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Clinical use of redox biomarkers for diagnosis of male infertility

Marek Szymanski123, Radoslaw Janicki23, Piotr Kaminski4, Karolina Wasilow3, Jaromir Soltysiak5, Robert Szyca6, Angelika Szymanska7, Marcin Arabik8
DOI: 10.5603/gpl.98046
·
Pubmed: 38334352
Affiliations
  1. Department of Obstetrics and Gynecology, Ludwik Rydygier Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Torun, Poland, Bydgoszcz
  2. Gynecology, Oncological Gynecology and Gynecological Endocrynology Clinic, 10th Military Research Hospital and Polyclinic, IPHC, Bydgoszcz, Poland
  3. NZOZ Medical Center, Clinic of Infertility Treatment “Genesis”, Bydgoszcz, Poland
  4. Department of Ecology and Environmental Protection, Department of Medical Biology and Biochemistry, Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Toruń, Bydgoszcz, Poland
  5. “Solmed Medical” Center Chojnice, Poland
  6. Clinic of Surgery and Oncological Surgery, 10th Military Research Hospital and Polyclinic, IPHC, Bydgoszcz, Poland.
  7. The National Institute of Medicine of the Ministry of Interior and Administration, Warsaw, Poland
  8. Gynecology, Obstetrics and Pregnancy Pathology Department, Multi-speciality City Hospital in Bydgoszcz, Poland

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Ahead of Print
ORIGINAL PAPERS Gynecology
Published online: 2024-01-05

Abstract

The aim of the study was to analyze the activity of antioxidant enzymes (glutathione reductase, catalase, superoxide dismutase) and malondialdehyde (MDA) levels in a population of men with abnormal semen parameters and in a population of men diagnosed with normozoospermia. This study was performed using data collected at the Infertility Treatment Clinic ‚Genesis’ , Bydgoszcz, Poland, between 1 January 2011 and 31 December 2017. A total number of 321 men meeting the inclusion criteria were selected and divided into the control group (162 men) and the infertility group (159 men). The activities of superoxide dismutase (SOD), catalase (CAT), and glutathione reductase (GR) were measured using ready-made kits; lipid peroxidation intensity was determined by the thiobarbituric acid method. No statistically significant differences were found for the activity of SOD, GR, CAT between the groups. MDA values measured in the serum of patients in the healthy group were higher than in the group with semenological disorders. Although our study did not demonstrate the usefulness of the above blood tests, further studies are needed to explore the potential use of assessing redox parameters to develop new diagnostic and therapeutic approaches for male infertility.

Abstract

The aim of the study was to analyze the activity of antioxidant enzymes (glutathione reductase, catalase, superoxide dismutase) and malondialdehyde (MDA) levels in a population of men with abnormal semen parameters and in a population of men diagnosed with normozoospermia. This study was performed using data collected at the Infertility Treatment Clinic ‚Genesis’ , Bydgoszcz, Poland, between 1 January 2011 and 31 December 2017. A total number of 321 men meeting the inclusion criteria were selected and divided into the control group (162 men) and the infertility group (159 men). The activities of superoxide dismutase (SOD), catalase (CAT), and glutathione reductase (GR) were measured using ready-made kits; lipid peroxidation intensity was determined by the thiobarbituric acid method. No statistically significant differences were found for the activity of SOD, GR, CAT between the groups. MDA values measured in the serum of patients in the healthy group were higher than in the group with semenological disorders. Although our study did not demonstrate the usefulness of the above blood tests, further studies are needed to explore the potential use of assessing redox parameters to develop new diagnostic and therapeutic approaches for male infertility.

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Keywords

male infertility; oxidative stress; reactive oxygen species; superoxide dismutase; catalase; glutathione reductase; MDA; antioxidant defense

About this article
Title

Clinical use of redox biomarkers for diagnosis of male infertility

Journal

Ginekologia Polska

Issue

Ahead of Print

Article type

Research paper

Published online

2024-01-05

Page views

232

Article views/downloads

151

DOI

10.5603/gpl.98046

Pubmed

38334352

Keywords

male infertility
oxidative stress
reactive oxygen species
superoxide dismutase
catalase
glutathione reductase
MDA
antioxidant defense

Authors

Marek Szymanski
Radoslaw Janicki
Piotr Kaminski
Karolina Wasilow
Jaromir Soltysiak
Robert Szyca
Angelika Szymanska
Marcin Arabik

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