open access

Vol 66, No 2 (2015)
TROPICAL MEDICINE Original article
Published online: 2015-06-17
Submitted: 2015-06-17
Accepted: 2015-06-17
Get Citation

Different serotypes of dengue virus (DENV) imported by Polish travellers from dengue endemic areas to Poland

Beata Biernat, Joanna Stańczak, Beata Szostakowska, Agnieszka Wroczyńska, Anna Kuna, Wacław L. Nahorski, Maria Racewicz
DOI: 10.5603/IMH.2015.0018
·
Pubmed: 26119675
·
International Maritime Health 2015;66(2):72-76.

open access

Vol 66, No 2 (2015)
TROPICAL MEDICINE Original article
Published online: 2015-06-17
Submitted: 2015-06-17
Accepted: 2015-06-17

Abstract

Background: Dengue viruses are the most widespread arboviruses (transmitted mainly by Aedes aegypti and Ae. albopictus mosquitoes), which have shown an unexpected geographic expansion. There are four dengue virus serotypes: DENV-1, DENV-2, DENV-3, and DENV-4. Subsequent infections increase the risk of developing severe dengue fever. Materials and methods: Samples from travellers returning from the endemic area of dengue fever were examined with the reverse transcription-polymerase chain reaction method. Primers amplified 743 bp fragment of the NS5 and 3’UTR genomic region of mosquito-borne flaviviruses of JEV group were used. Results: The sequences from the 10 examined patients were compared to available DENV sequences in GenBank database and the basic local alignment search tool (BLAST) results confirmed that the infective virus was DENV-1 (6 patients), DENV-2 (2 patients) and DENV-3 (2 patients). Conclusions: For the first time in Poland, dengue virus serotypes were determined in travellers returning from dengue-endemic areas.

Abstract

Background: Dengue viruses are the most widespread arboviruses (transmitted mainly by Aedes aegypti and Ae. albopictus mosquitoes), which have shown an unexpected geographic expansion. There are four dengue virus serotypes: DENV-1, DENV-2, DENV-3, and DENV-4. Subsequent infections increase the risk of developing severe dengue fever. Materials and methods: Samples from travellers returning from the endemic area of dengue fever were examined with the reverse transcription-polymerase chain reaction method. Primers amplified 743 bp fragment of the NS5 and 3’UTR genomic region of mosquito-borne flaviviruses of JEV group were used. Results: The sequences from the 10 examined patients were compared to available DENV sequences in GenBank database and the basic local alignment search tool (BLAST) results confirmed that the infective virus was DENV-1 (6 patients), DENV-2 (2 patients) and DENV-3 (2 patients). Conclusions: For the first time in Poland, dengue virus serotypes were determined in travellers returning from dengue-endemic areas.

Get Citation

Keywords

dengue, DENV, dengue serotypes, travellers, Poland, reverse transcription-PCR

About this article
Title

Different serotypes of dengue virus (DENV) imported by Polish travellers from dengue endemic areas to Poland

Journal

International Maritime Health

Issue

Vol 66, No 2 (2015)

Pages

72-76

Published online

2015-06-17

DOI

10.5603/IMH.2015.0018

Pubmed

26119675

Bibliographic record

International Maritime Health 2015;66(2):72-76.

Keywords

dengue
DENV
dengue serotypes
travellers
Poland
reverse transcription-PCR

Authors

Beata Biernat
Joanna Stańczak
Beata Szostakowska
Agnieszka Wroczyńska
Anna Kuna
Wacław L. Nahorski
Maria Racewicz

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