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Different serotypes of dengue virus (DENV) imported by Polish travellers from dengue endemic areas to Poland
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Abstract
Background: Dengue viruses are the most widespread arboviruses (transmitted mainly by Aedes aegypti and Ae. albopictus mosquitoes), which have shown an unexpected geographic expansion. There are four dengue virus serotypes: DENV-1, DENV-2, DENV-3, and DENV-4. Subsequent infections increase the risk of developing severe dengue fever. Materials and methods: Samples from travellers returning from the endemic area of dengue fever were examined with the reverse transcription-polymerase chain reaction method. Primers amplified 743 bp fragment of the NS5 and 3’UTR genomic region of mosquito-borne flaviviruses of JEV group were used. Results: The sequences from the 10 examined patients were compared to available DENV sequences in GenBank database and the basic local alignment search tool (BLAST) results confirmed that the infective virus was DENV-1 (6 patients), DENV-2 (2 patients) and DENV-3 (2 patients). Conclusions: For the first time in Poland, dengue virus serotypes were determined in travellers returning from dengue-endemic areas.
Abstract
Background: Dengue viruses are the most widespread arboviruses (transmitted mainly by Aedes aegypti and Ae. albopictus mosquitoes), which have shown an unexpected geographic expansion. There are four dengue virus serotypes: DENV-1, DENV-2, DENV-3, and DENV-4. Subsequent infections increase the risk of developing severe dengue fever. Materials and methods: Samples from travellers returning from the endemic area of dengue fever were examined with the reverse transcription-polymerase chain reaction method. Primers amplified 743 bp fragment of the NS5 and 3’UTR genomic region of mosquito-borne flaviviruses of JEV group were used. Results: The sequences from the 10 examined patients were compared to available DENV sequences in GenBank database and the basic local alignment search tool (BLAST) results confirmed that the infective virus was DENV-1 (6 patients), DENV-2 (2 patients) and DENV-3 (2 patients). Conclusions: For the first time in Poland, dengue virus serotypes were determined in travellers returning from dengue-endemic areas.
Keywords
dengue, DENV, dengue serotypes, travellers, Poland, reverse transcription-PCR
About this articleTitle
Different serotypes of dengue virus (DENV) imported by Polish travellers from dengue endemic areas to Poland
Journal
Issue
Article type
Original article
Pages
72-76
Published online
2015-06-17
Page views
1634
Article views/downloads
2508
DOI
10.5603/IMH.2015.0018
Pubmed
Bibliographic record
IMH 2015;66(2):72-76.
Keywords
dengue
DENV
dengue serotypes
travellers
Poland
reverse transcription-PCR
Authors
Beata Biernat
Joanna Stańczak
Beata Szostakowska
Agnieszka Wroczyńska
Anna Kuna
Wacław L. Nahorski
Maria Racewicz
