were found in patients with RRMS in comparison to the control group (p < 0.0001). The percentage of nTreg cells (p < 0.01) and the mean fluorescence channel (MFC) of FoxP3 were significantly reduced in patients with RRMS (p < 0.001). There was an inverse correlation between leptin concentration and MFC of the transcription factor Foxp3 nTreg in patients with RRMS (r = −0.7, p < 0.05).

Conclusions

Proinflammatory adipocytokine profile and decreased percentage of nTreg cells suggest their implication in the inflammatory response in RRMS regardless of corticosteroid therapy. The correlation between leptin and the MFC of the transcription factor Foxp3 in nTreg cells in patients with RRMS suggests its inhibitory effect on FoxP3 expression.

Abstract

Background and purpose

Data suggest that adipocytokines and natural regulatory T (nTreg) cells play a pivotal role in the immunopathogenesis of multiple sclerosis and the associated inflammation. The purpose of this study was to evaluate selected adipocytokines and nTreg cells and to assess their relationship with relapsing-remitting multiple sclerosis (RRMS).

Material and methods

The study was conducted among 25 patients with RRMS and 25 healthy individuals. Blood samples were collected within two weeks after the beginning of acute relapse of RRMS. The body mass index (BMI) of each patient was calculated. Serum adipocytokine concentrations were determined by ELISA and nTreg cells were evaluated using multicolour flow cytometry.

Results

Patients and controls had similar BMI, regardless of gender. Significantly higher leptin and resistin levels and significantly lower adiponectin levels were found in patients with RRMS in comparison to the control group (p < 0.0001). The percentage of nTreg cells (p < 0.01) and the mean fluorescence channel (MFC) of FoxP3 were significantly reduced in patients with RRMS (p < 0.001). There was an inverse correlation between leptin concentration and MFC of the transcription factor Foxp3 nTreg in patients with RRMS (r = −0.7, p < 0.05).

Conclusions

Proinflammatory adipocytokine profile and decreased percentage of nTreg cells suggest their implication in the inflammatory response in RRMS regardless of corticosteroid therapy. The correlation between leptin and the MFC of the transcription factor Foxp3 in nTreg cells in patients with RRMS suggests its inhibitory effect on FoxP3 expression.

Get Citation

Keywords

multiple sclerosis, leptin, resistin, adiponectin, natural regulatory T cells (nTreg), FoxP3

About this article
Title

Evaluation of the relationship between leptin, resistin, adiponectin and natural regulatory T cells in relapsing-remitting multiple sclerosis

Journal

Neurologia i Neurochirurgia Polska

Issue

Vol 46, No 1 (2012)

Pages

22-28

DOI

10.5114/ninp.2012.27211

Bibliographic record

Neurol Neurochir Pol 2012;46(1):22-28.

Keywords

multiple sclerosis
leptin
resistin
adiponectin
natural regulatory T cells (nTreg)
FoxP3

Authors

Łukasz Kraszula
Anna Jasińska
Makandjou-Ola Eusebio
Piotr Kuna
Andrzej Głąbiński
Mirosława Pietruczuk

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