Online first
Research paper
Published online: 2023-07-05

open access

Page views 372
Article views/downloads 253
Get Citation

Connect on Social Media

Connect on Social Media

Evaluation of serum levels of soluble (s)L- and (s)P-selectins in endometrial cancer

Dominika Majchrzak-Baczmanska12, Krzysztof Pogoda2, Anna Oblekowska2, Dariusz Owczarek2, Beata Antosiak1, Michal Wojciechowski12, Agnieszka Wosiak3, Andrzej Malinowski12

Abstract

Objectives: A number of reports on the role of selectin in the process of carcinogenesis, at the stage of proliferation and metastasis, have been available.

The aim of the study was to analyze (s)P- and (s)L-selectin serum concentrations in women with EC and to compare these concentrations to clinical/pathological parameters and disease progression using surgical-pathological staging data.

Material and methods: A total of 46 patients with EC and 50 healthy controls were included in the study. Serum concentrations of sL- and sP-selectins were measured in all participants. The oncologic protocol was implemented in all women from the study group.

Results: Significantly higher serum concentrations were found in EC women as compared to controls. No statistically significant differences were found between the concentrations of the soluble forms of selectins and the following parameters: histologic type of EC, histologic tumor differentiation, depth of myometrial infiltration, cervical involvement, distant metastases, vascular space invasion, and disease advancement. Slightly higher (s)P-selectin concentrations were observed in serous carcinoma, in women with cervical involvement, in the sera of women with vascular space invasion and with advanced stages of the disease. Slightly higher mean (s)P-selectin concentrations correlated with lower differentiation of the tumor. Slightly higher mean (s)P-selectin concentration was detected in the sera of women with lymph node metastases and with the serosal and/or adnexal involvement. The results were statistically insignificant, but they almost reached statistical significance.

Conclusions: L- and P-selectins play a role in the biology of EC. The absence of an unambiguous relationship between differences in (s)L- and (s)P-selectin levels and disease advancement suggests that they do not play a vital role in tumor progression in endometrial cancer.

