open access
Potential therapeutic role of microvesicles derived from mesenchymal stem cells and platelet-rich plasma in murine burn wound healing: scar regulation and antioxidant mechanism
, 2
Affiliations- Department of Anatomy and Embryology, Faculty of Medicine, Cairo University, Egypt
- Anatomy and Embryology Department, Faculty of Medicine, Ain Shams University, Egypt
open access
Abstract
Background: Microvesicles (MVs) derived from mesenchymal stem cells exhibited
an emerging promising therapy in many animal model diseases. Post-burn scars
represent one of the significant challenges in wound healing processes. The present
study investigated the possible role of MVs derived from mesenchymal stem cells
vs. platelet-rich plasma (PRP) in murine burn wound healing.
Materials and methods: Wistar rats (n = 40) were assigned into four equal
groups (control, burn, burn + PRP, burn + MVs). Small-sized burns were induced,
morphologically followed for 3 weeks, then rats were sacrificed and skin lesions
were analysed biochemically and immunohistochemically.
Results: Both MVs and PRP modulated the burn healing process with better results
in the MVs group than in PRP. MVs significantly (p < 0.05) accelerated burn
wound size healing and dramatically modulated tissue interleukin (IL)-10, IL-6, and
hyaluronidase. Both MVs and PRP significantly downregulated gene expression of
miRNA203 and alpha smooth muscle actin and immunoblotting analysis of matrix
metalloproteinases 3 and transforming growth factor beta compared with the
burn group. The immune-staining intensity of tumour necrosis factor alpha was
dramatically reversed in the MVs group compared with the burn group, whereas
that of connective tissue growth factor, collagen I and III was significantly reduced
in both groups. The antioxidant Nrf2 immune-staining intensity had been dramatically
enhanced particularly in MVs.
Conclusions: Microvesicles derived from mesenchymal stem cells and PRP may
improve burn wound healing via regulating scar formation and antioxidant mechanism.
Abstract
Background: Microvesicles (MVs) derived from mesenchymal stem cells exhibited
an emerging promising therapy in many animal model diseases. Post-burn scars
represent one of the significant challenges in wound healing processes. The present
study investigated the possible role of MVs derived from mesenchymal stem cells
vs. platelet-rich plasma (PRP) in murine burn wound healing.
Materials and methods: Wistar rats (n = 40) were assigned into four equal
groups (control, burn, burn + PRP, burn + MVs). Small-sized burns were induced,
morphologically followed for 3 weeks, then rats were sacrificed and skin lesions
were analysed biochemically and immunohistochemically.
Results: Both MVs and PRP modulated the burn healing process with better results
in the MVs group than in PRP. MVs significantly (p < 0.05) accelerated burn
wound size healing and dramatically modulated tissue interleukin (IL)-10, IL-6, and
hyaluronidase. Both MVs and PRP significantly downregulated gene expression of
miRNA203 and alpha smooth muscle actin and immunoblotting analysis of matrix
metalloproteinases 3 and transforming growth factor beta compared with the
burn group. The immune-staining intensity of tumour necrosis factor alpha was
dramatically reversed in the MVs group compared with the burn group, whereas
that of connective tissue growth factor, collagen I and III was significantly reduced
in both groups. The antioxidant Nrf2 immune-staining intensity had been dramatically
enhanced particularly in MVs.
Conclusions: Microvesicles derived from mesenchymal stem cells and PRP may
improve burn wound healing via regulating scar formation and antioxidant mechanism.
Keywords
microvesicles, stem cells, platelet-rich plasma, burn, miRNA203, rats
About this articleTitle
Potential therapeutic role of microvesicles derived from mesenchymal stem cells and platelet-rich plasma in murine burn wound healing: scar regulation and antioxidant mechanism
Journal
Issue
Article type
Original article
Pages
656-667
Published online
2022-06-22
Page views
1260
Article views/downloads
900
DOI
Pubmed
Bibliographic record
Folia Morphol 2023;82(3):656-667.
Keywords
microvesicles
stem cells
platelet-rich plasma
burn
miRNA203
rats
Authors
R. A. Imam
M. M. Amer
References (23)- An Y, Lin S, Tan X, et al. Exosomes from adipose‐derived stem cells and application to skin wound healing. Cell Proliferation. 2021; 54(3).
- Baglio SR, Pegtel DM, Baldini N. Mesenchymal stem cell secreted vesicles provide novel opportunities in (stem) cell-free therapy. Front Physiol. 2012; 3: 359.
- Blazquez R, Sanchez-Margallo FM, de la Rosa O, et al. Immunomodulatory Potential of Human Adipose Mesenchymal Stem Cells Derived Exosomes on in vitro Stimulated T Cells. Front Immunol. 2014; 5: 556.
