Objective: The objective of the study was to analyze a possible association between cognitive functions and level of prolactin in menopausal women with different polymorphisms of apolipoprotein E gene (APOE). The examined population included women from the south-eastern part of Poland; aged 50-65 years; at least 2 years after their last menstruation; in good health; with at least primary education, FSH>30 mlU/ml. The MoCA test (Montreal Cognitive Assessment Test) allowed us to exclude women with signs of dementia. The cognitive functions assessment was conducted with the CNS-VitalSigns diagnostic equipment (Polish version). The prolactin designations were conducted by SYNEVO – an accredited laboratory. The examination of APOE polymorphism was performed using the multiplex-PCR method. The results were statistically analyzed. Results and conclusion: Higher level of prolactin turned out to be associated with better test results in the following areas: NCI, memory, verbal memory, psychomotor speed and concentration. Women with higher level of prolactin had better results in NCI, psychomotor speed and verbal memory tests. The test results of other cognitive function were not unequivocally related to higher levels of prolactin. Thus, it was not possible to conclude that the presence of APOE polymorphism is related to the effect of prolactin on cognitive functions of the examined menopausal women.