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Vol 1, No 1 (2008)
Research paper
Published online: 2009-02-02
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Biochemical changes in irradiated Red Blood Cells stored up to 42 days

Jolanta Kubis, Elżbieta Lachert, Jolanta Antoniewicz-Papis, Aleksandra Dzieciątkowska, Magdalena Łętowska
Journal of Transfusion Medicine 2008;1(1):46-54.

open access

Vol 1, No 1 (2008)
ORIGINAL PAPERS
Published online: 2009-02-02

Abstract

Introduction: Irradiation of whole blood and cellular components is currently the only effective method of preventing the severe complication of transfusion-associated graft-versus-host disease (TA-GvHD). The main indication for irradiated blood and blood components is for patients with congenital or acquired immunological deficiency, in cases of neonatal intrauterine and exchange transfusions as well as in close relationship between donor and recipient. Irradiation of blood and blood components results in the inactivation of T lymphocytes, more sensitive to irradiation than other therapeutically active cells such as erythrocytes, platelet concentrates and granulocytes. No optimal dose of gamma irradiation for Red Blood Cell Concentrates (RBCs) and Platelet Concentrates (PCs) has yet been determined. The aim of this study was to determine the effect of two different irradiation doses (25 Gy, 50 Gy) on the in vitro functions of RBCs as well as changes in irradiated RBCs stored in ADSOL for various periods of time (7 , 14, 28, 35, 42 days of storage at 4°C).
Material and methods: The study involved 45 units of RBCs in ADSOL. The following tests were performed to assess the quality of in vitro functions of RBCs: erythrocyte (RBC) and leukocyte (WBC) counts, hematocrit (Ht), supernatant hemoglobin (Hb), potassium( K+) and sodium concentration (Na+), adenosine-tri-phosphate concentration (ATP), 2,3-diphosphoglycerate (2,3-DPG) and pH.
Results: The statistically significant increase of K+ was determined in irradiated PCs (50 Gy) on all storage days. The same level of 2,3-DPG concentration was found in control group and RBCs irradiated with 25 Gy, while the 2,3-DPG concentration in groups irradiated with 50 Gy was only 0,04 mmol/ml.
Conclusion: Comparison of the basic biochemical parameters (APT level, 2,3-DPG, Hb, K+ concentration) of the 25 and 50 Gy irradiated RBCs with those of the unirradiated RBCs, gives grounds for conclusion that RBCs irradiated with 50 Gy should not be stored at 4°C for more than 14 days, whether in ADSOL or not, while RBCs irradiated with 25 Gy can be stored up to 28 days prior to transfusion. In vitro tests demonstrate that irradiated RBCs function well in the recipient’s circulatory system.

Abstract

Introduction: Irradiation of whole blood and cellular components is currently the only effective method of preventing the severe complication of transfusion-associated graft-versus-host disease (TA-GvHD). The main indication for irradiated blood and blood components is for patients with congenital or acquired immunological deficiency, in cases of neonatal intrauterine and exchange transfusions as well as in close relationship between donor and recipient. Irradiation of blood and blood components results in the inactivation of T lymphocytes, more sensitive to irradiation than other therapeutically active cells such as erythrocytes, platelet concentrates and granulocytes. No optimal dose of gamma irradiation for Red Blood Cell Concentrates (RBCs) and Platelet Concentrates (PCs) has yet been determined. The aim of this study was to determine the effect of two different irradiation doses (25 Gy, 50 Gy) on the in vitro functions of RBCs as well as changes in irradiated RBCs stored in ADSOL for various periods of time (7 , 14, 28, 35, 42 days of storage at 4°C).
Material and methods: The study involved 45 units of RBCs in ADSOL. The following tests were performed to assess the quality of in vitro functions of RBCs: erythrocyte (RBC) and leukocyte (WBC) counts, hematocrit (Ht), supernatant hemoglobin (Hb), potassium( K+) and sodium concentration (Na+), adenosine-tri-phosphate concentration (ATP), 2,3-diphosphoglycerate (2,3-DPG) and pH.
Results: The statistically significant increase of K+ was determined in irradiated PCs (50 Gy) on all storage days. The same level of 2,3-DPG concentration was found in control group and RBCs irradiated with 25 Gy, while the 2,3-DPG concentration in groups irradiated with 50 Gy was only 0,04 mmol/ml.
Conclusion: Comparison of the basic biochemical parameters (APT level, 2,3-DPG, Hb, K+ concentration) of the 25 and 50 Gy irradiated RBCs with those of the unirradiated RBCs, gives grounds for conclusion that RBCs irradiated with 50 Gy should not be stored at 4°C for more than 14 days, whether in ADSOL or not, while RBCs irradiated with 25 Gy can be stored up to 28 days prior to transfusion. In vitro tests demonstrate that irradiated RBCs function well in the recipient’s circulatory system.
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Keywords

red blood cells (RBCs); transfusion-associated graft-versus-host disease (TA-GvHD); irradiation

About this article
Title

Biochemical changes in irradiated Red Blood Cells stored up to 42 days

Journal

Journal of Transfusion Medicine

Issue

Vol 1, No 1 (2008)

Article type

Research paper

Pages

46-54

Published online

2009-02-02

Bibliographic record

Journal of Transfusion Medicine 2008;1(1):46-54.

Keywords

red blood cells (RBCs)
transfusion-associated graft-versus-host disease (TA-GvHD)
irradiation

Authors

Jolanta Kubis
Elżbieta Lachert
Jolanta Antoniewicz-Papis
Aleksandra Dzieciątkowska
Magdalena Łętowska

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