open access
The double immunostaining of CD133 and Ki-67 favours a significant co-localization pattern in fibroblastic subtype of meningiomas
- department of neurosurgery
- Akdeniz University, Faculty of Medicine, Department of Histology and Embryology, Antalya, Turkey, Antalya, Türkiye
open access
Abstract
A unique molecular and/or cellular marker for meningiomas, the most common intracranial tumours, has not been identified yet.
Material and methodsWe investigated the co-localization fraction of CD133/Ki-67 in meningioma tissue array slide composed of 80 meningioma tissue samples of various histological variants. CD133 – a cell membrane stem cell marker – was previously proved to be associated with the initiation and progression of intracerebral gliomas and medulloblastomas.
ResultsImmunohistochemical co-localization of CD133/Ki-67 was significantly higher in fibroblastic variant than in meningothelial and transitional subtypes. However, since there were only 3 atypical and 1 malignant meningioma spots in the tumour tissue array slide, it is difficult to draw a firm conclusion regarding the actual co-localization percentage and persistence of CD133/Ki-67 in atypical and malignant meningiomas.
ConclusionsFar higher co-staining percentage of CD133/Ki-67 in fibroblastic meningioma samples compared to meningothelial subtype, a histological meningioma variant, architectonically resembling the non-neoplastic meningeal cells, gave us the impression that CD133 may play a role in the formation and progression of fibroblastic meningioma variants. The persistency and the validity of this finding need to be verified by further histopathological and molecular research in order to clarify the possible role of CD133 in meningiogenesis.
Abstract
A unique molecular and/or cellular marker for meningiomas, the most common intracranial tumours, has not been identified yet.
Material and methodsWe investigated the co-localization fraction of CD133/Ki-67 in meningioma tissue array slide composed of 80 meningioma tissue samples of various histological variants. CD133 – a cell membrane stem cell marker – was previously proved to be associated with the initiation and progression of intracerebral gliomas and medulloblastomas.
ResultsImmunohistochemical co-localization of CD133/Ki-67 was significantly higher in fibroblastic variant than in meningothelial and transitional subtypes. However, since there were only 3 atypical and 1 malignant meningioma spots in the tumour tissue array slide, it is difficult to draw a firm conclusion regarding the actual co-localization percentage and persistence of CD133/Ki-67 in atypical and malignant meningiomas.
ConclusionsFar higher co-staining percentage of CD133/Ki-67 in fibroblastic meningioma samples compared to meningothelial subtype, a histological meningioma variant, architectonically resembling the non-neoplastic meningeal cells, gave us the impression that CD133 may play a role in the formation and progression of fibroblastic meningioma variants. The persistency and the validity of this finding need to be verified by further histopathological and molecular research in order to clarify the possible role of CD133 in meningiogenesis.
Keywords
meningioma, fibroblastic, CD133, Ki-67, tissue array, co-localization
Title
The double immunostaining of CD133 and Ki-67 favours a significant co-localization pattern in fibroblastic subtype of meningiomas
Journal
Neurologia i Neurochirurgia Polska
Issue
Pages
467-473
Page views
313
Article views/downloads
535
DOI
10.1016/S0028-3843(14)60315-7
Bibliographic record
Neurol Neurochir Pol 2011;45(5):467-473.
Keywords
meningioma
fibroblastic
CD133
Ki-67
tissue array
co-localization
Authors
Baki S. Albayrak
Ozgur Ismailoglu
Gamze Tanriover
Eralp N. Cetinalp
Necdet Demir