open access

Vol 10, No 4 (2017)
ORIGINAL PAPERS
Published online: 2018-01-17
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Reveos system - an Alternative in Pooled Platelet Concentrate Production

Paweł Szykuła, Kamil Stawicki, Dioniza Marciniak-Bielak
Journal of Transfusion Medicine 2017;10(4):130-137.

open access

Vol 10, No 4 (2017)
ORIGINAL PAPERS
Published online: 2018-01-17

Abstract

Introduction: Due to broad application of platelet concentrates in medicine there is growing interest in those products among specialists in transfusion medicine, employees of the Regional Blood Transfusion Centres as well as institutions that develop and implement new methods of obtaining platelet concentrates. The objective of this work is to present the quantitative and qualitative parameters of leukoreduced pooled platelet concentrates obtained from interim platelet units (IPU) by use of the Reveos system and their comparison with products obtained from buffy coats manually and by use of the OrbiSac system. Material and methods: Each of the 3 methods was used to obtain 12 platelet concentrates in plasma and 12 platelet concentrates in platelet additive solution (PAS). Platelet concentrates were obtained, depending on the method, using 5 buffy coats or 5 IPU. The parameters addressed were: volume, platelet count, leukocyte count and pH value of the platelet concentrates obtained. Results: The value of buffy coat haematocrit was 55.3 ± 3.8%. Their average volume was over 60% higher than the average IPU volume. There were no statistically significant differences in the platelet count between buffy coats and IPU, both in the case of measured values and values estimated by Reveos. The correlation coefficient (0.91) points to a strong positive correlation between device indications and the determined platelet count. After filtration all the products contained less than 1 × 106 of leucocytes/unit. They may be qualified as leukoreduced products. pH on the fifth day of platelet concentrates storage in every product was between 7.1–7.4. Conclusions: LPC (leukocytereduced platelet concentrate) prepared with the use of IPU from Reveos are not different from products prepared by use of other methods. The Reveos system is a good alternative for the standard methods of LPC production, and moreover, with the use of IPU selection, the preparation process is improved.

Abstract

Introduction: Due to broad application of platelet concentrates in medicine there is growing interest in those products among specialists in transfusion medicine, employees of the Regional Blood Transfusion Centres as well as institutions that develop and implement new methods of obtaining platelet concentrates. The objective of this work is to present the quantitative and qualitative parameters of leukoreduced pooled platelet concentrates obtained from interim platelet units (IPU) by use of the Reveos system and their comparison with products obtained from buffy coats manually and by use of the OrbiSac system. Material and methods: Each of the 3 methods was used to obtain 12 platelet concentrates in plasma and 12 platelet concentrates in platelet additive solution (PAS). Platelet concentrates were obtained, depending on the method, using 5 buffy coats or 5 IPU. The parameters addressed were: volume, platelet count, leukocyte count and pH value of the platelet concentrates obtained. Results: The value of buffy coat haematocrit was 55.3 ± 3.8%. Their average volume was over 60% higher than the average IPU volume. There were no statistically significant differences in the platelet count between buffy coats and IPU, both in the case of measured values and values estimated by Reveos. The correlation coefficient (0.91) points to a strong positive correlation between device indications and the determined platelet count. After filtration all the products contained less than 1 × 106 of leucocytes/unit. They may be qualified as leukoreduced products. pH on the fifth day of platelet concentrates storage in every product was between 7.1–7.4. Conclusions: LPC (leukocytereduced platelet concentrate) prepared with the use of IPU from Reveos are not different from products prepared by use of other methods. The Reveos system is a good alternative for the standard methods of LPC production, and moreover, with the use of IPU selection, the preparation process is improved.
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Keywords

platelet concentrate; Reveos; interim platelet unit; automation

About this article
Title

Reveos system - an Alternative in Pooled Platelet Concentrate Production

Journal

Journal of Transfusion Medicine

Issue

Vol 10, No 4 (2017)

Pages

130-137

Published online

2018-01-17

Bibliographic record

Journal of Transfusion Medicine 2017;10(4):130-137.

Keywords

platelet concentrate
Reveos
interim platelet unit
automation

Authors

Paweł Szykuła
Kamil Stawicki
Dioniza Marciniak-Bielak

References (14)
  1. Łętowska M, Rosiek A. Stosowanie komórkowych składników krwi w onkologii. Onkol Prakt Klin. 2006; 2(1): 6–17.
  2. Amable PR, Carias RB, Teixeira MV, et al. Platelet-rich plasma preparation for regenerative medicine: optimization and quantification of cytokines and growth factors. Stem Cell Res Ther. 2013; 4(3): 67.
  3. Dohan Ehrenfest DM, Rasmusson L, Albrektsson T. Classification of platelet concentrates: from pure platelet-rich plasma (P-PRP) to leucocyte- and platelet-rich fibrin (L-PRF). Trends Biotechnol. 2009; 27(3): 158–167.
  4. Jameson C. Autologous Platelet Concentrate for the Production of Platelet Gel. Laboratory Medicine. 2007; 38(1): 39–42.
  5. Janetzko K, Klüter H, van Waeg G, et al. Fully automated processing of buffy-coat-derived pooled platelet concentrates. Transfusion. 2004; 44(7): 1052–1058.
  6. Antoniewicz-Papis J, Lachert E, Rosiek A. Otrzymywanie metodą automatyczną zlewanych koncentratów krwinek płytkowych J. Transf Med. 2010; 2: 49–54.
  7. Lagerberg JW, Salado-Jimena JA, Löf H, et al. Evaluation of the quality of blood components obtained after automated separation of whole blood by a new multiunit processor. Transfusion. 2013; 53(8): 1798–1807.
  8. Chicchi R, Biguzzi R, Santarelli R. The OrbiSac system: results and organizational impact. Blood Transfus. 2007; 5(1): 15–19.
  9. Janetzko K, Klüter H, van Waeg G, et al. Fully automated processing of buffy-coat-derived pooled platelet concentrates. Transfusion. 2004; 44(7): 1052–1058.
  10. van der Meer PF. PAS or plasma for storage of platelets? A concise review. Transfus Med. 2016; 26(5): 339–342.
  11. Monge R, Perez V, Azakarte A, et al. Quality of blood component using the Reveos automatem blood processng system. Vox Sanguinis. 2013; 105: 11–12.
  12. Johnson L, Winter KM, Kwok M, et al. Evaluation of the quality of blood components prepared using the Reveos automated blood processing system. Vox Sang. 2013; 105(3): 225–235.
  13. Łętowska M. Medyczne zasady pobierania krwi, oddzielania jej składników i wydawania, obowiązujące w jednostkach organizacyjnych publicznej służby krwi. Wydanie III. ; 2014.
  14. Council of Europe: Guide to the preparation, use and quality assurance of blood components. Strasbourg, Council of Europe Press. ; 2008.

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