open access

Vol 82, No 3 (2023)
Original article
Submitted: 2022-06-10
Accepted: 2022-06-30
Published online: 2022-07-08
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Curcumin reduces blood-nerve barrier abnormalities and cytotoxicity to endothelial cells and pericytes induced by cisplatin

P. Kobutree1, A. Tothonglor1, A. Roumwong1, D. Jindatip1, S. Agthong1
·
Pubmed: 35818807
·
Folia Morphol 2023;82(3):533-542.
Affiliations
  1. Department of Anatomy, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand

open access

Vol 82, No 3 (2023)
ORIGINAL ARTICLES
Submitted: 2022-06-10
Accepted: 2022-06-30
Published online: 2022-07-08

Abstract

Background: Cisplatin is a platinum-based antineoplastic agent used to treat
cancers of solid organs. Neuropathy is one of its major side effects, necessitating
dose reduction or cessation. Previous studies suggested that cisplatin causes
microvascular toxicity, including pericyte detachment. This study aimed to clarify
whether these alterations occurred in the blood–nerve barrier (BNB) of capillaries
after cisplatin treatment.
Materials and methods and Results: Electron microscopic analysis of rat sciatic
nerves with cisplatin neuropathy showed increased frequency and severity of
pericyte detachment. Moreover, the vascular basement membrane did not tightly
encircle around the endothelial cells and pericytes. Cultured human umbilical vein
endothelial cells and human brain vascular pericytes showed reduced viability,
increased caspase-3 activity and enhanced oxidative stress following cisplatin
treatment. In addition, cisplatin decreased transendothelial electrical resistance
(TEER) and the expression of the tight junction proteins occludin and zonula
occludens-1. Curcumin, a polyphenol found in the root of Curcuma longa, had
favourable effects on cisplatin neuropathy in previous work. Therefore, curcumin
was tested to determine whether it had any effect on these abnormalities. Curcumin
alleviated pericyte detachment, cytotoxicity, oxidative stress, TEER reduction
and tight junction protein expression.
Conclusions: These data indicate that cisplatin causes BNB disruption in the
nerves and might result in neuropathy. Curcumin might improve neuropathy via
the restoration of BNB. Whether alterations in the BNB occur and curcumin is
effective in patients with cisplatin neuropathy remain to be investigated.

Abstract

Background: Cisplatin is a platinum-based antineoplastic agent used to treat
cancers of solid organs. Neuropathy is one of its major side effects, necessitating
dose reduction or cessation. Previous studies suggested that cisplatin causes
microvascular toxicity, including pericyte detachment. This study aimed to clarify
whether these alterations occurred in the blood–nerve barrier (BNB) of capillaries
after cisplatin treatment.
Materials and methods and Results: Electron microscopic analysis of rat sciatic
nerves with cisplatin neuropathy showed increased frequency and severity of
pericyte detachment. Moreover, the vascular basement membrane did not tightly
encircle around the endothelial cells and pericytes. Cultured human umbilical vein
endothelial cells and human brain vascular pericytes showed reduced viability,
increased caspase-3 activity and enhanced oxidative stress following cisplatin
treatment. In addition, cisplatin decreased transendothelial electrical resistance
(TEER) and the expression of the tight junction proteins occludin and zonula
occludens-1. Curcumin, a polyphenol found in the root of Curcuma longa, had
favourable effects on cisplatin neuropathy in previous work. Therefore, curcumin
was tested to determine whether it had any effect on these abnormalities. Curcumin
alleviated pericyte detachment, cytotoxicity, oxidative stress, TEER reduction
and tight junction protein expression.
Conclusions: These data indicate that cisplatin causes BNB disruption in the
nerves and might result in neuropathy. Curcumin might improve neuropathy via
the restoration of BNB. Whether alterations in the BNB occur and curcumin is
effective in patients with cisplatin neuropathy remain to be investigated.

Get Citation

Keywords

capillaries, cisplatin, nerve, neuropathy

About this article
Title

Curcumin reduces blood-nerve barrier abnormalities and cytotoxicity to endothelial cells and pericytes induced by cisplatin

Journal

Folia Morphologica

Issue

Vol 82, No 3 (2023)

Article type

Original article

Pages

533-542

Published online

2022-07-08

Page views

1358

Article views/downloads

975

DOI

10.5603/FM.a2022.0065

Pubmed

35818807

Bibliographic record

Folia Morphol 2023;82(3):533-542.

Keywords

capillaries
cisplatin
nerve
neuropathy

Authors

P. Kobutree
A. Tothonglor
A. Roumwong
D. Jindatip
S. Agthong

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