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Porcine coronary arteries: immunohistochemical profile of TNF-alpha, IL-1beta, TGF-beta1 and ICAM-1
Affiliations- Department of Sensory Organs, Sapienza University of Rome, Italy
- Department of Anatomical, Histological, Forensic Medicine and Orthopaedics Sciences, Sapienza University of Rome, Italy
- Department of Radiological, Oncological and Pathological Sciences, Sapienza University of Rome, Italy
- UOC of Pathology, Sandro Pertini Hospital, Rome, Italy, Italy
- Department of Human Neurosciences, Sapienza University of Rome, Italy
- Department of Motor Sciences and Wellness, University of Naples “Parthenope”, Naples, Italy
- Department of Emergency Medicine, Foundation of Policlinico Agostino Gemelli-IRCCS, Catholic University of Sacred Heart, Rome, Italy
- Department of Anaesthesia and Critical Care, AON SS Antonio e Biagio e Cesare Arrigo, Alessandria, Italy
- Foundation Ospedale Alba-Bra and Department of Anaesthesia, Critical Care and Emergency Medicine, Pietro and Michele Ferrero Hospital, Verduno, Italy
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Abstract
Background: In our study we used immunohistochemical technique to demonstrate the presence of the cytokines tumour necrosis factor alpha (TNF-α), interleukin 1beta (IL-1β), transforming growth factor beta1 (TGF-β1) and intercellular adhesion molecule-1 (ICAM-1) in porcine coronaries even in physiological conditions.
Materials and methods: Inflammatory cytokines are polypeptide mediators which act as a communication signal between immune system cells and other types of cellsin different organs and tissues, both in human and pig coronary circulation.
Results: Our results show that pro-inflammatory cytokines TNF-α, IL-1β, TGF-β1 and ICAM-1 are also present in the medium tunica of the coronary arteries under physiological conditions. These results may be compared with those found in coronary atherosclerosis, where the increase in TNF-α has a dramatic effect on the function of the left ventricle, and the high value of IL-1 correlates directly with the extent of myocardial necrosis. In our study we observe the damage and activation of endothelial cells; this induces endothelial dysfunction by accumulation and oxidation of low density lipoproteins (LDL). The formation of oxidized LDL could play a central role in the amplification of the inflammatory response causing an increased expression of pro-inflammatory cytokines which promotes leukocyte recruitment in the intimal layer. These leukocytes, after the adhesion to the endothelium, penetrate the intimate tunic.
Conclusions: Therefore inflammatory processes promote the onset and evolution of atheroma and the development of thrombotic complications.
Abstract
Background: In our study we used immunohistochemical technique to demonstrate the presence of the cytokines tumour necrosis factor alpha (TNF-α), interleukin 1beta (IL-1β), transforming growth factor beta1 (TGF-β1) and intercellular adhesion molecule-1 (ICAM-1) in porcine coronaries even in physiological conditions.
Materials and methods: Inflammatory cytokines are polypeptide mediators which act as a communication signal between immune system cells and other types of cellsin different organs and tissues, both in human and pig coronary circulation.
Results: Our results show that pro-inflammatory cytokines TNF-α, IL-1β, TGF-β1 and ICAM-1 are also present in the medium tunica of the coronary arteries under physiological conditions. These results may be compared with those found in coronary atherosclerosis, where the increase in TNF-α has a dramatic effect on the function of the left ventricle, and the high value of IL-1 correlates directly with the extent of myocardial necrosis. In our study we observe the damage and activation of endothelial cells; this induces endothelial dysfunction by accumulation and oxidation of low density lipoproteins (LDL). The formation of oxidized LDL could play a central role in the amplification of the inflammatory response causing an increased expression of pro-inflammatory cytokines which promotes leukocyte recruitment in the intimal layer. These leukocytes, after the adhesion to the endothelium, penetrate the intimate tunic.
Conclusions: Therefore inflammatory processes promote the onset and evolution of atheroma and the development of thrombotic complications.
Keywords
porcine coronary arteries, cytokines, IL-1beta, TNF-alpha, TGF-beta1, ICAM-1
About this articleTitle
Porcine coronary arteries: immunohistochemical profile of TNF-alpha, IL-1beta, TGF-beta1 and ICAM-1
Journal
Issue
Article type
Original article
Pages
119-126
Published online
2021-12-16
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3520
Article views/downloads
1111
DOI
Pubmed
Bibliographic record
Folia Morphol 2023;82(1):119-126.
Keywords
porcine coronary arteries
cytokines
IL-1beta
TNF-alpha
TGF-beta1
ICAM-1
Authors
S. Taurone
M. T. Santarelli
E. De Santis
C. Di Gioia
E. Pompili
F. Pellegrino
P. Familiari
V. Papa
C. Zanza
L. Coppola
G. Familiari
M. Artico
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