open access

Vol 2, No 1 (2009)
Research paper
Published online: 2009-02-12
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HCV RNA and HIV RNA detection by Procleix HIV-1/HCV Assay in blood donors with various results of anti-HCV and anti-HIV EIA

Piotr Grabarczyk, Joanna Medyńska, Grzegorz Liszewski, Dorota Kubicka-Russel, Ewa Sulkowska, Maria Mikulska, Magdalena Łętowska, Ewa Brojer
Journal of Transfusion Medicine 2009;2(1):26-33.

open access

Vol 2, No 1 (2009)
ORIGINAL PAPERS
Published online: 2009-02-12

Abstract


Background: The aim of the study was to investigate the sensitivity of Procleix HIV1/HCV Assay for HIV RNA and HCV RNA detection, and to analyse the frequency of viral nucleic acid detection in blood donors with various EIA test results.
Material and methods: The 95% sensitivity of the Procleix test was 6.2 IU HCV RNA /ml and 44.5 IU HIV RNA /ml. HCV RNA was detected in 71/392 (18.1%) anti-HCV positive and HIV RNA in 21/557 (3.8%) EIA HIV-reactive donors. The frequency of HCV RNA detection correlated with signal/cut-off ratios (S/C ratio) of EIA. HCV RNA was found in 68/105 (64.8%) of the donors if it was >4, in 1/85 (1.2%) if it was between 2.00 and 3.99, and in 2 out of 202 donors (1%) if it was between 1.00 and 1.99. HIV RNA was detected in all 21 blood donors positive in EIA and Western Blot.
Results and conclusions: The study demonstrated that Procleix HIV1/HCV Assay is very sensitive and can be used in the reference laboratory to confirm active infection in donors with positive results. We observed a low frequency of active infection in Polish blood donors with repeated reactive results in HCV and HIV EIA. The high S/C ratio value (> 4) of EIA is a good predictor of HCV RNA detection, but HCV RNA can also be detected in single donors with low ratio values of the EIA test.

Abstract


Background: The aim of the study was to investigate the sensitivity of Procleix HIV1/HCV Assay for HIV RNA and HCV RNA detection, and to analyse the frequency of viral nucleic acid detection in blood donors with various EIA test results.
Material and methods: The 95% sensitivity of the Procleix test was 6.2 IU HCV RNA /ml and 44.5 IU HIV RNA /ml. HCV RNA was detected in 71/392 (18.1%) anti-HCV positive and HIV RNA in 21/557 (3.8%) EIA HIV-reactive donors. The frequency of HCV RNA detection correlated with signal/cut-off ratios (S/C ratio) of EIA. HCV RNA was found in 68/105 (64.8%) of the donors if it was >4, in 1/85 (1.2%) if it was between 2.00 and 3.99, and in 2 out of 202 donors (1%) if it was between 1.00 and 1.99. HIV RNA was detected in all 21 blood donors positive in EIA and Western Blot.
Results and conclusions: The study demonstrated that Procleix HIV1/HCV Assay is very sensitive and can be used in the reference laboratory to confirm active infection in donors with positive results. We observed a low frequency of active infection in Polish blood donors with repeated reactive results in HCV and HIV EIA. The high S/C ratio value (> 4) of EIA is a good predictor of HCV RNA detection, but HCV RNA can also be detected in single donors with low ratio values of the EIA test.
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Keywords

Anti-HCV; anti-HIV; HIV RNA; HCV RNA; transcription-mediated amplification

About this article
Title

HCV RNA and HIV RNA detection by Procleix HIV-1/HCV Assay in blood donors with various results of anti-HCV and anti-HIV EIA

Journal

Journal of Transfusion Medicine

Issue

Vol 2, No 1 (2009)

Article type

Research paper

Pages

26-33

Published online

2009-02-12

Bibliographic record

Journal of Transfusion Medicine 2009;2(1):26-33.

Keywords

Anti-HCV
anti-HIV
HIV RNA
HCV RNA
transcription-mediated amplification

Authors

Piotr Grabarczyk
Joanna Medyńska
Grzegorz Liszewski
Dorota Kubicka-Russel
Ewa Sulkowska
Maria Mikulska
Magdalena Łętowska
Ewa Brojer

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