open access

Vol 1, No 1 (2008)
ORIGINAL PAPERS
Published online: 2009-02-02
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The frequency of pseudothrombocytopenia in blood donors from the central region of Poland

Dioniza Marciniak-Bielak, Bolesława Stróżowska, Anna Główka, Andrzej Szczepiński, Krystyna Maślanka
Journal of Transfusion Medicine 2008;1(1):36-39.

open access

Vol 1, No 1 (2008)
ORIGINAL PAPERS
Published online: 2009-02-02

Abstract

Background: Pseudothrombocytopenia (PTCP) is a phenomenon of falsely low platelet count associated with in vitro platelet agglutination due to EDTA-dependent anti-platelet antibodies. The aim of this study was to determine EDTA-dependent pseudothrombocytopenia frequency in blood donors and the characteristics of EDTA-dependent anti-platelet antibodies in PTCP donors.
Material and methods: Between January 2001 and August 2008, platelet count was routinely measured in 83.486 unselected Polish blood donors. EDTA-dependent anti-platelet antibodies were detected by using the platelet immunofluorescence test (PIFT).
Results: PTCP was diagnosed in 12 healthy donors. In EDTA samples, the average number of platelets was 66.8 × 109/l (SD ± 30.8 × 109/l ) and 209.9 × 109/l (SD ± 46.6 x 109/l) in citrate samples. EDTA-dependant IgG and IgM class antibodies were found in the sera of 10 donors. In 2 donors, PTCP diagnosis was based only on the differences between platelet counts in EDTA and in citrate samples.
Conclusion: The PTCP frequency in blood donors was calculated at 1:6957, that is 0.014%.

Abstract

Background: Pseudothrombocytopenia (PTCP) is a phenomenon of falsely low platelet count associated with in vitro platelet agglutination due to EDTA-dependent anti-platelet antibodies. The aim of this study was to determine EDTA-dependent pseudothrombocytopenia frequency in blood donors and the characteristics of EDTA-dependent anti-platelet antibodies in PTCP donors.
Material and methods: Between January 2001 and August 2008, platelet count was routinely measured in 83.486 unselected Polish blood donors. EDTA-dependent anti-platelet antibodies were detected by using the platelet immunofluorescence test (PIFT).
Results: PTCP was diagnosed in 12 healthy donors. In EDTA samples, the average number of platelets was 66.8 × 109/l (SD ± 30.8 × 109/l ) and 209.9 × 109/l (SD ± 46.6 x 109/l) in citrate samples. EDTA-dependant IgG and IgM class antibodies were found in the sera of 10 donors. In 2 donors, PTCP diagnosis was based only on the differences between platelet counts in EDTA and in citrate samples.
Conclusion: The PTCP frequency in blood donors was calculated at 1:6957, that is 0.014%.
Get Citation

Keywords

pseudothrombocytopenia; EDTA-dependent anti-platelet antibodies; blood donors

About this article
Title

The frequency of pseudothrombocytopenia in blood donors from the central region of Poland

Journal

Journal of Transfusion Medicine

Issue

Vol 1, No 1 (2008)

Pages

36-39

Published online

2009-02-02

Bibliographic record

Journal of Transfusion Medicine 2008;1(1):36-39.

Keywords

pseudothrombocytopenia
EDTA-dependent anti-platelet antibodies
blood donors

Authors

Dioniza Marciniak-Bielak
Bolesława Stróżowska
Anna Główka
Andrzej Szczepiński
Krystyna Maślanka

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