Hemoglobin level is the basic parameter qualifying for blood donation. Hemoglobin measurement is performed using automated hematology analyzers (complete blood count) or HemoCue-type analyzers designed to measure hemoglobin level only (HemoCue hemoglobin-meters). Hemoglobin measurements are routinely made from vein or capillary blood samples. A non-invasive method of hemoglobin measurement has recently become available with introduction of the Haemospect analyzer. It is a most welcome alternative to vein and capillary tests. Donors who have problems with tests on blood samples from fingerstick are more willing to return to blood establishments for subsequent blood donation. Measurements are based on spectroscopy. The aim of the study was to compare hemoglobin measurements obtained with three analyzers: Haemospect, Beckman Coulter and HemoCue In the first part of the study we compared the Hb measurements from Haemospect and Beckman Coulter hematology analyzers. Test results for men and women were analyzed separately as the Hb criteria for men and women donors are different (≥ 13,5 g / and ≥ 12.5 g/dl respectively). For men, the mean Hb level measured with Haemospect was 14,8 ± 0,9 g/dl while with hematology analyzer it was 15,3 ± 1,0 g/dl. For women, the mean Hb level measured with Haemospect was 13,3 ± 0,5 g/dl and 13,3 ± 0,9 g/dl with hematology analyzer. In the second part of the study we compared the Hb measurements from Haemospect (14,7 ± 1,2 g/dl) with results from Beckman Coulter hematology analyzer (14,5 ± 0,8 g/dl) and HemoCue analyzer (14,1 ± 1,2 g/dl). No statistically significant differences were observed between results obtained with Beckman Coulter hematology analyzer and HemoCue and Haemospect analyzers.