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Vol 82, No 3 (2023)
Original article
Submitted: 2022-02-09
Accepted: 2022-04-07
Published online: 2022-04-28
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Protective effect of liriodendrin against liver ischaemia/reperfusion injury in mice via modulating oxidative stress, inflammation and nuclear factor kappa B/toll-like receptor 4 pathway

Z. Y. Yu1, G. Cheng234
DOI: 10.5603/FM.a2022.0049
·
Pubmed: 35607873
·
Folia Morphol 2023;82(3):668-676.
Affiliations
  1. Department of General Surgery, Panjin Central Hospital, Panjin, Liaoning Province, China
  2. Department of Intensive Care Unit, The People’s Hospital of Shimen County, Changde, Hunan Province, China
  3. Department of Clinical Medicine, Yiyang Medical College, Yiyang, Hunan Province, China
  4. Changsha Medical University, Changsha, Hunan Province, China

open access

Vol 82, No 3 (2023)
ORIGINAL ARTICLES
Submitted: 2022-02-09
Accepted: 2022-04-07
Published online: 2022-04-28

Abstract

Background: The aim of the present study was to investigate the protective effect
and mechanism of liriodendrin (LDN) is a lignan diglucoside in hepatic ischaemia/
/reperfusion (I/R) injury.
Materials and methods: The liver I/R was established in male C57BL/6 mice. The
effect of LDN is initially investigated on hepatic I/R injury via estimating histopathology
of liver. The level of metabolic enzymes alanine aminotransferase (ALT),
aspartate aminotransferase (AST) and alkaline phosphatase (ALP) was studied
along with apoptosis of mouse hepatocytes via TUNEL and flow cytometry analysis.
The effect of LDN was investigated on oxidative stress biomarkers (glutathione
[GSH] content, malondialdehyde [MDA] and superoxide dismutase [SOD] activities)
and pro-inflammatory cytokines (tumour necrosis factor alpha [TNF-α], interleukin
[IL]-1β and IL-6). Western blot study was also conducted to elucidate the effect
of LDN on toll-like receptor 4/nuclear factor kappa B (TLR4/NF-kB).
Results: Liriodendrin alleviates liver I/R injury, as manifested by decreased plasma
ALT, AST and ALP with improvement in liver necrotic area. LDN also reduces apoptosis
of mouse hepatocytes with reduction of oxidative stress and generation
of pro-inflammatory cytokines. It significantly reduces the expression of TLR4
and NF-kB.
Conclusions: The study demonstrated that LDN reduces liver injury and prevented
apoptosis of hepatocytes following I/R injury. In addition, LDN also reduces
oxidative stress, inflammation, and TLR4/NF-kB in I/R injured mice.

Abstract

Background: The aim of the present study was to investigate the protective effect
and mechanism of liriodendrin (LDN) is a lignan diglucoside in hepatic ischaemia/
/reperfusion (I/R) injury.
Materials and methods: The liver I/R was established in male C57BL/6 mice. The
effect of LDN is initially investigated on hepatic I/R injury via estimating histopathology
of liver. The level of metabolic enzymes alanine aminotransferase (ALT),
aspartate aminotransferase (AST) and alkaline phosphatase (ALP) was studied
along with apoptosis of mouse hepatocytes via TUNEL and flow cytometry analysis.
The effect of LDN was investigated on oxidative stress biomarkers (glutathione
[GSH] content, malondialdehyde [MDA] and superoxide dismutase [SOD] activities)
and pro-inflammatory cytokines (tumour necrosis factor alpha [TNF-α], interleukin
[IL]-1β and IL-6). Western blot study was also conducted to elucidate the effect
of LDN on toll-like receptor 4/nuclear factor kappa B (TLR4/NF-kB).
Results: Liriodendrin alleviates liver I/R injury, as manifested by decreased plasma
ALT, AST and ALP with improvement in liver necrotic area. LDN also reduces apoptosis
of mouse hepatocytes with reduction of oxidative stress and generation
of pro-inflammatory cytokines. It significantly reduces the expression of TLR4
and NF-kB.
Conclusions: The study demonstrated that LDN reduces liver injury and prevented
apoptosis of hepatocytes following I/R injury. In addition, LDN also reduces
oxidative stress, inflammation, and TLR4/NF-kB in I/R injured mice.

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Keywords

liriodendrin, liver ischaemia/reperfusion injury, apoptosis, inflammation

About this article
Title

Protective effect of liriodendrin against liver ischaemia/reperfusion injury in mice via modulating oxidative stress, inflammation and nuclear factor kappa B/toll-like receptor 4 pathway

Journal

Folia Morphologica

Issue

Vol 82, No 3 (2023)

Article type

Original article

Pages

668-676

Published online

2022-04-28

Page views

1190

Article views/downloads

669

DOI

10.5603/FM.a2022.0049

Pubmed

35607873

Bibliographic record

Folia Morphol 2023;82(3):668-676.

Keywords

liriodendrin
liver ischaemia/reperfusion injury
apoptosis
inflammation

Authors

Z. Y. Yu
G. Cheng

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