open access
Leukodepleted red blood cell concentrates frozen and thawed in ACP 215 system; evaluation study
open access
Abstract
Material and methods: In the study we used 30 leuko-depleted RBC units. The following parameters were determined: Hb concentration, Ht, haemolysis, pH, K+ concentration, glucose concentration, LDH activity and osmotic resistance of red blood cells. The study was performed during 7-day storage of leuko-depleted RBCs after thawing.
Results: In the first study-stage (freezing and thawing of leuko-depleted RBCs after RBC filtration) the Ht and Hb values were significantly higher in the leuko-depleted RBCs control group than in the study group. In the second study-stage (freezing and thawing of leukodepleted RBCs obtained after WB filtration) the parameters were within normal quality control range.
Conclusions: How RBCs are prepared for glycerolization has significant impact on the quality of the component after thawing, the Military Blood Transfusion Center is therefore planning to implement the method of freezing leuko-depleted RBCs obtained in the procedure of in-line filtration.
J. Transf. Med. 2011; 1: 32–44
Abstract
Material and methods: In the study we used 30 leuko-depleted RBC units. The following parameters were determined: Hb concentration, Ht, haemolysis, pH, K+ concentration, glucose concentration, LDH activity and osmotic resistance of red blood cells. The study was performed during 7-day storage of leuko-depleted RBCs after thawing.
Results: In the first study-stage (freezing and thawing of leuko-depleted RBCs after RBC filtration) the Ht and Hb values were significantly higher in the leuko-depleted RBCs control group than in the study group. In the second study-stage (freezing and thawing of leukodepleted RBCs obtained after WB filtration) the parameters were within normal quality control range.
Conclusions: How RBCs are prepared for glycerolization has significant impact on the quality of the component after thawing, the Military Blood Transfusion Center is therefore planning to implement the method of freezing leuko-depleted RBCs obtained in the procedure of in-line filtration.
J. Transf. Med. 2011; 1: 32–44
Keywords
red blood cell concentrate; cryopreservation; glycerolization; deglicerolization; leuko-depleted RBCs


Title
Leukodepleted red blood cell concentrates frozen and thawed in ACP 215 system; evaluation study
Journal
Journal of Transfusion Medicine
Issue
Article type
Research paper
Pages
32-43
Published online
2011-04-20
Bibliographic record
Journal of Transfusion Medicine 2011;4(1):32-43.
Keywords
red blood cell concentrate
cryopreservation
glycerolization
deglicerolization
leuko-depleted RBCs
Authors
Agnieszka Żak
Elżbieta Lachert
Jolanta Antoniewicz-Papis
Anna Tomaszewska
Wiesław Tomaszewski
Barbara Biedrzycka
Ewa Potocka
Edyta Jarmocik
Justyna Mazur
Dariusz Blinowski
Adam Olszewski