Role of MMP-8 in photoaging and photocarcinogenesis
Abstract
Introduction: Ultraviolet radiation (UVR) is especially harmful to human skin. It mostly contributes to skin photoaging that is associated with skin carcinomas, such as the most common skin cancer — basal cell carcinoma (BCC). It has been shown that significant role in skin carcinogenesis plays short-wavelength UVB radiation. What is more, UVR by changing the expression of matrix metalloproteinases (MMPs) in skin contributes to photoaging and plays an important role in photocarcinogenesis. MMP-8 is one of collagenases that is released during inflammatory process.
Material and methods: The study group consists of 85 individuals (22 patients with diagnosed BCC and 63 healthy volunteers that have been exposed to various doses of UVB radiation) in order to evaluate the expression of MMP-8 protein in skin biopsies. The Western-blot method was used to analyse the results.
Results: The expression of MMP-8 has been observed in all skin biopsies. The performed analysis did not present statistical significant difference in expression of MMP-8 between skin samples with BCC or exposed to UVR in comparison to healthy skin samples.
Conclusions: The role of MMP-8 in skin photoaging has little activity in skin following UVB radiation and it is slightly
probable that MMP-8 contributes to photoaging and photocancerogenesis.
Keywords: ultra violet radiationphotocancerogenesismatrix metalloproteinases-8
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- Gilchrest BA, Yaar M. Ageing and photoageing of the skin: observations at the cellular and molecular level. Br J Dermatol. 1992; 127 Suppl 41: 25–30.
- Kaminer MS. Photodamage: magnitude of the problem. Gilchrest BA, editor. Cambridge: Blackwell Science,; Photodamage. 1995:pp 1–11.
- Tang JY, So PL, Epstein EH. Novel Hedgehog pathway targets against basal cell carcinoma. Toxicol Appl Pharmacol. 2007; 224(3): 257–264.
- Lupi O. Correlations between the Sonic Hedgehog pathway and basal cell carcinoma. Int J Dermatol. 2007; 46(11): 1113–1117.
- Talwar HS, Griffiths CE, Fisher GJ, et al. Reduced type I and type III procollagens in photodamaged adult human skin. J Invest Dermatol. 1995; 105(2): 285–290.
- McCawley LJ, Matrisian LM. Matrix metalloproteinases: they're not just for matrix anymore! Curr Opin Cell Biol. 2001; 13(5): 534–540.
- Quan T, Qin Z, Xia W, et al. Matrix-degrading metalloproteinases in photoaging. J Investig Dermatol Symp Proc. 2009; 14(1): 20–24.
- Kim J, Lee CW, Kim EK, et al. Inhibition effect of Gynura procumbens extract on UV-B-induced matrix-metalloproteinase expression in human dermal fibroblasts. J Ethnopharmacol. 2011; 137(1): 427–433.
- O'Grady A, Dunne C, O'Kelly P, et al. Differential expression of matrix metalloproteinase (MMP)-2, MMP-9 and tissue inhibitor of metalloproteinase (TIMP)-1 and TIMP-2 in non-melanoma skin cancer: implications for tumour progression. Histopathology. 2007; 51(6): 793–804.
- Sbardella D, Fasciglione GF, Gioia M, et al. Human matrix metalloproteinases: an ubiquitarian class of enzymes involved in several pathological processes. Mol Aspects Med. 2012; 33(2): 119–208.
- Van Lint P, Libert C. Matrix metalloproteinase-8: cleavage can be decisive. Cytokine Growth Factor Rev. 2006; 17(4): 217–223.
- Hasty KA, Pourmotabbed TF, Goldberg GI, et al. Human neutrophil collagenase. A distinct gene product with homology to other matrix metalloproteinases. J Biol Chem. 1990; 265(20): 11421–11424.
- Hasty KA, Pourmotabbed TF, Goldberg GI, et al. Human neutrophil collagenase. A distinct gene product with homology to other matrix metalloproteinases. J Biol Chem. 1990; 265(20): 11421–11424.
- Fisher GJ, Choi HC, Bata-Csorgo Z, et al. Ultraviolet irradiation increases matrix metalloproteinase-8 protein in human skin in vivo. J Invest Dermatol. 2001; 117(2): 219–226.
- Moro N, Mauch C, Zigrino P. Metalloproteinases in melanoma. Eur J Cell Biol. 2014; 93(1-2): 23–29.
- Brennan M, Bhatti H, Nerusu KC, et al. Matrix metalloproteinase-1 is the major collagenolytic enzyme responsible for collagen damage in UV-irradiated human skin. Photochem Photobiol. 2003; 78(1): 43–48.
- Fisher GJ, Wang ZQ, Datta SC, et al. Pathophysiology of premature skin aging induced by ultraviolet light. N Engl J Med. 1997; 337(20): 1419–1428.
- Sbardella D, Fasciglione GF, Gioia M, et al. Human matrix metalloproteinases: an ubiquitarian class of enzymes involved in several pathological processes. Mol Aspects Med. 2012; 33(2): 119–208.