- Bruno S, Grange C, Deregibus MC, et al. Mesenchymal stem cell-derived microvesicles protect against acute tubular injury. J Am Soc Nephrol. 2009; 20(5): 1053–1067.
- Feng CJ, Lin CH, Tsai CH, et al. Adipose-derived stem cells-induced burn wound healing and regeneration of skin appendages in a novel skin island rat model. J Chin Med Assoc. 2019; 82(8): 635–642.
- Gatti S, Bruno S, Deregibus MC, et al. Microvesicles derived from human adult mesenchymal stem cells protect against ischaemia-reperfusion-induced acute and chronic kidney injury. Nephrol Dial Transplant. 2011; 26(5): 1474–1483.
- Hosni Ahmed H, Rashed LA, Mahfouz S, et al. Can mesenchymal stem cells pretreated with platelet-rich plasma modulate tissue remodeling in a rat with burned skin? Biochem Cell Biol. 2017; 95(5): 537–548.
- Hosseini Mansoub N, Gürdal M, Karadadaş E, et al. The role of PRP and adipose tissue-derived keratinocytes on burn wound healing in diabetic rats. Bioimpacts. 2018; 8(1): 5–12.
- Imam RA, Rizk AAE. Efficacy of erythropoietin-pretreated mesenchymal stem cells in murine burn wound healing: possible in vivo transdifferentiation into keratinocytes. Folia Morphol. 2019; 78(4): 798–808.
- Kranendonk MEG, Visseren FLJ, van Balkom BWM, et al. Human adipocyte extracellular vesicles in reciprocal signaling between adipocytes and macrophages. Obesity (Silver Spring). 2014; 22(5): 1296–1308.
- Kumar V, Abbas AB, Aster JC. Inflammation and Repair. Chapter 3. In: Kumar V, Abbas AB (eds.) Robbins Basic Pathology. 10th ed. Elsevier 2018: 57–96.
- Li X, Liu L, Yang J, et al. Exosome derived from human umbilical cord mesenchymal stem cell mediates MiR-181c attenuating burn-induced excessive inflammation. EBioMedicine. 2016; 8: 72–82.
- Li X, Xie X, Lian W, et al. Exosomes from adipose-derived stem cells overexpressing Nrf2 accelerate cutaneous wound healing by promoting vascularization in a diabetic foot ulcer rat model. Exp Mol Med. 2018; 50(4): 1–14.
- Marshall CD, Hu MS, Leavitt T, et al. Cutaneous scarring: basic science, current treatments, and future directions. Adv Wound Care (New Rochelle). 2018; 7(2): 29–45.
- Ren S, Chen J, Duscher D, et al. Microvesicles from human adipose stem cells promote wound healing by optimizing cellular functions via AKT and ERK signaling pathways. Stem Cell Res Ther. 2019; 10(1): 47.
- Sanderson S, Wild G, Cull AM. et al.. Immunohistochemical and immunofluorescent techniques. In: Suvarna SK, Layton C, Bancroft JD (eds.) Bancroft’s Theory and Practice of Histological Techniques. 8th Edition. Elsevier Limited 2019: 337–394.
- Vinish M, Cui W, Stafford E, et al. Dendritic cells modulate burn wound healing by enhancing early proliferation. Wound Repair Regen. 2016; 24(1): 6–13.
- Viticchiè G, Lena AM, Cianfarani F, et al. MicroRNA-203 contributes to skin re-epithelialization. Cell Death Dis. 2012; 3(11): e435.
- Walmsley GG, Maan ZN, Wong VW, et al. Scarless wound healing: chasing the holy grail. Plast Reconstr Surg. 2015; 135(3): 907–917.
- Wang Lu, Hu Li, Zhou X, et al. Exosomes secreted by human adipose mesenchymal stem cells promote scarless cutaneous repair by regulating extracellular matrix remodelling. Sci Rep. 2017; 7(1): 13321.
- Xiao Li, Lingying L, Jing Y, et al. Exosome derived from human umbilical cord mesenchymal stem cell mediates MiR-181c attenuating burn-induced excessive inflammation. EBioMedicine. 2016; 8: 72–82.
- Yan Y, Wu R, Bo Y, et al. Induced pluripotent stem cells-derived microvesicles accelerate deep second-degree burn wound healing in mice through miR-16-5p-mediated promotion of keratinocytes migration. Theranostics. 2020; 10(22): 9970–9983.
- Zaki SM, Algaleel WA, Imam RA, et al. Mesenchymal stem cells pretreated with platelet-rich plasma modulate doxorubicin-induced cardiotoxicity. Hum Exp Toxicol. 2019; 38(7): 857–874.