Article available in PDF format

View PDF Download PDF file

References

  1. Majchrzak-Baczmańska DB, Głowacka E, Wilczyński M, et al. Serum concentrations of soluble (s)L- and (s)P-selectins in women with ovarian cancer. Prz Menopauzalny. 2018; 17(1): 11–17.
  2. Southcott BM. Carcinoma of the endometrium. Drugs. 2001; 61(10): 1395–1405.
  3. Mantur M, Wojszel J. Cząsteczki adhezyjne oraz ich udział w procesie zapalnym i nowotworowym. Pol Merk Lek. 2008; XXIV(1140): 177.
  4. Czygier M, Ławicki S, Gacuta-Szumarska E, et al. Stężenie sL-selektyny i mieloperoksydazy (MPO) oraz czynnika wzrostu kolonii granulocytarnych (G-CSF) w osoczu pacjentek z nowotworami macicy. Przegl Lek. 2010; 67(3): 184–186.
  5. Dymicka-Piekarska V, Matowicka-Karna J, Gryko M, et al. Relationship between soluble P-selectin and inflammatory factors (interleukin-6 and C-reactive protein) in colorectal cancer. Thromb Res. 2007; 120(4): 585–590.
  6. Egan K, Crowley D, Smyth P, et al. Platelet adhesion and degranulation induce pro-survival and pro-angiogenic signalling in ovarian cancer cells. PLoS One. 2011; 6(10): e26125.
  7. Bazou D, Santos-Martinez MJ, Medina C, et al. Elucidation of flow-mediated tumour cell-induced platelet aggregation using an ultrasound standing wave trap. Br J Pharmacol. 2011; 162(7): 1577–1589.
  8. Garcia J, Callewaert N, Borsig L. P-selectin mediates metastatic progression through binding to sulfatides on tumor cells. Glycobiology. 2007; 17(2): 185–196.
  9. Suzuki Y, Toda Y, Tamatani T, et al. Sulfated glycolipids are ligands for a lymphocyte homing receptor, L-selectin (LECAM-1), Binding epitope in sulfated sugar chain. Biochem Biophys Res Commun. 1993; 190(2): 426–434.
  10. Sugiyama T, Miyazawa M, Mikami M, et al. Enhanced expression of sulfatide, a sulfated glycolipid, in well-differentiated endometrial adenocarcinoma. Int J Gynecol Cancer. 2012; 22(7): 1192–1197.
  11. Lim SC, Oh SH. The role of CD24 in various human epithelial neoplasias. Pathol Res Pract. 2005; 201(7): 479–486.
  12. Aigner S, Sthoeger ZM, Fogel M, et al. CD24, a mucin-type glycoprotein, is a ligand for P-selectin on human tumor cells. Blood. 1997; 89(9): 3385–3395.
  13. Ferroni P, Roselli M, Martini F, et al. Prognostic value of soluble P-selectin levels in colorectal cancer. Int J Cancer. 2004; 111(3): 404–408.
  14. Dymicka-Piekarska V, Kemona H. Does colorectal cancer clinical advancement affect adhesion molecules (sP-selectin, sE-selectin and ICAM-1) concentration? Thromb Res. 2009; 124(1): 80–83.
  15. Haznedaroglu IC, Benekli M, Ozcebe O, et al. Serum L-selectin and P-selectin levels in lymphomas. Haematologia (Budap). 2000; 30(1): 27–30.
  16. Aki SZ, Sucak GT, Paşaoğlu H, et al. Thrombopoietic cytokine and P-selectin levels in patients with multiple myeloma undergoing autologous stem cell transplantation: decrease in posttransplantation P-selectin levels might predict the degree of maximum response. Clin Lymphoma Myeloma. 2009; 9(3): 229–233.
  17. Roselli M, Mineo TC, Martini F, et al. Soluble selectin levels in patients with lung cancer. Int J Biol Markers. 2002; 17(1): 56–62.
  18. Schadendorf D, Diehl S, Zuberbier T, et al. Quantitative detection of soluble adhesion molecules in sera of melanoma patients correlates with clinical stage. Dermatology. 1996; 192(2): 89–93.
  19. Czygier M, Ławicki S, Szmitkowski M. The plasma level of sL-selectin, myeloperoxidase (MPO) and granulocyte-colony stimulating factor (G-CSF) in breast cancer patients after surgery. Przegl Lek. 2009; 66(8): 433–436.
  20. Chang JH, Qi ZH, Chen FP, et al. [Clinical significance of the detection of the sL-selectin level in patients with acute leukemia]. Hunan Yi Ke Da Xue Xue Bao. 2002; 27(2): 151–153.
  21. Izycka A, Jabłońska E, Izycki T, et al. Expression of L-selectin on the surface of neutrophils stimulated by TNF-alpha and level of sL-selectin in serum of patients with lung cancer. Pol Merkur Lekarski. 2005; 18(103): 62–65.
  22. Spertini O, Callegari P, Cordey AS, et al. High levels of the shed form of L-selectin are present in patients with acute leukemia and inhibit blast cell adhesion to activated endothelium. Blood. 1994; 84(4): 1249–1256.
  23. Kiersnowska-Rogowska B, Izycka A, Jabłońska E, et al. Estimation of L-selectin expression on neutrophils and level of soluble L-selectin form in serum of patient with chronic myelogenic leukemia. Przegl Lek. 2006; 63(9): 756–758.
  24. Aref S, Salama O, Al-Tonbary Y, et al. L and E selectins in acute myeloid leukemia: expression, clinical relevance and relation to patient outcome. Hematology. 2002; 7(2): 83–87.
  25. Olejnik I. Serum soluble L-selectin in childhood acute lymphoblastic leukemia. Pediatr Int. 1999; 41(3): 246–248.
  26. Czygier M, Ławicki S, Uścinowicz A, et al. The plasma level of sL-selectin, myeloperoxidase and granulocyte-colony stimulating factor (G-CSF) in breast cancer patients in the course of chemotherapy. Przegl Lek. 2008; 65(3): 115–118.
  27. Läubli H, Spanaus KS, Borsig L. Selectin-mediated activation of endothelial cells induces expression of CCL5 and promotes metastasis through recruitment of monocytes. Blood. 2009; 114(20): 4583–4591.
  28. Läubli H, Stevenson JL, Varki A, et al. L-selectin facilitation of metastasis involves temporal induction of Fut7-dependent ligands at sites of tumor cell arrest. Cancer Res. 2006; 66(3): 1536–1542.
  29. Chen Z, Jing Y, Song B, et al. Chemically modified heparin inhibits in vitro L-selectin-mediated human ovarian carcinoma cell adhesion. Int J Gynecol Cancer. 2009; 19(4): 540–546.