open access
Reticulated platelets — hematological norm and fluctuation in the number of reticulated platelets with time
open access
Abstract
Material and methods: Blood samples were freshly collected on EDTA anticoagulant from 148 healthy individuals. To determine the percentage/absolute RP values we used immunofluorescent double labelling with monoclonal antibody anti-CD41-phycoerythrin and thiazole orange. The RP level were analysed using flow cytometry.
Results: The haematological normal value is — 1.3 ± 0.8%, 3.29 ± 2.02 × 103/μl (absolute value); range — 0.5–6%, 1.26–15.2 x 103/μl (absolute number). For RP labelling it is recommended to use freshly collected blood samples. Reticulated platelets level in healthy individuals was stable and did not change significantly with time.
Conclusions: The hematological norms of RP established during our study are similar to the norms reported in literature.
J. Transf. Med. 2011; 4: 123–128
Abstract
Material and methods: Blood samples were freshly collected on EDTA anticoagulant from 148 healthy individuals. To determine the percentage/absolute RP values we used immunofluorescent double labelling with monoclonal antibody anti-CD41-phycoerythrin and thiazole orange. The RP level were analysed using flow cytometry.
Results: The haematological normal value is — 1.3 ± 0.8%, 3.29 ± 2.02 × 103/μl (absolute value); range — 0.5–6%, 1.26–15.2 x 103/μl (absolute number). For RP labelling it is recommended to use freshly collected blood samples. Reticulated platelets level in healthy individuals was stable and did not change significantly with time.
Conclusions: The hematological norms of RP established during our study are similar to the norms reported in literature.
J. Transf. Med. 2011; 4: 123–128
Keywords
reticulated platelets; RP hematological normal level; method for reticulated platelet evaluation


Title
Reticulated platelets — hematological norm and fluctuation in the number of reticulated platelets with time
Journal
Journal of Transfusion Medicine
Issue
Article type
Research paper
Pages
123-128
Published online
2011-09-23
Bibliographic record
Journal of Transfusion Medicine 2011;4(3):123-128.
Keywords
reticulated platelets
RP hematological normal level
method for reticulated platelet evaluation
Authors
Justyna Karandys
Dorota Siwik
Krystyna Maślanka