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Original article
Submitted: 2021-11-24
Accepted: 2021-12-20
Published online: 2021-12-31
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Anoctamin1 and c-Kit immunohistochemical study of interstitial cells of Cajal in the muscularis externa of human gastrointestinal tract

M. Atteya1, R. A. Mohammed1, A. Al-Ahmadi2, T. A. Al-Matrafi1, M. A. Alfayez1
DOI: 10.5603/FM.a2021.0138
·
Pubmed: 35037698
Affiliations
  1. Department of Anatomy, College of Medicine, King Saud University, Riyadh, Saudi Arabia
  2. Department of Chemistry, College of Science, King Abdulaziz University, Jeddah, Saudi Arabia

open access

Ahead of Print
ORIGINAL ARTICLES
Submitted: 2021-11-24
Accepted: 2021-12-20
Published online: 2021-12-31

Abstract

Background: Interstitial cells of Cajal )ICC) are widely distributed in human gastrointestinal (GI) tract  specially in the layer of muscularis externa between neurons and smooth muscles. They play a very important role of coordination of GI tract motility. The aims of this research were to study the morphology and distribution of ICC in the muscularis externa of the GI tract, using immunohistochemistry staining methods, to determine the distribution of immune reactivity of Anoctamin1 (Ano1) compared with c-Kit, and to determine if Ano1 is a reliable marker for ICC in human GI tract.

Materials and methods: Specimens from the wall of stomach, small intestine, and colon were taken from human cadavers and processed for histological and immunohistochemical study using c-Kit and Ano1 primary antibodies.

Results: ICC appeared as bipolar cells, not forming network, in both the circular and longitudinal muscle layers, while in the myenteric area they appeared as multipolar interconnected cells. They were unevenly distributed in and between the muscle layers of the muscularis externa of human GI tract. They were more numerous in the stomach followed by the colon then the small intestine, and more numerous in the myenteric area followed by the circular muscle layer then the longitudinal muscle layer, in the three organs. Our results also showed that Ano1 is a more reliable marker for human ICC than c-kit.

Conclusions: ICC differed in morphology and were unevenly distributed between muscle layers of muscularis externa and between different parts of human GI tract.

Abstract

Background: Interstitial cells of Cajal )ICC) are widely distributed in human gastrointestinal (GI) tract  specially in the layer of muscularis externa between neurons and smooth muscles. They play a very important role of coordination of GI tract motility. The aims of this research were to study the morphology and distribution of ICC in the muscularis externa of the GI tract, using immunohistochemistry staining methods, to determine the distribution of immune reactivity of Anoctamin1 (Ano1) compared with c-Kit, and to determine if Ano1 is a reliable marker for ICC in human GI tract.

Materials and methods: Specimens from the wall of stomach, small intestine, and colon were taken from human cadavers and processed for histological and immunohistochemical study using c-Kit and Ano1 primary antibodies.

Results: ICC appeared as bipolar cells, not forming network, in both the circular and longitudinal muscle layers, while in the myenteric area they appeared as multipolar interconnected cells. They were unevenly distributed in and between the muscle layers of the muscularis externa of human GI tract. They were more numerous in the stomach followed by the colon then the small intestine, and more numerous in the myenteric area followed by the circular muscle layer then the longitudinal muscle layer, in the three organs. Our results also showed that Ano1 is a more reliable marker for human ICC than c-kit.

Conclusions: ICC differed in morphology and were unevenly distributed between muscle layers of muscularis externa and between different parts of human GI tract.

Get Citation

Keywords

ICC, c-Kit; anoctamin1, stomach, small intestine, colon

About this article
Title

Anoctamin1 and c-Kit immunohistochemical study of interstitial cells of Cajal in the muscularis externa of human gastrointestinal tract

Journal

Folia Morphologica

Issue

Ahead of Print

Article type

Original article

Published online

2021-12-31

Page views

274

Article views/downloads

149

DOI

10.5603/FM.a2021.0138

Pubmed

35037698

Keywords

ICC
c-Kit
anoctamin1
stomach
small intestine
colon

Authors

M. Atteya
R. A. Mohammed
A. Al-Ahmadi
T. A. Al-Matrafi
M. A. Alfayez

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